Enzymatic modification of chitosan by cinnamic acids: Antibacterial activity against Ralstonia solanacearum

文献类型: 外文期刊

第一作者: Yang, Caifeng

作者: Yang, Caifeng;Zhou, Yu;Zheng, Yu;Li, Changlong;Sheng, Sheng;Wang, Jun;Wu, Fuan;Yang, Caifeng;Zhou, Yu;Zheng, Yu;Li, Changlong;Sheng, Sheng;Wang, Jun;Wu, Fuan

作者机构:

关键词: Cinnamic acids;Chitosan derivatives;Antibacterial activity

期刊名称:INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES ( 影响因子:6.953; 五年影响因子:6.737 )

ISSN:

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收录情况: SCI

摘要: This study aimed to identify chitosan polymers that have antibacterial activity against the bacterial wilt pathogen. The chitosan polymers were enzymatically synthesized using chitosan and five cinnamic acids (CADS): caffeic acid (CA), ferulic acid (FA), cinnamic acid (CIA), p-coumaric acid (COA) and chlorogenic acid (CHA), using laccase from Pleurotus ostreatus as a catalyst. The reaction was performed in a phosphate buffered solution under heterogenous reaction conditions. The chitosan derivatives (CTS-g-CADs) were characterized by FT-IR, XRD, TGA and SEM. FT-IR demonstrated that the reaction products bound covalently to the free amino groups or hydroxyl groups of chitosan via band of amide I or ester band. XRD showed a reduced packing density for grafted chitosan comparing to original chitosan. TGA demonstrated that CTS-g-CADs have a higher thermostability than chitosan. Additionally, chitosan and its derivatives showed similar antibacterial activity. However, the IC50 value of the chitosan-caffeic acid derivative (CTS-g -CA) against the mulberry bacterial wilt pathogen RS-5 was 0.23 mg/mL, which was two-fifths of the IC50 value of chitosan. Therefore, the enzymatically synthesized chitosan polymers can be used to control plant diseases in biotechnological domains. (C) 2016 Elsevier B.V. All rights reserved.

分类号: Q71

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