Effects of microfluidization with ionic liquids on the solubilization and structure of beta-D-glucan

文献类型： 外文期刊

第一作者： Liu, Hongzhi

作者： Liu, Hongzhi;Li, Yanan;Gao, Jie;Shi, Aimin;Liu, Li;Hu, Hui;Yu, Hongwei;Wang, Qiang;Fan, Wei;Putri, Novita

作者机构：

关键词： Yeast beta-D-glucan;Solubilization;Structure

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：6.953； 五年影响因子：6.737 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： In order to enhance the solubility of yeast beta-D-glucan and enlarge the applicable area, we investigated dynamic high pressure microfluidization (DHPM) treatment with ionic liquids as solvent to prepare water-soluble yeast beta-D-glucan (WSG). The solubility of beta-D-glucan was greatly impacted by the pressure of DHPM, residence time, and ratio of beta-D-glucan and EmimAc. The yield of WSG reached 79.25 % (w/w) with the water solubility of 72.50 % (w/w). The DHPM treatment did not destroy the ionic liquid structure therefore the ionic liquids could be recycled. FTIR and C-13 NMR spectrum results showed that WSG was the polysaccharides with (1 -> 3)-beta-D-glycosidic bonds, after modification, the hydrogen bonding was destroyed. The chain conformation of WSG in 0.1 M NaNO3 aqueous solutions at 25 degrees C were studied by laser light scattering and viscometry. The Mark-Houwink equation was established to be [eta] = 2.09 x 10(-2) M-W(0.63) (cm(3) g(-1)). According to the conformational parameters calculated from wormlike cylinder model, WSG existed as semi-stiff chain in aqueous solution. (C) 2015 Published by Elsevier B.V.

分类号： Q71

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