High Oxygen Concentration Increases the Abundance and Activity of Bacterial Rather than Archaeal Nitrifiers in Rice Field Soil

文献类型： 外文期刊

第一作者： Ke, Xiubin

作者： Ke, Xiubin;Lu, Wei;Ke, Xiubin;Conrad, Ralf

作者机构：

关键词： Nitrification rate;Ammonia-oxidizing bacteria;Ammonium-oxidizing archaea;Nitrite-oxidizing bacteria;Ammonia monooxygenase;Nitrospira;Nitrobacter;Transcription;T-RFLP;qPCR

期刊名称：MICROBIAL ECOLOGY （ 影响因子：4.552； 五年影响因子：4.77 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Oxygen is considered as a limiting factor for nitrification in rice paddy soil. However, little is known about how the nitrifying microbial community responds to different oxygen concentrations at community and transcript level. In this study, soil and roots were harvested from 50-day-old rice microcosms and were incubated for up to 45 days under two oxygen concentrations: 2 % O-2 and 20 % O-2 (ambient air). Nitrification rates were measured from the accumulation of nitrite plus nitrate. The population dynamics of bacterial (AOB) and archaeal (AOA) ammonia oxidizers was determined from the abundance (using quantitative PCR (qPCR)) and composition (using terminal restriction fragment length polymorphism and cloning/sequencing) of their amoA genes, that of nitrite oxidizers (NOB) by quantifying the nxrA gene of Nitrobacter spp. and the 16S rRNA gene of Nitrospira spp. The activity of the nitrifiers was determined by quantifying the copy numbers of amoA and nxrA transcripts (using RT-qPCR). Different oxygen concentrations did not affect the community compositions of AOB, AOA, and NOB, which however were different between surface soil, bottom soil, and rice roots. However, nitrification rates were higher under ambient air than 2 % O-2, and abundance and transcript activities of AOB, but not of AOA, were also higher. Abundance and transcript copy numbers of Nitrobacter were also higher at ambient air. These results indicate that AOB and NOB, but not AOA, were sensitive to oxygen availability.

分类号： Q938

  • 相关文献

[1]Variable responses of ammonia oxidizers across soil particle-size fractions affect nitrification in a long-term fertilizer experiment. Zhang, Qian,Liang, Guoqing,Zhou, Wei,Myrold, David D..

[2]Different behaviour of methanogenic archaea and Thaumarchaeota in rice field microcosms. Ke, Xiubin,Lu, Yahai,Ke, Xiubin,Conrad, Ralf,Ke, Xiubin.

[3]Long-term organic and inorganic fertilization alters temperature sensitivity of potential N2O emissions and associated microbes. Cui, Peiyuan,Fan, Fenliang,Yin, Chang,Song, Alin,Liang, Yongchao,Huang, Pingrong,Tang, Yongjun,Zhu, Ping,Peng, Chang,Li, Tingqiang,Liang, Yongchao,Wakelin, Steven A..

[4]SOIL RESPIRATION AND NITRIFICATION-DENITRIFICATION IN MAIZE/SOYBEAN INTERCROPPING SYSTEM. Gao, Yang,Li, Xinqiang,Shen, Xiaojun,Sun, Jingsheng,Duan, Aiwang.

[5]Vertical Segregation and Phylogenetic Characterization of Ammonia-Oxidizing Bacteria and Archaea in the Sediment of a Freshwater Aquaculture Pond. Lu, Shimin,Liu, Xingguo,Zeng, Xianlei,Shi, Xu,Gu, Zhaojun,Lu, Shimin,Liu, Qigen,Zeng, Xianlei,Ma, Zhuojun,Wu, Zongfan. 2016

[6]Abundance and Community Composition of Ammonia-Oxidizers in Paddy Soil at Different Nitrogen Fertilizer Rates. Song Ya-na,Lin Zhi-min. 2014

[7]Different roles of rhizosphere effect and long-term fertilization in the activity and community structure of ammonia oxidizers in a calcareous fluvo-aquic soil. Ai, Chao,Liang, Guoqing,Sun, Jingwen,Wang, Xiubin,He, Ping,Zhou, Wei. 2013

[8]Ammonia adsorption and nitritation in sediments derived from the Three Gorges Reservoir, China. Wang, Hongyuan,Shen, Zhenyao,Guo, Xuejun,Niu, Junfeng,Wang, Hongyuan,Kang, Bin. 2010

[9]Effect of ammonium nitrogen concentration on the ammonia-oxidizing bacteria community in a membrane bioreactor for the treatment of anaerobically digested swine wastewater. Sui, Qianwen,Liu, Chong,Dong, Hongmin,Zhu, Zhiping,Sui, Qianwen,Liu, Chong,Dong, Hongmin,Zhu, Zhiping.

