Development of blocking ELISA for detection of antibodies against H9N2 avian influenza viruses

文献类型: 外文期刊

第一作者: Li, Zejun

作者: Li, Zejun

作者机构:

关键词: Blocking ELISA;Antibodies;H9N2 subtype;Avian influenza virus

期刊名称:JOURNAL OF VIROLOGICAL METHODS ( 影响因子:2.014; 五年影响因子:2.001 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: A blocking enzyme-linked immunosorbent assay (bELISA) was developed for detection of antibodies against H9N2 avian influenza viruses (AB's) based on a monoclonal antibody specific to the hemagglutinin (HA) protein of H9N2 AIV. The specificity of the bELISA was tested using antisera against H3, H4, H5, H7, and H10 AIVs and other avian viruses. The average percent inhibition (PI) value of 116 non-immune serum samples from ducks and specific pathogen-free (SPF) chickens was 2.80%. The selected cut-off PI values for negative and positive sera were 17.6% and 25.0%, respectively. A high correlation of >96% was identified between the bELISA and hemagglutinin inhibition (HI) assay, according to the detection results of sera from infected chickens (n = 30) and from chickens vaccinated with an inactivated H9N2 vaccine (n = 40). Sera collected from vaccinated chickens and ducks (n = 660) at different weeks post vaccination that were positive by the HI assay were confirmed with the bELISA. The results revealed a time-dependent increase in antibody levels. Therefore, the bELISA offers the potential advantage of a high throughput, rapid, sensitive, and specific method for detection of specific antibodies against H9N2. (C) 2015 Elsevier B.V. All rights reserved.

分类号: R37

  • 相关文献

[1]Sequence analysis of the haemagglutinin genes of H9N2 avian influenza viruses isolated in China during 1996 to 2011. Dai, Yin,Zhao, Ruihong,Hu, Xiaomiao,Zhang, Danjun,Wang, Junjun,Xu, Fazhi. 2012

[2]Sequence and phylogenetic analysis of hemagglutinin genes of H9N2 influenza viruses isolated from chicken in China from 2013 to 2015. Su Xiao-na,Xie Qing-mei,Chen Wei-guo,Bi Ying-zuo,Chen Feng,Su Xiao-na,Xie Qing-mei,Chen Feng,Su Xiao-na,Xie Qing-mei,Chen Feng,Liao Chang-tao,Yan Zhuan-qiang. 2016

[3]Single-amino-acid mutation in the HA alters the recognition of H9N2 influenza virus by a monoclonal antibody. Ping, Jihui,Li, Chengjun,Deng, Guohua,Jiang, Yongping,Tian, Guobin,Bu, Zhigao,Chen, Hualan,Ping, Jihui,Zhang, Shuxia. 2008

[4]H9N2 avian influenza virus-derived natural reassortant H5N2 virus in swan containing the hemagglutinin segment from Eurasian H5 avian influenza virus with an in-frame deletion of four basic residues in the polybasic hemagglutinin cleavage site. Wang, Youling,Yuan, Xiaoyuan,Qi, Lihong,Zhang, Yuxia,Xu, Huaiying,Yang, Jinxing,Ai, Wu,Song, Minxun,Li, Feng,Qi, Wenbao,Liao, Ming,Wang, Dan,Li, Feng,Li, Feng.

[5]Transmission of avian H9N2 influenza viruses in a murine model. Wu, Rui,Liu, Zewen,Liang, Wangwang,Yang, Keli,Xiong, Zhongliang,Xu, Diping,Sui, Zhiwei,Wu, Rui,Xu, Diping.

[6]Antigenic and genetic analysis of the H9N2 avian influenza viruses isolated in China. Li, CJ,Tian, GB,Li, YB,Yu, DD,Yu, KZ,Chen, HL. 2004

[7]Expression, purification, and characterization of recombinant NS-1, the porcine parvovirus non-structural protein. Qi, Ting,Cui, Shang-jin.

[8]Synthesis of empty capsid-like particles of Asia I foot-and-mouth disease virus in insect cells and their immunogenicity in guinea pigs. Cao, Yimei,Lu, Zengjun,Sun, Jiachuan,Bai, Xingwen,Sun, Pu,Bao, Huifang,Chen, Yingli,Guo, Jianhong,Li, Dong,Liu, Xiangtao,Liu, Zaixin.

