Genetic polymorphisms and DNA methylation in exon 1 CpG-rich regions of PACAP gene and its effect on mRNA expression and growth traits in half smooth tongue sole (Cynoglossus semilaevis)

文献类型： 外文期刊

第一作者： Si, Yufeng

作者： Si, Yufeng;He, Feng;Wen, Haishen;Li, Jifang;Zhao, Junli;Ren, Yuanyuan;Zhao, Meilin;Ji, Liqin;Huang, Zhengju;Zhang, Mo;Si, Yufeng;He, Feng;Wen, Haishen;Li, Jifang;Zhao, Junli;Ren, Yuanyuan;Zhao, Meilin;Ji, Liqin;Huang, Zhengju;Zhang, Mo;Chen, Songlin

作者机构：

关键词： PACAP;SNP;DNA methylation;Growth;Molecular markers

期刊名称：FISH PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：2.794； 五年影响因子：2.876 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： The pituitary adenylate cyclase activating polypeptide (PACAP) is a new type of hypophysiotropic hormone and plays an important role in regulating the synthesis and secretion of growth hormone and gonadotropin. The research on the relationship between PACAP and different growth traits would contribute to explain its function during the process of growth. Moreover, epigenetic modifications, especially DNA methylation at the CpG sites of the SNPs, play important roles in regulating gene expression. The results suggest that a SNP mutation (c.C151G) in the PACAP gene of male half smooth tongue sole (Cynoglossus semilaevis) is significantly associated with growth traits and serum physiological and biochemical parameters such as inorganic phosphorus (P < 0.05). The SNP is located in a CpG-rich region of exon 1. Intriguingly, the transition (C -> G) added a new methylation site of PACAP gene. This SNP was also significantly related to the expression and methylation level of PACAP (P < 0.05). Individuals with GG genotype had faster growth rates than those of CG and CC genotypes. Moreover, GG genotype had significantly higher PACAP expression level and lower methylation level than CG and CC genotypes. In the serum indexes, only inorganic phosphorus content within GG genotypes was significantly higher than CC genotypes. This implied that the mutation and methylation status of PACAP gene could influence growth traits and this locus could be considered as a candidate genetic or epigenetic marker for Cynoglossus semilaevis molecular breeding.

分类号： S9

  • 相关文献

[1]Association of pituitary adenylate cyclase-activating polypeptide and myogenic factor 6 genes with growth traits in Nile tilapia (Oreochromis niloticus). Zou, Guiwei,Zhu, Yuanyuan,Liang, Hongwei,Li, Zhong,Zhu, Yuanyuan.

[2]Growth differences and differential expression analysis of pituitary adenylate cyclase activating polypeptide (PACAP) and growth hormone-releasing hormone (GHRH) between the sexes in half-smooth tongue sole Cynoglossus semilaevis. Ji, Xiang-Shan,Chen, Song-Lin,Xu, Tian-Jun,Yang, Jing-Feng,Tian, Yong-Sheng,Ji, Xiang-Shan,Jiang, Yun-Liang. 2011

[3]Identification of a new stripe rust resistance gene in Chinese winter wheat Zhongmai 175. Lu Jia-ling,Chen Can,Liu Peng,He Zhong-hu,Xia Xian-chun,Chen Can,He Zhong-hu. 2016

[4]Rapid alterations of gene expression and cytosine methylation in newly synthesized Brassica napus allopolyploids. Xu, Yanhao,Zhong, Lan,Wang, Jianbo,Wu, Xiaoming,Fang, Xiaoping.

[5]Genomic structure, polymorphism and expression analysis of growth hormone-releasing hormone and pituitary adenylate cyclase activating polypeptide genes in the half-smooth tongue sole (Cynoglossus semilaevis). Ma, Q.,Liu, S. F.,Zhuang, Z. M.,Lin, L.,Sun, Z. Z.,Liu, C. L.,Tang, Q. S.,Ma, Q.,Lin, L.,Su, Y. Q.. 2011

[6]Demographic analysis based on the growth parameter of sharks. Chen, PM,Yuan, WW.

