A new preparative method for simultaneous purification of ochratoxin A and ochratoxin B from wheat culture inoculated with Aspergillus ochraceus

文献类型： 外文期刊

第一作者： Zhao, Z. Y.

作者： Zhao, Z. Y.;Yang, L. C.;Zhou, Z. L.;Deng, Y. F.;Hou, J. F.;Zhao, Z. Y.;Liu, N.;Wu, A. B.;Song, S. Q.;Wang, J. H.

作者机构：

关键词： mycotoxin;ochratoxin;purification;acid-assisted liquid-liquid extraction;gel permeation chromatography

期刊名称：WORLD MYCOTOXIN JOURNAL （ 影响因子：3.353； 五年影响因子：3.108 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Ochratoxins are a mycotoxin family frequently found in agricultural commodities worldwide and pose a potential health risk to humans and animals. To obtain large amounts of high-purity ochratoxins for food safety monitoring and toxicological research, a novel and effective method was established for simultaneous purification of ochratoxin A (OTA) and ochratoxin B (OTB) from a wheat culture inoculated with an ochratoxin-producing Aspergillus strain. The inoculated wheat culture was first extracted with methanol: water (80:20, v/v), followed by one or two cleanup procedures involving acid-assisted liquid-liquid extraction and gel permeation chromatography. Subsequently, target analytes were separated and collected using preparative high performance liquid chromatography. Finally, a combined approach of ultra-high performance liquid chromatography, ultraviolet spectrophotometry and mass spectrometry was applied for purity analysis and structural identification of the obtained toxins. As a result, 100 g of an inoculated wheat culture yielded 69 mg of OTA and 6 mg of OTB with purities greater than 98%. This proposed method might serve as a valuable reference to obtain expensive ochratoxin standards. To the best of our knowledge, this is the first report on simultaneous preparation of OTA and OTB from artificially inoculated wheat culture.

分类号： Q93

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