文献类型: 外文期刊
第一作者: Wang, Xiaoyu
作者: Wang, Xiaoyu;Liu, Weina;Hou, Lingyu;Zheng, Fei;Xie, Xiangming;Wang, Xiaoyu;Luo, Huiying;Ma, Rui;You, Shuai;Zheng, Fei;Yao, Bin;Yu, Wangning
作者机构:
关键词: Gloeophyllum trabeum;Glycoside hydrolase family 10 (GH10);Xylanase;Thermo-tolerant
期刊名称:FOOD CHEMISTRY ( 影响因子:7.514; 五年影响因子:7.516 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: A xylanase gene of glycoside hydrolase family 10, GtXyn10, was cloned from Gloeophyllum trabeum CBS 900.73 and expressed in Pichia pastoris GS115. Purified recombinant GtXyn10 exhibited significant activities to xylan (100.0%), lichenan (11.2%), glucan (15.2%) and p-nitrophenol-beta-cellobiose (18.6%), demonstrated the maximum xylanase and glucanase activities at pH 4.5-5.0 and 75 degrees C, retained stability over the pH range of 2.0-7.5 and at 70 degrees C, and was resistant to pepsin and trypsin, most metal ions and SDS. Multiple sequence alignment and modeled-structure analysis identified a unique Gly48 in GtXyn10, and site-directed mutagenesis of Gly48 to Lys improved the temperature optimum up to 80 degrees C. Under simulated mashing conditions, GtXyn10 (80 U) reduced the mash viscosity by 12.8% and improved the filtration rate by 31.3%. All these properties above make GtXyn10 attractive for potential applications in the feed and brewing industries. (C) 2015 Elsevier Ltd. All rights reserved.
分类号: TS2`TS201.2
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