Rapid differentiation of Ralstonia solanacearum avirulent and virulent strains by cell fractioning of an isolate using high performance liquid chromatography

文献类型： 外文期刊

第一作者： Zheng, Xuefang

作者： Zheng, Xuefang;Lin, Naiquan;Zheng, Xuefang;Zhu, Yujing;Liu, Bo;Yu, Qian

作者机构：

关键词： Avirulent;Bacterial wilt disease;High performance liquid chromatography;Ralstonia solanacearum;Virulent strain

期刊名称：MICROBIAL PATHOGENESIS （ 影响因子：3.738； 五年影响因子：3.663 ）

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收录情况： SCI

摘要： Ralstonia solanacearum is one of the most destructive plant bacterial pathogens worldwide. The population dynamics and genetic stability are important issues, especially when an avirulent strain is used for biocontrol. In this study, we developed a rapid method to differentiate the virulent and avirulent strains of R. solanacearum and to predict the biocontrol efficiency of an avirulent strain using high performance liquid chromatography (HPLC). Three chromatographic peaks P-1, P-2 and P-3 were observed on the HPLC spectra among 68 avirulent and 28 virulent R. solanacearum strains. Based on the HPLC peaks, 96 strains total were assigned to three categories. For avirulent strains, the intense peak is P1, while for virulent strains, P3 is the majority. Based on the HLPC spectra of R. solanacearum strains, a chromatography titer index (CTI) was established as CTi; = S-i;/(S-1+S-2+S-3) x 100% (i represents an individual HPLC peak; S-1, S-2 and S-3 represent peak areas of P-1, P-2 and P-3, respectively). The avirulent strains had high values of C-Tl-1 ranging from 63.6 to 100.0%, while the virulent strains displayed high values of CTI3 ranging from 90.2 to 100.0%. Biological inoculation studies of 68 avirulent strains revealed that the biocontrol efficacy was the best when CTI1 =100%. The purity and genetic stability of R. solanacearum strains were confirmed in the P-1 fraction of avirulent strain FJAT-1957 and P-3 fraction of virulent strain FJAT-1925 after 30 generations of consecutive subculture. These results confirmed that fractioning by HPLC and their deduced CTI can be used for rapid and efficient evaluation and prediction of an isolate of R. solanacearum. To the best of our knowledge, this is the first report that HPLC fractioning can be used for rapid differentiation of virulent and avirulent strains of R. solanacearum. (C) 2015 Elsevier Ltd. All rights reserved.

分类号： R3

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