The wheat AGC kinase TaAGC1 is a positive contributor to host resistance to the necrotrophic pathogen Rhizoctonia cerealis
文献类型: 外文期刊
第一作者: Zhu, Xiuliang
作者: Zhu, Xiuliang;Yang, Kun;Wei, Xuening;Rong, Wei;Du, Lipu;Ye, Xingguo;Qi, Lin;Zhang, Zengyan;Zhang, Qiaofeng
作者机构:
关键词: AGC kinase;differential expression;reactive oxygen species;resistance;Rhizoctonia cerealis;Triticum aestivum;wheat
期刊名称:JOURNAL OF EXPERIMENTAL BOTANY ( 影响因子:6.992; 五年影响因子:7.86 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: Considerable progress has been made in understanding the roles of AGC kinases in mammalian systems. However, very little is known about the roles of AGC kinases in wheat (Triticum aestivum). The necrotrophic fungus Rhizoctonia cerealis is the major pathogen of the destructive disease sharp eyespot of wheat. In this study, the wheat AGC kinase gene TaAGC1, responding to R. cerealis infection, was isolated, and its properties and role in wheat defence were characterized. R. cerealis-resistant wheat lines expressed TaAGC1 at higher levels than susceptible wheat lines. Sequence and phylogenetic analyses showed that the TaAGC1 protein is a serine/threonine kinase belonging to the NDR (nuclear Dbf2-related) subgroup of AGC kinases. Kinase activity assays proved that TaAGC1 is a functional kinase and the Asp-239 residue located in the conserved serine/threonine kinase domain of TaAGC1 is required for the kinase activity. Subcellular localization assays indicated that TaAGC1 localized in the cytoplasm and nucleus. Virus-induced TaAGC1 silencing revealed that the down-regulation of TaAGC1 transcripts significantly impaired wheat resistance to R. cerealis. The molecular characterization and responses of TaAGC1 overexpressing transgenic wheat plants indicated that TaAGC1 overexpression significantly enhanced resistance to sharp eyespot and reduced the accumulation of reactive oxygen species (ROS) in wheat plants challenged with R. cerealis. Furthermore, ROS-scavenging and certain defence-associated genes were up-regulated in resistant plants overexpressing TaAGC1 but down-regulated in susceptible knock-down plants. These results suggested that the kinase TaAGC1 positively contributes to wheat immunity to R. cerealis through regulating expression of ROS-related and defence-associated genes.
分类号: Q94
- 相关文献
作者其他论文 更多>>
-
Design and Experimentation of Mountain-type Pre-cutting Sugarcane Planter and Its Key Components Based on DEM
作者:Zhang, Shunsheng;Gao, Xinxin;Liu, Gaoyuan;Guo, Jiawen;Li, Xiaoyu;Yang, Kun;Li, Mingchun;Liu, Kai;Kong, Yixun
关键词:Sugarcane; Planter; Furrowing and soil covering device; Parameter calibration; EDEM
-
Metagenomic approaches reveal the variations of microbiota structures and functions with the growth of Melanaphis sacchari
作者:Wang, Yunchao;Li, Jingjing;Wu, Tinghui;Ruan, Yinwei;Ning, Zhiyuan;Yang, Kun;Lu, Yanhui;Guo, Jiawen;Xiao, Zhongjiu
关键词:Melanaphis sacchari; Bacterial microbiota; Diversity index; Bacterial function prediction
-
Rutin Attenuates Oxidative Stress Responses and Hepatocyte Metabolomics in β-Hydroxybutyric Acid-Induced Hepatocyte Injury in Calves
作者:Yang, Kun;Zhao, Haixia;Li, Dabiao;Yang, Kun;Zhao, Haixia;Gao, Min;Hu, Honglian
关键词:rutin; metabolomics; BHBA; oxidative stress
-
Differential impacts of lime and nitrogen amendment on nitrification, ammonia oxidizers, nitrite oxidizers, and bacterial communities in two acidic soils
作者:Wang, Qing;Xin, Yu;Ruan, Yunze;Wang, Qing;Xin, Yu;Zhang, Zhijun;Yang, Kun;Wang, Suiliang;Yu, Zhiyong;Qu, Junfeng
关键词:Nitrification; Soil pH; Lime; Ammonium sulfate; Nitrifiers; Bacterial community
-
Selection of dysfunctional alleles of bHLH1 and MYB1 has produced white grain in the tribe Triticeae
作者:Pei, Jiawei;Heng, Yanfang;Chen, Zhuo;Xiao, Qingmeng;Cao, Ying;Liu, Zhijin;Ma, Ligeng;Wang, Zheng;Li, Jian;He, Hang;Deng, Xing Wang;Wang, Ke;Ye, Xingguo;Hu, Zhaorong
关键词:grain color; evolution; selection; bHLH; MYB; wheat; Triticeae
-
Origin and de novo domestication of sweet orange
作者:Liu, Shengjun;Xu, Yuantao;Yang, Kun;Huang, Yue;Lu, Zhihao;Chen, Shulin;Gao, Xiang;Xiao, Gongao;Wang, Lun;Zheng, Weikang;Liu, Zishuang;Liao, Guanglian;He, Fa;Liu, Junjie;Wan, Pengfei;Ye, Junli;Jiao, Wenbiao;Chai, Lijun;Pan, Zhiyong;Zhang, Fei;Lin, Zongcheng;Guo, Wenwu;Larkin, Robert M.;Xie, Zongzhou;Wang, Xia;Deng, Xiuxin;Xu, Qiang;Liu, Shengjun;Xu, Yuantao;Yang, Kun;Lu, Zhihao;Zheng, Weikang;Wang, Xia;Xu, Qiang;Chen, Peng;Zeng, Xiuli;Ding, Fang;Zan, Yanjun
关键词:
-
Integrated transcriptomic and metabolomic analysis of goose epididymis reveals molecular markers associated with sperm mobility
作者:Ren, Xiaofang;Jiang, Ke;Yin, Jiaxin;Liu, Shengjun;Liu, Shengjun;Ma, Zhigang;Chen, Zhifeng;Yang, Kun
关键词:Zi goose; Epididymis; Sperm mobility; Transcriptomics; Metabolomics