[10]Effects of myclobutanil on soil microbial biomass, respiration, and soil nitrogen transformations. Xu, Jun,Wu, Xiaohu,Dong, Fengshou,Liu, Xingang,Zheng, Yongquan.

[11]Community composition of ammonia-oxidizing bacteria in the rhizosphere of intercropped wheat (Triticum aestivum L.), maize (Zea mays L.), and faba bean (Vicia faba L.). Song, Y. N.,Marschner, P.,Li, L.,Bao, X. G.,Sun, J. H.,Zhang, F. S.. 2007

[12]Direct and indirect influence of arbuscular mycorrhizal fungi on abundance and community structure of ammonia oxidizing bacteria and archaea in soil microcosms. Chen, Yong-Liang,Chen, Bao-Dong,Hu, Ya-Jun,Li, Tao,Zhang, Xin,Hao, Zhi-Peng,Wang, You-Shan.

[13]Long-term fertilization effects on active ammonia oxidizers in an acidic upland soil in China. Wang, Xinli,Zhong, Wenhui,Wang, Xinli,Han, Cheng,Zhang, Jinbo,Deng, Huan,Deng, Yongcui,Zhong, Wenhui,Han, Cheng,Zhang, Jinbo,Deng, Huan,Deng, Yongcui,Zhong, Wenhui,Huang, Qianru.

[14]Removing ammonium from aquaculture ponds using suspended biocarrier-immobilized ammonia-oxidizing microorganisms. Lu, Shimin,Liao, Mingjun,Xie, Congxin,He, Xugang,Li, Dapeng,Liu, Quansheng,Li, Ruijiao,Lu, Shimin,Liao, Mingjun,Li, Ruijiao.

[15]The effects of different disease-resistant cultivars of banana on rhizosphere microbial communities and enzyme activities. Sun, Jianbo,Peng, Ming,Wang, Yuguang,Li, Wenbin,Xia, Qiyu.

[16]Effect of cypermethrin insecticide on the microbial community in cucumber phyllosphere. Zhang Baoguo,Zhang Hongxun,Tang Ling,Yang Jianzhou,Zhuang Guoqiang,Bai Zhihui,Jin Bo,Bai Zhihui,Zhang Baoguo,Zhang Hongxun,Li Baoju.

[17]Improved fermentation performance in an expanded ectopic fermentation system inoculated with thermophilic bacteria. Guo, Hui,Peng, Xiawei,Zhu, Changxiong,Geng, Bing,Liu, Xue,Ye, Jing,Tian, Yunlong.

[18]Linking plant identity and interspecific competition to soil nitrogen cycling through ammonia oxidizer communities. Fan, Fenliang,Zhang, Fusuo,Lu, Yahai,Fan, Fenliang. 2011

[19]Different denitrification potential of aquic brown soil in Northeast China under inorganic and organic fertilization accompanied by distinct changes of nirS- and nirK-denitrifying bacterial community. Yin, Chang,Fan, Fenliang,Song, Alin,Li, Zhaojun,Liang, Yongchao,Yu, Wantai,Liang, Yongchao.

[20]Diversity and space-time dynamics of endophytic archaea from sugar beet in the north slope of Tianshan Mountain revealed by 454 pyrosequencing and T-RFLP. Shi, YingWu,TaPa, MuSi,Li, Chun,Yang, HongMei,Zhang, Tao,Gao, Yan,Sun, Jian,Zeng, Jun,Lin, Qing,Cao, ZhenHua,OuTi, KuEr,Li, YuGuo,Lou, Kai,Shi, YingWu,TaPa, MuSi,Li, Chun,Yang, HongMei,Zhang, Tao,Gao, Yan,Sun, Jian,Zeng, Jun,Lin, Qing,Cao, ZhenHua,OuTi, KuEr,Li, YuGuo,Lou, Kai.

作者其他论文 更多>>

微信小程序