Direct Fluorescent Detection of a Polymethoxyflavone in Cell Culture and Mouse Tissue

文献类型： 外文期刊

第一作者： Chen, Jingjing

作者： Chen, Jingjing;Song, Mingyue;Wu, Xian;Zheng, Jinkai;He, Lili;McClements, David Julian;Decker, Eric;Xiao, Hang;Xiao, Hang;Zheng, Jinkai

作者机构：

关键词： polymethoxyflavone;fluorescence detection;colon cancer cell;mouse tissue;imaging

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：5.279； 五年影响因子：5.269 ）

ISSN：

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收录情况： SCI

摘要： Convenient detection methods for bioactive food compounds and their metabolites in biological samples are needed to better understand their mechanism of actions. Herein, we developed a novel approach to directly monitor and visualize the distribution of 5,3',4'-tridemethylnobiletin (TDN), a unique polymethoxyflavone metabolite derived from citrus polymethoxyflavone, in biological samples such as cultured cells and mouse colonic tissues. Our results showed that a fluorescent conjugate could be formed between TDN and 2-aminoethyl diphenyl borate (DPBA) under simple reaction conditions, which was confirmed by both Raman spectroscopy and mass spectroscopy. We further demonstrated the application of DPBA-based conjugation reaction in the characterization of TDN in different biological samples including floating cells, adherent cells, and animal tissues. This is the first report demonstrating direct fluorescent detection of polymethoxyflavone in biological samples.

分类号： R15`S

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