Enzymatic saccharification of high pressure assist-alkali pretreated cotton stalk and structural characterization

文献类型： 外文期刊

第一作者： Shuang-kui Du

作者： Shuang-kui Du;Xia Su;Weihua Yang;Yanqin Wang;Meng Kuang;Lei Ma;Dan Fang;Dayun Zhou

作者机构：

关键词： Cotton stalk;High pressure assist-alkali pretreatment (HPAP);Enzymatic saccharification;Structural characterization;RSM

期刊名称：CARBOHYDRATE POLYMERS （ 影响因子：9.381； 五年影响因子：8.678 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Cotton stalk is a potential biomass for bioethanol production, while the conversion of direct saccharification or biotransformation of cotton stalk is extremely low due to the recalcitrant nature of lignocellulose. To enhance the enzymatic conversion of cotton stalks, the enzymatic saccharification parameters of high pressure assist -alkali pretreatment (HPAP) cotton stalk were optimized in the present study. Results indicated that a maximum reducing sugar yield of 54.7 g/100 g dry biomass cellulose was achieved at a substrate concentration of 2%, 100 rpm agitation, 0.6 g/g enzyme loading, 40 degrees C hydrolysis temperature, 50 h saccharification time, and pH 5.0. Scanning electron microscopy, X-ray diffraction, and Fourier transform infrared spectroscopy were used to identify structural changes in native, pretreated biomass and hydrolyzed residues. Structural analysis revealed large part of amorphous cellulose and partial crystalline cellulose in the HPAP cotton stalk were hydrolyzed during enzymatic treatment. HPAP cotton stalk can be used as a potential feed stock for bioethanol production. (C) 2015 Elsevier Ltd. All rights reserved.

分类号： O629`Q53

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