A thermostable Gloeophyllum trabeum xylanase with potential for the brewing industry

文献类型: 外文期刊

第一作者: Wang, Xiaoyu

作者: Wang, Xiaoyu;Liu, Weina;Hou, Lingyu;Zheng, Fei;Xie, Xiangming;Wang, Xiaoyu;Luo, Huiying;Ma, Rui;You, Shuai;Zheng, Fei;Yao, Bin;Yu, Wangning

作者机构:

关键词: Gloeophyllum trabeum;Glycoside hydrolase family 10 (GH10);Xylanase;Thermo-tolerant

期刊名称:FOOD CHEMISTRY ( 影响因子:7.514; 五年影响因子:7.516 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: A xylanase gene of glycoside hydrolase family 10, GtXyn10, was cloned from Gloeophyllum trabeum CBS 900.73 and expressed in Pichia pastoris GS115. Purified recombinant GtXyn10 exhibited significant activities to xylan (100.0%), lichenan (11.2%), glucan (15.2%) and p-nitrophenol-beta-cellobiose (18.6%), demonstrated the maximum xylanase and glucanase activities at pH 4.5-5.0 and 75 degrees C, retained stability over the pH range of 2.0-7.5 and at 70 degrees C, and was resistant to pepsin and trypsin, most metal ions and SDS. Multiple sequence alignment and modeled-structure analysis identified a unique Gly48 in GtXyn10, and site-directed mutagenesis of Gly48 to Lys improved the temperature optimum up to 80 degrees C. Under simulated mashing conditions, GtXyn10 (80 U) reduced the mash viscosity by 12.8% and improved the filtration rate by 31.3%. All these properties above make GtXyn10 attractive for potential applications in the feed and brewing industries. (C) 2015 Elsevier Ltd. All rights reserved.

分类号: TS2`TS201.2

  • 相关文献

[1]Improvement of the catalytic performance of a hyperthermostable GH10 xylanase from Talaromyces leycettanus JCM12802. Wang, Xiaoyu,Huang, Huoqing,Ma, Rui,Bai, Yingguo,Zheng, Fei,You, Shuai,Zhang, Bingyu,Xie, Huifang,Yao, Bin,Luo, Huiying,Wang, Xiaoyu,Xie, Xiangming,Zheng, Fei.

[2]Biochemical characterization of an acidophilic beta-mannanase from Gloeophyllum trabeum CBS900.73 with significant transglycosylation activity and feed digesting ability. Wang, Caihong,Wang, Yuan,Niu, Canfang,Ma, Rui,Wang, Yaru,Bai, Yingguo,Luo, Huiying,Yao, Bin,Zhang, Jiankang,Ma, Rui.

[3]Cloning and characterization of a new cold-adapted and thermo-tolerant iota-carrageenase from marine bacterium Flavobacterium sp YS-80-122. Li, Shangyong,Hao, Jianhua,Sun, Mi,Li, Shangyong,Hao, Jianhua,Sun, Mi.

[4]Symbiotic Streptomyces sp. TN119 GH 11 xylanase: a new pH-stable, protease- and SDS-resistant xylanase. Zhou, Junpei,Shi, Pengjun,Zhang, Rui,Huang, Huoqing,Meng, Kun,Yang, Peilong,Yao, Bin. 2011

[5]Isolation and Identification of a Newly Isolated Alternaria sp ND-16 and Characterization of Xylanase. Li, Yin,Liu, Zhiqiang,Liu, Zhiqiang,Li, Geng,Cui, Fengjie,Ping, Lifeng,Qiu, Chongyan,Yan, Lijiao,Yan, Lijiao. 2009

[6]An acid and highly thermostable xylanase from Phialophora sp G5. Zhang, Fan,Shi, Pengjun,Bai, Yingguo,Luo, Huiying,Yuan, Tiezheng,Huang, Huoqing,Yang, Peilong,Yao, Bin,Zhang, Fan,Miao, Lihong. 2011

[7]Cloning, Expression, and Characterization of a New Streptomyces sp S27 Xylanase for Which Xylobiose is the Main Hydrolysis Product. Li, Ning,Shi, Pengjun,Yang, Peilong,Wang, Yaru,Luo, Huiying,Bai, Yingguo,Zhou, Zhigang,Yao, Bin. 2009

