Thapsigargin induces apoptosis when autophagy is inhibited in HepG2 cells and both processes are regulated by ROS-dependent pathway

文献类型： 外文期刊

第一作者： Wang, Congcong

作者： Wang, Congcong;Tang, Shusheng;Zhao, Dongxu;Zhang, Chaoming;Zhang, Shen;Deng, Sijun;Zhou, Yan;Xiao, Xilong;Wang, Congcong;Li, Tao

作者机构：

关键词： Apoptosis;Autophagy;Thapsigargin;ROS;HepG2 cells

期刊名称：ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY （ 影响因子：4.86； 五年影响因子：4.462 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Thapsigargin (TG), is widely used to induce endoplasmic reticular stress. Treated with TG for a long time, cells suffer the unfolded protein response (UPR) to elude apoptosis, but may activate autophagy. However, the switch between autophagy and apoptosis is unclear. To clarify the key signal for selection of these two protective responses, we studied the correlation of autophagy and apoptosis in HepG2 cells exposed to TG with time. TG induced apoptosis in HepG2 cells was evidenced by typical cell morphological changes and the activation of caspase-12, caspase-9 and caspase-3. Meanwhile, cytochrome c was released following with the dissipation of mitochondrial membrane potential (MMP), and the ratio of Bax/Bcl-2 was increased. TG-induced autophagy was confirmed by the accumulation of MDC, GFP-LC3 staining autophagic vacuoles, and the improved expression of LC3 II and Beclin-1. Additionally, inhibited autophagy via chloroquine (CQ) markedly enhanced the apoptosis induced by TG, which was linked to the Bcl-2 family. Furthermore, TG induced the generation of reactive oxygen species (ROS), and the ROS scavenger effectively suppressed TG-induced apoptosis and autophagy. All these results proved that restraint of autophagy may enhance TG-induced apoptosis through increasing the Bax/Bcl-2 ratio and both processes were regulated by ROS. (C) 2015 Elsevier B.V. All rights reserved.

分类号： R9

  • 相关文献

[1]Cytotoxic effects and apoptosis induction of enrofloxacin in hepatic cell line of grass carp (Ctenopharyngodon idellus). Liu, Bo,Ge, Xianping,Xie, Jun,Xu, Pao,Liu, Bo,Cui, Yanting,Ge, Xianping,Xie, Jun,Xu, Pao,Brown, Paul B..

[2]Apoptosis inducing and differentiation enhancement effect of oridonin on the all-trans-retinoic acid-sensitive and -resistant acute promyelocytic leukemia cells. Wu, Y.,Gao, F.,Yang, P.,Fang, Y.,Tang, Q.,Li, W.. 2010

[3]Fatty acid synthase knockout impairs early embryonic development via induction of endoplasmic reticulum stress in pigs. Guo, Jing,Niu, Ying-Jie,Shin, Kyung-Tae,Kwon, Jeong-Woo,Kim, Nam-Hyung,Cui, Xiang-Shun,Guo, Jing. 2018

[4]The effect of emodin on cytotoxicity, apoptosis and antioxidant capacity in the hepatic cells of grass carp (Ctenopharyngodon idellus). Cui, Yan-ting,Liu, Bo,Xie, Jun,Xu, Pao,Habte-Tsion, H. -Michael,Zhang, Yuan-yuan,Cui, Yan-ting,Liu, Bo,Xie, Jun,Xu, Pao,Habte-Tsion, H. -Michael,Zhang, Yuan-yuan.

[5]p38 MAPK Signaling Mediates Mitochondrial Apoptosis in Cancer Cells Induced by Oleanolic Acid. Liu, Jia,Wu, Ning,Liu, Ge,Lin, Xiu-Kun,Zheng, Lan-Hong,Ma, Lei-Na,Liu, Ge,Lin, Xiu-Kun,Zhong, Jia-Teng. 2014

[6]Mitophagy promotes replication of oncolytic Newcastle disease virus by blocking intrinsic apoptosis in lung cancer cells. Meng, Gang,Xia, Mao,Wang, Diancheng,Chen, Aiping,Wang, Hongwei,Yu, Decai,Wei, Jiwu,Yu, Decai,Wei, Jiwu,Wang, Yongshan. 2014

[7]3 '-epi-12 beta-hydroxyfroside, a new cardenolide, induces cytoprotective autophagy via blocking the Hsp90/Akt/mTOR axis in lung cancer cells. Sun, Yan,Huang, Yong-Hao,Huang, Feng-Ying,Wang, Cai-Chun,Lin, Ying-Ying,Huang, Canhua,Li, Yue-Nan,Tan, Guang-Hong,Sun, Yan,Huang, Yong-Hao,Huang, Feng-Ying,Wang, Cai-Chun,Lin, Ying-Ying,Huang, Canhua,Li, Yue-Nan,Tan, Guang-Hong,Mei, Wen-Li,Dai, Hao-Fu,Liu, Quan,Liu, Quan,Huang, Canhua,Li, Yue-Nan. 2018

[8]Caspase-1 participates in apoptosis of salivary glands in Rhipicephalus haemaphysaloides. Yu, Xinmao,Zhou, Yongzhi,Cao, Jie,Zhang, Houshuang,Gong, Haiyan,Zhou, Jinlin,Zhou, Jinlin. 2017

[9]Programmed Cell Death in the Honey Bee (Apis mellifera) (Hymenoptera: Apidae) Worker Brain Induced by Imidacloprid. Wu, Yan-Yan,Zhou, Ting,Wang, Qiang,Dai, Ping-Li,Xu, Shu-Fa,Jia, Hui-Ru,Wang, Xing.

