Effect of Nonstructural Protein 2 Hypervariable Regions in the Replication of Porcine Reproductive and Respiratory Syndrome Virus in Marc-145 Cells

文献类型： 外文期刊

第一作者： Liu, Ying

作者： Liu, Ying;Wang, Feng-Xue;Wen, Yang -Jun;Li, Zhen-Guang;Liu, Xing;Sun, Na;Yang, Yong;Zhang, Shu-Qin;Zhu, Hong-Wei;Cheng, Shi-Peng;Wu, Hua

作者机构：

关键词： Nonstructural protein 2;Transiently expressed amino acids;Truncated polypeptides;Porcine reproductive and respiratory syndrome virus;Viral replication

期刊名称：INTERVIROLOGY （ 影响因子：1.763； 五年影响因子：1.929 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Background: Highly pathogenic (HP) porcine reproductive and respiratory syndrome virus (PRRSV) causes prolonged high fever, red discoloration of the body, blue ears and a high mortality. Previously, we found that the PRRSV vaccine strain TJM contained a deletion of 120 amino acids (aa 628-747) in nonstructural protein 2 (Nsp2). We aimed to explore the replication features of PRRSV after adding the transiently expressed product of these 120 aa in vitro. Methods: We constructed seven eukaryotic expression plasnnids containing different parts of the 120-aa sequence, transfected them into Marc-145 cells and then inoculated the cells with 103 TCID50 TJM per well. We detected virus replication at mRNA and protein level by real-time RT-PCR and Western blotting, respectively, and determined the virus titer. Results: The transiently expressed 120 aa and one of its truncated poly peptides inhibited PRRSV TJM propagation on Marc-145 cells. The complete 120-aa sequence induced a remarkable decrease in PRRSV replication, causing a reduction in structural protein levels between 36 and 48 h after infection. Additionally, aa 628-727 partly reduced the replication of PRRSV on Marc-145 cells. Conclusions: The 120 aa from Nsp2, especially aa 628-727, play a negative role in PRRSV TJM proliferation. (C) 2015 S. Karger AG, Basel

分类号： Q93

  • 相关文献

[1]Isolation and Sequence Analysis of Highly Pathogenic Porcine reproductive and respiratory syndrome virus from Swine Herds in the Jilin Province of China. Wang, Feng-Xue,Guo, Li,Yang, Yan-Ling,Song, Ni,Chen, Li-Zhi,Cheng, Shi-Peng,Wen, Yong-Jun. 2013

[2]Porcine alveolar macrophage polarization is involved in inhibition of porcine reproductive and respiratory syndrome virus (PRRSV) replication. Wang, Longtao,Li, Yiru,Hu, Shouping,Liu, Qiang,Li, Yiru,Xu, Lu,Zhang, Zhuo,Cai, Xuehui,He, Xijun,Xu, Lu. 2017

[3]A single amino acid in the C-terminus of VP3 protein influences the replication of attenuated infectious bursal disease virus in vitro and in vivo. Wang, Yongqiang,Qi, Xiaole,Kang, Zhonghui,Yu, Fei,Qin, Liting,Gao, Honglei,Gao, Yulong,Wang, Xiaomei. 2010

[4]GADD45 beta, an anti-tumor gene, inhibits avian leukosis virus subgroup J replication in chickens. Zhang, Xinheng,Yan, Zhuanqiang,Li, Xinjian,Lin, Wencheng,Dai, Zhenkai,Yan, Yiming,Lu, Piaopiao,Chen, Weiguo,Chen, Feng,Ma, Jingyun,Xie, Qingmei,Zhang, Xinheng,Yan, Zhuanqiang,Li, Xinjian,Lin, Wencheng,Dai, Zhenkai,Yan, Yiming,Lu, Piaopiao,Chen, Weiguo,Chen, Feng,Ma, Jingyun,Xie, Qingmei,Zhang, Xinheng,Yan, Zhuanqiang,Li, Xinjian,Lin, Wencheng,Dai, Zhenkai,Yan, Yiming,Lu, Piaopiao,Chen, Weiguo,Chen, Feng,Ma, Jingyun,Xie, Qingmei,Zhang, Xinheng,Li, Xinjian,Lin, Wencheng,Dai, Zhenkai,Yan, Yiming,Lu, Piaopiao,Chen, Weiguo,Chen, Feng,Ma, Jingyun,Xie, Qingmei,Lin, Wencheng,Chen, Weiguo,Chen, Feng,Xie, Qingmei,Zhang, Huanmin. 2016

[5]Inhibition of the replication of grass carp reovirus in CIK cells with plasmid-transcribed shRNAs. Zeng, Lingbing,Ma, Jie,Wang, Weiming,Zeng, Lingbing.

[6]The down-regulation of casein kinase 1 alpha as a host defense response against infectious bursal disease virus infection. Zhang, Lizhou,Li, Hui,Chen, Yuming,Gao, Xiang,Lu, Zhen,Gao, Li,Wang, Yongqiang,Gao, Yulong,Gao, Honglei,Liu, Changjun,Cui, Hongyu,Zhang, Yanping,Pan, Qing,Qi, Xiaole,Wang, Xiaomei,Wang, Xiaomei.

