Heterologous coexpression of Vitreoscilla hemoglobin and Bacillus megaterium glucanase in Streptomyces lydicus A02 enhanced its production of antifungal metabolites
文献类型: 外文期刊
第一作者: Wu, Huiling
作者: Wu, Huiling;Li, Jinjin;Dong, Dan;Liu, Ting;Zhang, Taotao;Zhang, Dianpeng;Liu, Weicheng
作者机构:
关键词: Vitreoscilla hemoglobin;Glucanase;Coexpression;Natamycin;Antifungal activity;Chitinase
期刊名称:ENZYME AND MICROBIAL TECHNOLOGY ( 影响因子:3.493; 五年影响因子:3.699 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: Streptomyces lydicus A02 is a novel producer of commercially important polyene macrocyclic antibiotic natamycin and a potential biocontrol agent to several plant fungal diseases, including wilt caused by Fusarium oxysporum f. spp. To improve the natamycin production and the antifungal activity of S. lydicus A02, we coexpressed gene vgb encoding Vitreoscilla hemoglobin (VHb) and bgiC encoding Bacillus megaterium L103 glucanase, both under the control of the strong constitutive ermE* promoter, in S. lydicus A02. Our results showed that coexpressingVHb and glucanase improved cell growth, and the engineered strain produced 26.90% more biomass than the wild-type strain after 72 h fermentation in YSG medium. In addition, coexpressing genes encoding VHb. and glucanase led to increased natamycin production, higher endogenous chitinase activity and exogenous glucanase activity, as well as enhanced antifungal activity in the engineered S. lydicus AVG02 and AGV02, regardless of the position of the two genes on the plasmids. Compared with model strains, few reports have successfully coexpressed VHb and other foreign proteins in industrial strains. Our results illustrated an effective approach for improving antifungal activity in an industrial strain by the rational engineering of combined favorable factors. (C) 2015 Elsevier Inc. All rights reserved.
分类号: Q5
- 相关文献
作者其他论文 更多>>
-
Construction of gastric-stable emulsions based on corn components: Impact of interfacial rheological properties on the dynamic bioaccessibility of lutein
作者:Wang, Yanqi;Chen, Yuying;Liu, Ting;Zheng, Jinkai;Wang, Yanqi;Zhu, Siyue;Dong, Qiaoru;Huang, Qingrong;Zheng, Jinkai
关键词:Emulsions; In vitro digestion; Interfacial rheological properties; Stabilization mechanism; Bioaccessibility
-
The nickel hyperaccumulator Odontarrhena chalcidica (Brassicaceae) preferentially takes up zinc over nickel
作者:Wang, Ya-Zhou;Geng, Ke-Rui;Tang, Ye-Tao;Mo, Bing-Lan;Liu, Ting;Qiu, Rong-Liang;Wen, Dian;Li, Fu-Rong;Wang, Xu;Li, Lei;Du, Rui-Ying;Deng, Teng-Hao-Bo;van der Ent, Antony;Morel, Jean-Louis
关键词:Nickel; Zinc; Hyperaccumulator; Uptake; Kinetics; Transporters
-
The instability of pectin-based emulsions in the upper digestive tract investigated based on the molecular structure and interfacial properties of pectin
作者:Du, Yizheng;Chen, Yuying;Liu, Ting;Ma, Mengyu;Feng, Liping;Zheng, Jinkai;Zheng, Jinkai
关键词:Pectin; Emulsion; Digestive treatment; Chemical structure; Interfacial properties
-
Characterization and fungicides sensitivity of Colletotrichum species causing Hydrangea macrophylla anthracnose in Beijing, China
作者:Zhao, Juan;Cheng, Yanli;Liu, Yayong;Zhang, Taotao;Qin, Wentao;Zhao, Juan;Liu, Yayong;Zhang, Taotao;Qin, Wentao;Cheng, Yanli;Shi, Xiaojing
关键词:
Hydrangea macrophylla ; anthracnose;Colletotrichum species; multi-loci phylogeny; fungicide sensitivity -
Long-term cadmium stress influenced the immune response of Pardosa pseudoannulata by modulating its gut microbiota and hemolymph metabolome
作者:Li, Jinjin;Zhou, Rong;Peng, Jie;She, Qingling;Yang, Xinyi;Wang, Juan;Wang, Zhi;Peng, Yuan-de;Song, Qisheng
关键词:Pardosa pseudoannulata; Cadmium; Gut microbiota; Hemolymph; Immune system
-
Effects of Bacillus subtilis addition to milk replacer on growth performance, nutrient digestibility, intestinal microbiota, and short-chain fatty acid concentration of Hu lambs
作者:Yang, Xinhui;Liu, Ting;Zhou, Juwang;Zheng, Chen;An, Lijing;Pan, Faming;Pan, Faming;Zhang, Hui;Wang, Xinji;Xu, Guoyan
关键词:Milk replacer; Bacillus subtilis; Intestinal microbiota; Diarrhea; Lamb growth performance
-
Size-dependent uptake of chromium colloids in rice (Oryza sativa L.)
作者:Li, Jingjing;Guo, Weichao;Xu, Kai;Mo, Yijun;Jin, Chao;Tang, Yetao;Qiu, Rongliang;Xie, Weipeng;Liu, Ting;Qiu, Rongliang;Ao, Ming;Sun, Shengsheng;Deng, Tenghaobo
关键词:Chromium uptake; Rice; Colloidal particles; Endocytosis