[8]Two Family 11 Xylanases from Achaetomium sp Xz-8 with High Catalytic Efficiency and Application Potentials in the Brewing Industry. Zhao, Liang,Meng, Kun,Bai, Yingguo,Shi, Pengjun,Huang, Huoqing,Luo, Huiying,Wang, Yaru,Yang, Peilong,Yao, Bin,Zhao, Liang,Song, Wei. 2013

[9]Cloning of a New Xylanase Gene from Streptomyces sp TN119 Using a Modified Thermal Asymmetric Interlaced-PCR Specific for GC-Rich Genes and Biochemical Characterization. Zhou, Junpei,Huang, Huoqing,Meng, Kun,Shi, Pengjun,Wang, Yaru,Luo, Huiying,Yang, Peilong,Bai, Yingguo,Yao, Bin. 2010

[10]Gene Cloning, Overexpression, and Characterization of a Xylanase from Penicillium sp CGMCC 1669. Liu, Wanli,Shi, Pengjun,Yang, Peilong,Wang, Guozeng,Wang, Yaru,Luo, Huiying,Yao, Bin,Liu, Wanli,Chen, Qiang. 2010

[11]Purification and characterization of a xylanase from Bacillus subtilis isolated from the degumming line. Guo, Gang,Liu, Zhengchu,Xu, Junfei,Feng, Xiangyuan,Duan, Shengwen,Zheng, Ke,Cheng, Lifeng,Liu, Jianping,Dai, Xiaoyang,Xie, Daping,Peng, Keqing.

[12]Codon optimization of Bacillus licheniformis beta-1,3-1,4-glucanase gene and its expression in Pichia pastoris. Teng, Da,Fan, Ying,Yang, Ya-lin,Tian, Zi-gang,Luo, Jin,Wang, Jian-hua.

[13]The disruption of two salt bridges of the cold-active xylanase XynGR40 results in an increase in activity, but a decrease in thermostability. Wang, Guozeng,Wu, Jingjing,Lin, Juan,Ye, Xiuyun,Yao, Bin.

[14]A novel, alkali-tolerant thermostable xylanase from Saccharomonospora viridis: direct gene cloning, expression and enzyme characterization. Wang, Ziyuan,Jin, Yi,Wu, Huijun,Xie, Xiangming,Tian, Zhaofeng,Wu, Yuying.

[15]Utility of Thermostable Xylanases of Mycothermus thermophilus in Generating Prebiotic Xylooligosaccharides. Ma, Rui,Bai, Yingguo,Huang, Huoqing,Luo, Huiying,Yao, Bin,Ma, Rui,Fan, Yunliu,Ma, Rui,Chen, Sanfeng,Cai, Lei.

[16]Production of xylobiose from the autohydrolysis explosion liquor of corncob using Thermotoga maritima xylanase B (XynB) immobilized on nickel-chelated Eupergit C. Tan, S. S.,Li, D. Y.,Jiang, Z. Q.,Zhu, Y. P.,Shi, B.,Li, L. T..

[17]Improvement of alkali stability and thermostability of Paenibacillus campinasensis Family-11 xylanase by directed evolution and site-directed mutagenesis. Zheng, Hongchen,Liu, Yihan,Han, Yang,Lu, Fuping,Zheng, Hongchen,Liu, Yihan,Sun, Mingzhe,Han, Yang,Wang, Jianling,Lu, Fuping,Zheng, Hongchen,Sun, Junshe,Liu, Yihan,Wang, Jianling,Sun, Mingzhe,Lu, Fuping.

[18]Cloning of beta-1,3-1,4-glucanase gene from Bacillus licheniformis EGW039 (CGMCC 0635) and its expression in Escherichia coli BL21 (DE3). Teng, Da,Wang, Jian-Hua,Fan, Ying,Yang, Ya-lin,Tian, Zi-gang,Luo, Jin,Yang, Guan-pin,Zhang, Fan.

[19]A xylanase gene directly cloned from the genomic DNA of alkaline wastewater sludge showing application potential in the paper industry. Zhao, Yanyu,Luo, Huiying,Meng, Kun,Shi, Pengjun,Wang, Guozeng,Yuan, Tiezheng,Yao, Bin,Zhao, Yanyu,Yang, Peilong.

[20]Optimization of fermentation conditions for production of xylanase by a newly isolated strain, Penicillium thiersii ZH-19. Li, Yin,Cui, Fengjie,Liu, Zhiqiang,Ping, Lifeng,Xue, Yaping,Liu, Zhiqiang,Zhao, Hui,Ping, Lifeng,Yang, Yinan,Yan, Lijiao. 2009

作者其他论文 更多>>