The effect of heat stress on gene expression and synthesis of heat-shock and milk proteins in bovine mammary epithelial cells

文献类型： 外文期刊

第一作者： Hu, Han

作者： Hu, Han;Zhang, Yangdong;Zheng, Nan;Cheng, Jianbo;Wang, Jiaqi;Hu, Han;Zhang, Yangdong;Zheng, Nan;Cheng, Jianbo;Wang, Jiaqi;Hu, Han;Zhang, Yangdong;Zheng, Nan;Cheng, Jianbo;Wang, Jiaqi

作者机构：

关键词： casein;heat shock protein;heat stress;mammary epithelial cell

期刊名称：ANIMAL SCIENCE JOURNAL （ 影响因子：1.749； 五年影响因子：1.909 ）

ISSN：

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收录情况： SCI

摘要： In this study, bovine mammary epithelial cells were used to study stress responses after cells were exposed to 42 degrees C for 0.5, 1, 3, 5, 8 or 12 h, and 38 degrees C as control. The transcription of the genes (HSP27, HSP70 and HSP90) of heat shock protein (Hsp) was significantly enhanced under heat stress (HS). The peak transcription of HSP70 was 14 times the control at 1 h. Expression of proteins Hsp27 and Hsp70 was gradually increased under HS, with rapid deposition of Hsp70 in epithelial cells. The major milk protein genes of beta-casein (CSN2) and butyrophilin (BTN1A1) were down-regulated and the synthesis of total caseins was decreased. After the cells were under HS (42 degrees C) for 1 or 5 h, the cells were cultured at 38 degrees C for 1, 6, 12 or 24 h for recovery. When the cells were cultured at 38 degrees C for 24 h after HS for 1 h, the transcription of HSP70, HSP90, CSN2 and BTN reached normal levels. Our results suggest that HS initiated Hsp synthesis and decreased the milk protein synthesis. Hsp70 is extremely sensitive to HS and mainly responsible for mammary cell protection from HS.

分类号： S8

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