[7]Influenza a virus NS1 protein induced A20 contributes to viral replication by suppressing interferon-induced antiviral response. Feng, Wenjing,Sun, Xiaoning,Zhang, Maolin,Guan, Zhenhong,Duan, Ming,Shi, Ning.

[8]Heat shock protein 70 is necessary for Rice stripe virus infection in plants. Jiang, Shanshan,Li, Kunfeng,Lin, Lin,Zheng, Hongying,Chen, Jianping,Jiang, Shanshan,Lu, Yuwen,Li, Kunfeng,Yan, Fei,Chen, Jianping.

[9]Chicken galectin-1B inhibits Newcastle disease virus adsorption and replication through binding to hemagglutinin-neuraminidase (HN) glycoprotein. Sun, Junfeng,Han, Zongxi,Qi, Tianming,Zhao, Ran,Liu, Shengwang. 2017

[10]Long Non-Coding RNAs: emerging and versatile Regulators in Host-virus interactions. Meng, Xing-Yu,Luo, Yuzi,Anwar, Muhammad Naveed,Sun, Yuan,Gao, Yao,Zhang, Huawei,Qiu, Hua-Ji,Munir, Muhammad. 2017

[11]Porcine serum amyloid A3 is expressed in extrahepatic tissues and facilitates viral replication during porcine respiratory and reproductive syndrome virus infection. Huan, Beili,Liu, Ke,Li, Yuming,Wei, Jianchao,Shao, Donghua,Shi, Yuanyuan,Qiu, Yafeng,Li, Beibei,Ma, Zhiyong,Li, Beibei. 2018

[12]Atypical Classical Swine Fever Infection Changes Interleukin Gene Expression in Pigs. Sun, Y. K.,Sun, Y. K.,Zhang, X. M.,Du, M.,Li, Y. X.,Pan, H. B.,Yan, Y. L.,Yang, Y. A..

[13]Eukaryotic translational initiation factor 4AII reduces the replication of infectious bursal disease virus by inhibiting VP1 polymerase activity. Gao, Li,Li, Kai,Zhong, Li,Zhang, Lizhou,Qi, Xiaole,Wang, Yongqiang,Gao, Yulong,Wang, Xiaomei.

[14]Integrin v1 Modulation Affects Subtype B Avian Metapneumovirus Fusion Protein-mediated Cell-Cell Fusion and Virus Infection. Yun, Bing-Ling,Guan, Xiao-Lu,Liu, Yong-Zhen,Zhang, Yao,Wang, Yong-Qiang,Qi, Xiao-Le,Cui, Hong-Yu,Liu, Chang-Jun,Zhang, Yan-Ping,Gao, Hong-Lei,Gao, Li,Li, Kai,Gao, Yu-Long,Wang, Xiao-Mei,Wang, Xiao-Mei.

[15]Annexin A2 (ANXA2) interacts with nonstructural protein 1 and promotes the replication of highly pathogenic H5N1 avian influenza virus. Ma, Yong,Sun, Jiashan,Gu, Linlin,Bao, Hongmei,Zhao, Yuhui,Tian, Guobin,Wang, Xiurong,Chen, Hualan,Shi, Lin,Yao, Wei. 2017

[16]LiCl inhibits PRRSV infection by enhancing Wnt/beta-catenin pathway and suppressing inflammatory responses. Hao, Hong-ping,Li, Jia-rong,Wang, Yue,Ni, Bo,Wang, Rui,Wang, Xin,Sun, Ming-xia,Fan, Hong-jie,Mao, Xiang,Wen, Li-bin,Fan, Hong-jie. 2015

[17]Genomic analysis of a recombinant NADC30-like porcine reproductive and respiratory syndrome virus in china. Wang, Lin-jian,Guo, Zhenhua,Qiao, Songlin,Li, Rui,Xie, Sha,Chen, Xin-xin,Zhang, Gaiping,Wang, Lin-jian,Zhang, Gaiping,Wan, Bo,Zhang, Gaiping. 2018

[18]Mechanisms of suppression of interferon production by porcine reproductive and respiratory syndrome virus. Zhu, L.,Zhou, Y.,Tong, G.. 2012

[19]Porcine 2 ', 5 '-oligoadenylate synthetase 2 inhibits porcine reproductive and respiratory syndrome virus replication in vitro. Zhao, Mengmeng,Wan, Bo,He, Jian,Zhang, Gaiping,Li, Huawei,Chen, Xinxin,Wang, Linjian,Wang, Yinbiao,Xie, Sha,Qiao, Songlin,Zhang, Gaiping,Zhang, Gaiping. 2017

[20]Development and validation of reverse transcription loop-mediated isothermal amplification for detection of PRRSV. Chen, Changmu,Cui, Shangjin,Zhang, Chaofan,Li, Jun,Wang, Jinbao. 2010

作者其他论文 更多>>

微信小程序