Protective efficacy of pVAX-RON5p against acute and chronic infections of Toxoplasma gondii in BALB/c mice

文献类型: 外文期刊

第一作者: Zhao, Yu

作者: Zhao, Yu;Li, Zhong-Yuan;Chen, Jia;Liu, Shan-Shan;Zhu, Xing-Quan;Zhou, Dong-Hui;Zhao, Yu;Sun, Xiao-Lin;Li, Zhong-Yuan;Liu, Shan-Shan;Zhu, Xing-Quan

作者机构:

关键词: Toxoplasma gondii;RON5p;DNA vaccine;Protective efficacy

期刊名称:EXPERIMENTAL PARASITOLOGY ( 影响因子:2.011; 五年影响因子:2.132 )

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收录情况: SCI

摘要: Toxoplasma gondii can infect all the warm-blooded animals and humans and causes serious diseases especially in immuno-compromised patients and pregnant women. Rhoptry neck proteins (RONs) play an important role in the formation of moving junction, which mediates the invasion of this parasite. A recombinant plasmid pVAX-RON5p, which can express part of RON5 protein in the eukaryocyte, was generated and used to immune BALB/c mice for evaluating the protective efficacy against the acute and chronic infections of T. gondii. Both humoral and cellular immune responses were evoked in mice by pVAX-RON5p immunization, and a slightly prolonged survival period was detected in the immunized group (7.6 +/- 3.31 days) compared to the blank control (4.9 +/- 0.32 days) after acute T. gondii infection (P < 0.05). For chronic infection of T. gondii, the number of cysts in the brain of pVAX-RON5p-immunized mice decreased 25.8% compared to blank control (P < 0.05). Our data suggested that RON5p DNA vaccine can induce partial protective immunity against acute and chronic T. gondii infections. (C) 2016 Elsevier Inc. All rights reserved.

分类号: Q958.9

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