Effect of Nonstructural Protein 2 Hypervariable Regions in the Replication of Porcine Reproductive and Respiratory Syndrome Virus in Marc-145 Cells
文献类型: 外文期刊
第一作者: Liu, Ying
作者: Liu, Ying;Wang, Feng-Xue;Wen, Yang -Jun;Li, Zhen-Guang;Liu, Xing;Sun, Na;Yang, Yong;Zhang, Shu-Qin;Zhu, Hong-Wei;Cheng, Shi-Peng;Wu, Hua
作者机构:
关键词: Nonstructural protein 2;Transiently expressed amino acids;Truncated polypeptides;Porcine reproductive and respiratory syndrome virus;Viral replication
期刊名称:INTERVIROLOGY ( 影响因子:1.763; 五年影响因子:1.929 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: Background: Highly pathogenic (HP) porcine reproductive and respiratory syndrome virus (PRRSV) causes prolonged high fever, red discoloration of the body, blue ears and a high mortality. Previously, we found that the PRRSV vaccine strain TJM contained a deletion of 120 amino acids (aa 628-747) in nonstructural protein 2 (Nsp2). We aimed to explore the replication features of PRRSV after adding the transiently expressed product of these 120 aa in vitro. Methods: We constructed seven eukaryotic expression plasnnids containing different parts of the 120-aa sequence, transfected them into Marc-145 cells and then inoculated the cells with 103 TCID50 TJM per well. We detected virus replication at mRNA and protein level by real-time RT-PCR and Western blotting, respectively, and determined the virus titer. Results: The transiently expressed 120 aa and one of its truncated poly peptides inhibited PRRSV TJM propagation on Marc-145 cells. The complete 120-aa sequence induced a remarkable decrease in PRRSV replication, causing a reduction in structural protein levels between 36 and 48 h after infection. Additionally, aa 628-727 partly reduced the replication of PRRSV on Marc-145 cells. Conclusions: The 120 aa from Nsp2, especially aa 628-727, play a negative role in PRRSV TJM proliferation. (C) 2015 S. Karger AG, Basel
分类号: Q93
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