Quantitative determination of major capsaicinoids in serum by ELISA and time-resolved fluorescent immunoassay based on monoclonal antibodies

文献类型： 外文期刊

第一作者： Yang, Qingqing

作者： Yang, Qingqing;Zhu, Jianguo;Ma, Fei;Li, Peiwu;Zhang, Liangxiao;Zhang, Wen;Ding, Xiaoxia;Zhang, Qi;Yang, Qingqing;Zhu, Jianguo;Ma, Fei;Li, Peiwu;Zhang, Wen;Ding, Xiaoxia;Yang, Qingqing;Zhu, Jianguo;Ma, Fei;Li, Peiwu;Zhang, Qi;Yang, Qingqing;Zhu, Jianguo;Li, Peiwu;Zhang, Liangxiao;Ding, Xiaoxia;Zhang, Qi;Ma, Fei;Li, Peiwu;Zhang, Liangxiao;Zhang, Wen;Ding, Xiaoxia

作者机构：

关键词： Capsaicin;Capsaicinoids;Monoclonal antibody;Indirect competitive enzyme-linked immunosorbent assay (icELISA);Time-resolved fluorescent immunochromatographic assay (TRFICA)

期刊名称：BIOSENSORS & BIOELECTRONICS （ 影响因子：10.618； 五年影响因子：9.323 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： To monitor capsaicinoids in serum on-site, three new monoclonal antibodies (mAbs) were firstly proposed using a conjugate of 4-[(4-hydroxy-3-methoxybenzyl) amino]-4-oxobutanoic acid as the immunogen. Among them, the YQQD8 mAb showed the highest sensitivity and cross-reactivity to major capsaicinoids, such as capsaicin, dihydrocapsaicin and N-vanillylnonanamide. A competitive indirect enzyme-linked immunosorbent assay (icELISA) and a time-resolved fluorescent immunochromatographic assay (TRFICA) were established based on this mAb. The linear range was 1.1-27.0 ng mL(-1) for icELISA and 1.9-62.5 ng mL(-1) for TRFICA and the limit of detection (LOD) of TRFICA was 1.5 ng mL(-1). To decrease the interference of sample components and increase accuracy, serum samples were diluted four times before assays. As a result, the linear range of serum samples was 4.6-107.9 ng mL(-1) for icELISA and 7.6-250.0 ng mL(-1) for TRFICA. Both icELISA and TRFICA showed good recoveries (91.0-112.8% for icELISA and 87.6-111.5% for TRFICA) and concordant results in spiked experiments. Overall, this is the first report of immunoassay based on the mAbs for quantitative determination of major capsaicinoids, and the results demonstrate that both methods can meet the demands of rapid on-site assay for capsaicinoids in serum samples. (C) 2016 Elsevier B.V. All rights reserved.

分类号： Q

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