Lipidomic profiling of tryptophan hydroxylase 2 knockout mice reveals novel lipid biomarkers associated with serotonin deficiency

文献类型： 外文期刊

第一作者： Weng, Rui

作者： Weng, Rui;Shen, Sensen;Yang, Li;Bai, Yu;Liu, Huwei;Weng, Rui;Burton, Casey;Burton, Casey;Nie, Honggang;Tian, Yonglu

作者机构：

关键词： Serotonin deficiency;Tryptophan hydroxylase 2 knockout (Tph2-/-) mice;Lipidomics;Lipid biomarkers;Two-dimensional liquid chromatography-quadrupole time-of-flight mass spectrometry (2D LC-QToF-MS)

期刊名称：ANALYTICAL AND BIOANALYTICAL CHEMISTRY （ 影响因子：4.142； 五年影响因子：3.863 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Serotonin is an important neurotransmitter that regulates a wide range of physiological, neuropsychological, and behavioral processes. Consequently, serotonin deficiency is involved in a wide variety of neurodegenerative diseases, including Alzheimer's disease, Parkinson's disease, schizophrenia, and depression. The pathophysiological mechanisms underlying serotonin deficiency, particularly from a lipidomics perspective, remain poorly understood. This study therefore aimed to identify novel lipid biomarkers associated with serotonin deficiency by lipidomic profiling of tryptophan hydroxylase 2 knockout (Tph2-/-) mice. Using a high-throughput normal-/reversed-phase two-dimensional liquid chromatography-quadrupole time-of-flight mass spectrometry (NP/RP 2D LC-QToF-MS) method, 59 lipid biomarkers encompassing glycerophospholipids (glycerophosphocholines, lysoglycerophosphocholines, glycerophosphoethanolamines, lysoglycerophosphoethanolamines glycerophosphoinositols, and lysoglycerophosphoinositols), sphingolipids (sphingomyelins, ceramides, galactosylceramides, glucosylceramides, and lactosylceramides) and free fatty acids were identified. Systemic oxidative stress in the Tph2-/- mice was significantly elevated, and a corresponding mechanism that relates the lipidomic findings has been proposed. In summary, this work provides preliminary findings that lipid metabolism is implicated in serotonin deficiency.

分类号： O65

  • 相关文献

[1]Lipidomics profiling of goat milk, soymilk and bovine milk by UPLC-Q-Exactive Orbitrap Mass Spectrometry. Li, Qiangqiang,Zhao, Yan,Zhu, Dan,Pang, Xiumei,Liu, Yue,Chen, Gang,Li, Qiangqiang,Frew, Russell.

[2]Identification of diagnostic biomarkers and metabolic pathway shifts of heat-stressed lactating dairy cows. Tian, He,Zheng, Nan,Li, Songli,Zhang, Yangdong,Wang, Jiaqi,Wang, Weiyu,Cheng, Jianbo.

[3]Lipidomics analysis unravels the effect of nitrogen fertilization on lipid metabolism in tea plant (Camellia sinensis L.). Liu, Mei-Ya,Ma, Lifeng,Zhang, Qunfeng,Tang, Dandan,Ruan, Jianyun,Burgos, Asdrubal,Tang, Dandan. 2017

[4]UPLC-Q-Exactive Orbitrap/MS-Based Lipidomics Approach To Characterize Lipid Extracts from Bee Pollen and Their in Vitro Anti-Inflammatory Properties. Li, Qiangqiang,Liang, Xinwen,Xue, Xiaofeng,Wang, Kai,We, Liming,Li, Qiangqiang,Liang, Xinwen,Xue, Xiaofeng,Wang, Kai,We, Liming,Li, Qiangqiang,Liang, Xinwen,Xue, Xiaofeng,Wang, Kai,Zhao, Liang,Zhang, Zhongyin.

[5]A simultaneous extraction method for metabolome and lipidome and its application in cry1Ac and sck-transgenic rice leaf treated with insecticide based on LC-MS analysis. Chang, Yuwei,Lu, Xin,Zhao, Chunxia,Zhang, Junjie,Chen, Shili,Zhao, Yanni,Xu, Guowang,Zhang, Lei,Zhu, Zhen,Wang, Feng.

作者其他论文 更多>>

微信小程序