Effects of nitrite exposure on haematological parameters, oxidative stress and apoptosis in juvenile turbot (Scophthalmus maximus)
文献类型: 外文期刊
第一作者: Jia, Rui
作者: Jia, Rui;Huo, Huan-Huan;Jia, Rui;Lei, Ji-Lin;Liu, Bao-Liang;Huang, Bin;Yin, Shu-Ting;Han, Cen
作者机构:
关键词: Nitrite;Oxidative stress;Apoptosis;Gills;Scophthalmus maximus
期刊名称:AQUATIC TOXICOLOGY ( 影响因子:4.964; 五年影响因子:5.071 )
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收录情况: SCI
摘要: Nitrite (NO2-) is commonly present as contaminant in aquatic environment and toxic to aquatic organisms. In the present study, we investigated the effects of nitrite exposure on haematological parameters, oxidative stress and apoptosis in juvenile turbot (Scophthalmus maximus). Fish were exposed to various concentrations of nitrite (0, 0.02, 0.08, 0.4 and 0.8 mM) for 96 h. Fish blood and gills were collected to assay haematological parameters, oxidative stress and expression of genes after 0, 24, 48 and 96 h of exposure. In blood, the data showed that the levels of methemoglobin (MetHb), triglyceride (TG), potassium (K+), cortisol, heat shock protein 70 (HSP70) and glucose significantly increased in treatments with higher concentrations of nitrite (0.4 and/or 0.8 mM) after 48 and 96 h, while the levels of haemoglobin (Hb) and sodium (Na+) significantly decreased in these treatments. In gills, nitrite (0.4 and/or 0.8 mM) apparently reduced the levels of superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) and glutathione (GSH), increased the formation of malondialdehyde (MDA), up-regulated the mRNA levels of c-jun amino-terminal kinase (JUK1), p53, caspase-3, caspase-7 and caspase-9 after 48 and 96 h of exposure. The results suggested caspase-dependent and JUK signaling pathways played important roles in nitrite-induced apoptosis in fish. Further, this study provides new insights into how nitrite affects the physiological responses and apoptosis in a marine fish. (C) 2015 Elsevier B.V. All rights reserved.
分类号: Q17
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