Simultaneous quantification of six major triterpenoid saponins in Schefflera kwangsiensis using high-performance liquid chromatography coupled to orbitrap mass spectrometry

文献类型: 外文期刊

第一作者: Zhang, Leilei

作者: Zhang, Leilei;Zhang, Leilei;Wang, Yan;Yu, De-Quan;Wang, Yan;Yu, De-Quan;Wang, Yan

作者机构:

关键词: Schefflera kwangsiensis;triterpenoid saponins;quantitative analysis;HPLC-ESI-MS

期刊名称:NATURAL PRODUCT RESEARCH ( 影响因子:2.861; 五年影响因子:2.572 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: A simple and accurate analytical method was developed for simultaneous and quantitative analysis of six triterpenoid saponins in Schefflera kwangsiensis via high-performance liquid chromatography (HPLC) with mass spectrometry (MS) in this study. Separation was performed on a Thermo hypersil GOLD C_(18) column (150 mm X2.1mm, 5 mum). A mobile phase consisting of methanol/acetonitrile/8 mM ammonium acetate in water was used with a flow rate of 0.3 mL/min. The analytes were detected by MS with the electrospray ionisation (ESI) source combined with negative monitoring and full scan mode, and were analysed by extracted ion chromatography. This established HPLC-ESI-MS analysis demonstrated good linearity, sensitivity, stability, precision, accuracy and recovery. Therefore, this analytical method has great potential to be a novel tool to qualify S. kwangsiensis.

分类号: R9

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