[9]Development of a blocking ELISA for detection of antibodies against avian hepatitis E virus. Liu, Baoyuan,Zhao, Qin,Sun, Yani,Wang, Xinjie,Zhao, Jinan,Du, Taofeng,Wang, Chengbao,Xiao, Shuqi,Mu, Yang,Zhang, Gaiping,Zhou, En-Min,Liu, Baoyuan,Zhao, Qin,Sun, Yani,Wang, Xinjie,Zhao, Jinan,Du, Taofeng,Wang, Chengbao,Xiao, Shuqi,Mu, Yang,Zhou, En-Min,Zhang, Gaiping,Luo, Jianxuri,Hsu, Walter H..

[10]A comparison of a blocking ELISA and a haernagglutination inhibition assay for the detection of antibodies to Avibacterium (Haemophilus) paragallinarum in sera from artificially infected chickens. Sun, H.,Miao, D.,Zhang, P.,Gong, Y.,Blackall, P. J.. 2007

[11]Development of a blocking ELISA for detection of Mycoplasma hyopneumoniae infection based on a monoclonal antibody against protein P65. Liu, Maojun,Zhang, Yue,Wu, Yuzi,Wang, Haiyan,Li, Bin,Bai, Yun,Feng, Zhixin,Xiong, Qiyan,Bai, Fangfang,Shao, Guoqing,Liu, Maojun,Du, Gaimei,Browning, Glenn F.,Shao, Guoqing.

[12]Development of a blocking ELISA for Caprine parainfluenza virus type. Mao, Li,Li, Wenliang,Zhou, Tianci,Yang, Leilei,Hao, Fei,Li, Jizong,Zhang, Wenwen,Jiang, Jieyuan,Mao, Li,Luo, Xuenong. 2017

[13]Development of a blocking ELISA for detection of serum neutralizing antibodies against porcine circovirus type 2. Huang, Liping,Lu, Yuehua,Wei, Yanwu,Guo, Longjun,Liu, Changming. 2011

[14]Evolution analysis of the matrix (M) protein genes of 17 H9N2 chicken influenza viruses isolated in northern China during 1998-2008. Huang, Yanyan,Hu, Beixia,Zhang, Xiumei,Wen, Xintian,Cao, Sanjie,Xu, Dong,Khan, Mazhar I.. 2009

[15]Protective efficacy of commercial Newcastle disease vaccines against challenge of goose origin virulent Newcastle disease virus in geese. Dai, Yabin,Liu, Mei,Li, Wenliang.

[16]Immunogenicity and protective efficacy in monkeys of purified inactivated Vero-cell SARS vaccine. Qin, E,Shi, HY,Tang, L,Wang, C,Chang, GH,Ding, ZF,Zhao, K,Wang, J,Chen, Z,Yu, M,Si, BY,Liu, JY,Wu, DL,Cheng, XJ,Yang, BA,Peng, WM,Meng, QW,Liu, BH,Han, WG,Yin, XA,Duan, HY,Zhan, DW,Tian, L,Li, SL,Wu, JS,Tan, G,Li, Y,Li, YC,Liu, YG,Liu, H,Lv, FS,Zhang, Y,Kong, XG,Fan, BC,Jiang, T,Xu, SL,Wang, XM,Li, CW,Wu, XH,Deng, YQ,Zhao, M,Zhu, QY.

[17]Quadruple antigenic epitope peptide producing immune protection against classical swine fever virus. Liu, Siguo,Yu, Xinglong,Wang, Chunlai,Wu, Jianmin,Kong, Xiangang,Tu, Changchun.

[18]Immune response in guinea pigs vaccinated with DNA vaccine of foot-and-mouth disease virus O/China99. Guo, HC,Liu, ZX,Sun, SQ,Bao, HF,Chen, YL,Liu, XT,Xie, QG.

[19]Fusion of C3d with hemagglutinin enhances protective immunity against swine influenza virus. Li, Guo-Xin,Tian, Zhi-Jun,Yu, Hai,Jin, Yuan-Yuan,Hou, Shao-Hua,Zhou, Yan-Jun,Liu, Tian-Qiang,Hu, Shou-Ping,Tong, Guang-Zhi,Li, Guo-Xin,Tong, Guang-Zhi,Li, Guo-Xin,Yu, Hai,Zhou, Yan-Jun,Tong, Guang-Zhi.

[20]Influence of mannan oligosaccharide, Ligustrum lucidum and Schisandra chinensis on parameters of antioxidative and immunological status of broilers. Ma, Deying,Li, Qundao,Du, Juan,Liu, Yuqin,Liu, Shengwang,Shan, Anshan.

作者其他论文 更多>>