[7]Genome-Wide Discovery of Microsatellite Markers from Diploid Progenitor Species, Arachis duranensis and A. ipaensis, and Their Application in Cultivated Peanut (A. hypogaea). Zhao, Chuanzhi,Qiu, Jingjing,Wang, Jiangshan,Ren, Xuezhen,Xia, Han,Wan, Shubo,Wang, Xingjun,Zhao, Chuanzhi,Qiu, Jingjing,Wang, Jiangshan,Ren, Xuezhen,Xia, Han,Wan, Shubo,Wang, Xingjun,Qiu, Jingjing,Ma, Changle,Wang, Xingjun,Agarwal, Gaurav,Guo, Baozhu,Agarwal, Gaurav,Varshney, Rajeev K.,Pandey, Manish K.,Bertioli, David J.. 2017

[8]Phylogenetic analysis and rapid identification of the whitefly, Bemisia afer, in China. Chu, Dong,Liu, Guoxia,Tao, Yunli,Wan, Fanghao,Gill, Ray J.. 2010

[9]Identification of expressed resistance gene-like sequences by data mining in 454-derived transcriptomic sequences of common bean (Phaseolus vulgaris L.). Liu, Zhanji,Todd, Antonette,Kalavacharla, Venu,Liu, Zhanji,Crampton, Mollee,Kalavacharla, Venu,Liu, Zhanji,Crampton, Mollee. 2012

[10]Molecular detection of high- and low-molecular-weight glutenin subunit genes in common wheat cultivars from 20 countries using allele-specific markers. H. Jin,J. Yan,R. J. Peña,X. C. Xia,A. Morgounov,L. M. Han,Y. Zhang,Z. H. He. 2011

[11]Development of a novel and efficient strategy for practical identification of Pyrus spp (Rosaceae) cultivars using RAPD fingerprints. Lin, J.,Chang, Y. H.,Wang, X. C.,Fang, J. G.,Wang, X. C.,Fang, J. G.. 2011

[12]Achievements and prospects of genomics-assisted breeding in three legume crops of the semi-arid tropics. Varshney, Rajeev K.,Mohan, S. Murali,Gaur, Pooran M.,Pandey, Manish K.,Sawargaonkar, Shrikant L.,Chitikineni, Annapurna,Janila, Pasupuleti,Saxena, K. B.,Sharma, Mamta,Rathore, Abhishek,Mallikarjuna, Nalini,Gowda, C. L. L.,Varshney, Rajeev K.,Varshney, Rajeev K.,Varshney, Rajeev K.,Liang, Xuanqiang,Gangarao, N. V. P. R.,Pandey, Manish K.,Bohra, Abhishek,Pratap, Aditya,Datta, Subhojit,Chaturvedi, S. K.,Nadarajan, N.,Kimurto, Paul K.,Fikre, Asnake,Tripathi, Shailesh,Bharadwaj, Ch.,Anuradha, G.,Babbar, Anita,Choudhary, Arbind K.,Mhase, M. B.,Mannur, D. M.. 2013

[13]Efficient identification of ornamental peach cultivars using RAPD markers with a manual cultivar identification diagram strategy. Han, J.,Wang, W. Y.,Leng, X. P.,Jiang, W. B.,Guo, L.,Yu, M. L.,Ma, R. J.. 2014

[14]An improved strategy based on RAPD markers efficiently identified 95 peach cultivars. Yu, M. L.,Ma, R. J.,Shen, Z. J.,Wang, W. Y.,Fang, G.,Wang, W. Y.,Fang, G.. 2012

[15]Molecular and Cytogenetic Characterization of New Wheat-Dasypyrum breviaristatum Derivatives with Post-Harvest Re-Growth Habit. Li, Guangrong,Li, Donghai,Gao, Dan,Zhang, Jie,Yang, Zujun,Yang, Ennian. 2015

[16]A new strategy employed for identification of sweet orange cultivars with RAPD markers. Sun, X.,Mu, Q.,Wang, C.,Wang, X. C.,Fang, J. G.,Jiang, D.. 2012

[17]Identification and characterization of a RAPD/SCAR marker linked to a resistance gene for soybean mosaic virus in soybean. Zheng, CM,Chang, RZ,Qiu, LJ,Chen, PY,Wu, X,Chen, SY. 2003

[18]Identification of QTL for growth- and grain yield-related traits in rice across nine locations of Asia. Hittalmani, S,Huang, N,Courtois, B,Venuprasad, R,Shashidhar, HE,Zhuang, JY,Zheng, KL,Liu, GF,Wang, GC,Sidhu, JS,Srivantaneeyakul, S,Singh, VP,Bagali, PG,Prasanna, HC,McLaren, G,Khush, GS. 2003

[19]Verification and evaluation of grain QTLs using RILs from TD70 x Kasalath in rice. Zhang, Y. D.,Liang, Y. L.,Wang, C. L.,Zhang, Y. D.,Zheng, J.,Wang, C. L.,Liang, Z. K.,Peng, Z. H.. 2015

[20]Trail of decryption of molecular research on Botryosphaeriaceae in woody plants. Chethana, K. W. Thilini,Li, Xinghong,Zhang, Wei,Yan, Jiye,Chethana, K. W. Thilini,Hyde, Kevin D.,Chethana, K. W. Thilini,Hyde, Kevin D.. 2016

作者其他论文 更多>>

微信小程序