Characterization of polysaccharide from longan pulp as the macrophage stimulator

文献类型： 外文期刊

第一作者： Yi, Yang

作者： Yi, Yang;Wang, Hongxun;Min, Ting;Yi, Yang;Wang, Hongxun;Zhang, Ruifen;Huang, Fei;Liu, Lei;Zhang, Mingwei

作者机构：

期刊名称：RSC ADVANCES （ 影响因子：3.361； 五年影响因子：3.39 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Longan is one of the most popular subtropical fruits in Southeast Asia, because of its flavor and benefits to health. As one of the important active ingredients of longan pulp, polysaccharide LPIIa was obtained by hot water extraction, ion-exchange chromatography and gel filtration chromatography. Its physicochemical characterization and immunostimulatory effects on macrophages were then investigated. Structural analyses indicated that LPIIa was a 44.7 kDa heteropolysaccharide mainly composed of -> 6)-Glc-(1 ->, -> 5)-Ara-(1 ->, -> 4)-Man-(1 -> and -> 6)-Gal-(1 ->. It enhanced macrophage phagocytosis and nitric oxide production in the dose range of 100-400 mu g mL(-1). Moreover, it significantly increased the inducible nitric oxide synthase activity, tumor necrosis factor-alpha and interleukin-6 secretion of macrophages at 200 mu g mL(-1). However, these effects were obviously weakened after toll-like receptor 4 (TLR4) or TLR2 was blocked. Likewise, the specific inhibitors of p38 mitogen-activated protein kinase (MAPK), protein kinase C, phosphatidylinositol 3-kinase, protein tyrosine kinase and nuclear factor kappa B (NF-kappa B) selectively depressed the immunostimulatory activities of LPIIa on macrophages. LPIIa stimulated macrophage activation partly via TLR4 and TLR2, followed by p38 MAPK- and NF-kappa B-dependent signaling pathways. The results suggested that LPIIa possessed potent immunomodulatory activity by stimulating macrophages and could be used as an immunotherapeutic adjuvant.

分类号： O6

  • 相关文献
作者其他论文 更多>>

微信小程序