Analysis of UB and L40 resistance related to deltamethrin in Drosophila kc cells

文献类型： 外文期刊

第一作者： Chi, Qingping

作者： Chi, Qingping;Liu, Wei;Hu, Junli;Cheng, Luogen;Li, Fengliang;Cheng, Ying;Jing, Xuejian;Xu, Qin

作者机构：

关键词： UB;L40;Deltamethrin;Cell transfection;RNA interference;Western;CCK-8;Drosophila kc cells

期刊名称：GENE （ 影响因子：3.688； 五年影响因子：3.329 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Pests have been subjected to heavy selection pressure, and the development of resistance to pyrethroid has been recorded. It is extremely valuable to identify the resistance genes that are relevant for pest control. In our previous studies, we reported that UBL40 is the deltamethrin resistance-associated gene. UBL40 is cleaved by specific endopeptidases to release UB and L40. Whether UB or L40 participates in deltamethrin resistance requires further study. In this study, quantitative real-time PCR was applied to reveal that UB and L40 were both overexpressed in Drosophila kc cells after deltamethrin stimulation. To investigate the roles of UB and L40 further, RNA interferences (RNAi) and cell transfections were utilized. UB and L40 knockdown both significantly reduced the level of resistance of RNAi-treated cells after 48 h, and the overexpressions of UB and L40 in Drosophila kc cells conferred a degree of protection against deltamethrin. These results represent the first evidence that UB and L40 both play roles in the regulation of deltamethrin resistance and that this study could help us to elucidate the resistance mechanisms and identify new target genes associated with resistance. (C) 2015 Elsevier B.V. All rights reserved.

分类号： R394

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