[10]Autophagy in Lepidoptera: more than old wine in new bottle. Tettamanti, G.,Grimaldi, A.,de Eguileor, M.,Cao, Y.,Feng, Q.. 2011

[11]Toxicarioside O induces protective autophagy in a sirtuin-1-dependent manner in colorectal cancer cells. Huang, Yong-Hao,Sun, Yan,Huang, Feng-Ying,Li, Yue-Nan,Wang, Cai-Chun,Tan, Guang-Hong,Huang, Canhua,Huang, Yong-Hao,Sun, Yan,Huang, Feng-Ying,Li, Yue-Nan,Wang, Cai-Chun,Tan, Guang-Hong,Huang, Canhua,Mei, Wen-Li,Dai, Hao-Fu,Huang, Canhua,Huang, Canhua. 2017

[12]BmATG5 and BmATG6 mediate apoptosis following autophagy induced by 20-hydroxyecdysone or starvation. Xie, Kun,Tian, Ling,Guo, Xinyu,Li, Kang,Li, Jianping,Deng, Xiaojuan,Zhong, Yangjin,Huang, Zhijun,Liu, Jiping,Yang, Wanying,Cao, Yang,Xie, Kun,Tian, Ling,Li, Kang,Li, Sheng,Li, Qingrong,Xia, Qingyou.

[13]Autophagy precedes apoptosis during the remodeling of silkworm larval midgut. Franzetti, Eleonora,Grimaldi, Annalisa,Tettamanti, Gianluca,Huang, Zhi-Jun,Shi, Yan-Xia,Xie, Kun,Deng, Xiao-Juan,Li, Jian-Ping,Li, Qing-Rong,Yang, Wan-Ying,Zeng, Wen-Nian,Cao, Yang,Li, Qing-Rong,Casartelli, Morena,Deng, Hui-Min,Feng, Qili,Cappellozza, Silvia,Xia, Qingyou.

[14]The Interactions between ZnO Nanoparticles (NPs) and alpha-Linolenic Acid (LNA) Complexed to BSA Did Not Influence the Toxicity of ZnO NPs on HepG2 Cells. Zhou, Yiwei,Fang, Xin,Xiao, Aiping,Liu, Liangliang,Cao, Yi,Zhou, Yiwei,Fang, Xin,Gong, Yu,Xie, Yixi,Cao, Yi. 2017

[15]The presence of oleate stabilized ZnO nanoparticles (NPs) and reduced the toxicity of aged NPs to Caco-2 and HepG2 cells. Fang, Xin,Jiang, Leying,Gong, Yu,Li, Juan,Cao, Yi,Liu, Liangliang,Cao, Yi.

[16]Histological changes and differences in activities of some antioxidant enzymes and hydrogen peroxide content during somatic embryogenesis of Musa AAA cv. Yueyoukang 1. Jiang, Shuang,Chen, Houbin,Xu, Chunxiang,Xie, Lijun,Li, Huaping,Lin, Guimei,Takac, Tomas,Samaj, Jozef,Samaj, Jozef. 2012

[17]Dose dependent rhizospheric Ni toxicity evaluation: Membrane stability and antioxidant potential of Vigna species. Mahmood, Seema,Ishtiaq, Shabnam,Yasin, Ghulam,Irshad, Ahsan. 2016

[18]TIP60 contributes to porcine embryonic development by regulating DNA damage response. Guo, Jing,Zhou, Wenjun,Niu, Ying-Jie,Shin, Kyung-Tae,Kim, Nam-Hyung,Cui, Xiang-Shun,Guo, Jing,Heo, Young Tae. 2018

[19]4-Coumarate-CoA Ligase-Like Gene OsAAE3 Negatively Mediates the Rice Blast Resistance, Floret Development and Lignin Biosynthesis. Liu, Hao,Gu, Fengwei,Dong, Shuangyu,Liu, Wei,Huang, Ming,Xiao, Wuming,Yang, Guili,Liu, Yongzhu,Guo, Tao,Wang, Hui,Wang, Jiafeng,Chen, Zhiqiang,Guo, Zhenhua,Ke, Shanwen,Sun, Dayuan. 2017

[20]Strand-specific RNA-Seq transcriptome analysis of genotypes with and without low-phosphorus tolerance provides novel insights into phosphorus-use efficiency in maize. Du, Qingguo,Wang, Kai,Xu, Cheng,Zou, Cheng,Xie, Chuanxiao,Xu, Yunbi,Li, Wen-Xue. 2016

作者其他论文 更多>>

微信小程序