Application of marker-assisted backcross to introgress Bph3, Bph14 and Bph15 into an elite indica rice variety for improving its resistance to brown planthopper

文献类型： 外文期刊

第一作者： Hu, Wei

作者： Hu, Wei;Jiang, Yijun;Xiao, Hanxiang;Zhang, Yang;Hu, Kan

作者机构：

关键词： Oryza sativa L;brown planthopper-resistance gene;marker-assisted selection;near-isogenic lines;pyramided lines;agronomic trait

期刊名称：PLANT BREEDING （ 影响因子：1.832； 五年影响因子：1.956 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： To improve brown planthopper (Nilaparvata lugens Stal; BPH) resistance of an elite indica cultivar of South China, Hemeizhan (HMZ), we applied marker-assisted backcross (MABC) to incorporate three BPH-resistance genes (Bph3, Bph14 and Bph15) into the genetic background of HMZ. In the third backcross (BC3) generation, we obtained near-isogenic lines (Bph3-NIL, Bph14-NIL, Bph15-NIL and Bph14+Bph15-NIL) with more than 96% recovery of recurrent parent genome, and pyramided lines (Bph3+Bph14-PYL, Bph3+Bph15-PYL and Bph3+Bph14+Bph15-PYL) with more than 89% recovery of recurrent parent genome. These lines showed stronger resistance against BPH than HMZ at seedling and booting stages. The rank of resistance gene effect was Bph3+Bph14+Bph15Bph3+Bph15Bph3+Bph14Bph14+Bph15Bph3Bph15Bph14>none. Compared with HMZ, only Bph3+Bph14+Bph15-PYL had a significant difference in yield per plant, and the lines carrying Bph3 had higher amylose contents, indicating that Bph3 was tightly linked to Wx(a) allele. These improved lines are good intermediate sources of broad-spectrum and durable BPH resistance to improve other indica cultivars. Our results demonstrate that MABC is a very efficient approach to improve BPH resistance of elite rice cultivar.

分类号： S3

  • 相关文献

[1]Identification of SSR markers for a broad-spectrum blast resistance gene Pi20(t) for marker-assisted breeding. Wan, Jianmin,Li, Wei,Jia, Yulin. 2008

[2]Identification of the genes and pathways associated with pigment gland morphogenesis in cotton by transcriptome profiling of near-isogenic lines. Quan Sun,Shengwei Li,Min Chen,Yingfan Cai,Jianchuan Mo,Xiaohong He,Huaizhong Jiang,JinggaoLiu,Kairong Lei.

[3]Characterization and identification of cold tolerant near-isogenic lines in rice. Zhou, Lei,Hu, Guanglong,Pan, Yinghua,Zhang, Hongliang,Li, Jinjie,Li, Zichao,Zhou, Lei,Hu, Guanglong,Pan, Yinghua,Zhang, Hongliang,Li, Jinjie,Li, Zichao,Zeng, Yawen,Yang, Shuming,Zhou, Lei,You, Aiqing,Hu, Guanglong. 2012

[4]Effect of wx genes on amylose content, physicochemical properties of wheat starch, and the suitability of waxy genotype for producing Chinese crisp sticks. Ma, Hongbo,Lv, Guofeng,Wang, Xiue,Cheng, Shunhe,Zhang, Xiao,Gao, Derong,Zhang, Boqiao,Lv, Guofeng,Wu, Ronglin,Cheng, Xiaoming,Cheng, Shunhe,Bie, Tongde,Wang, Canguo. 2013

[5]Determination of Mineral Elements in Brown Rice of Near-Isogenic Lines Population for Japonica Rice by ICP-AES. Wang Lu-xiang,Li Qi-wan,Zeng Ya-wen,Sun Zheng-hai,Yang Shu-ming,Du Juan,Pu Xiao-ying,Du Wei,Zeng Ya-wen,Xiao Feng-hui,Sun Zheng-hai. 2008

[6]Lack of fitness costs and inheritance of resistance to Bacillus thuringiensis Cry1Ac toxin in a near-isogenic strain of Plutella xylostella (Lepidoptera: Plutellidae). Zhu, Xun,Yang, Yanjv,Wu, Qingjun,Wang, Shaoli,Xie, Wen,Guo, Zhaojiang,Kang, Shi,Xia, Jixing,Zhang, Youjun.

[7]Effects of the dominant glandless gene Gl(2)(e) on agronomic and fibre characters of Upland cotton. Y.L Yuan,,Y.H Chen,C.M Tang,S.R Jing,S.L Liu,J.J Pan,R.J Kohel,T.Z Zhang. 2000

[8]Precise mapping of a quantitative trait locus interval for spike length and grain weight in bread wheat (Triticum aestivum L.). Wu, Xinyi,Cheng, Ruiru,Xue, Shulin,Kong, Zhongxin,Wan, Hongshen,Li, Guoqiang,Huang, Yulong,Jia, Haiyan,Zhang, Lixia,Ma, Zhengqiang,Jia, Jizeng. 2014

[9]Construction and characterisation of near-isogenic Plutella xylostella (Lepidoptera: Plutellidae) strains resistant to Cry1Ac toxin. Zhu, Xun,Lei, Yanyuan,Yang, Yanjv,Wu, Qingjun,Wang, Shaoli,Xie, Wen,Guo, Zhaojiang,Fu, Wei,Zhang, Youjun,Zhu, Xun,Li, Jianhong,Baxter, Simon W..

[10]Heading date gene, dth3 controlled late flowering in O. Glaberrima Steud. by down-regulating Ehd1. Bian, X. F.,Liu, X.,Zhao, Z. G.,Jiang, L.,Gao, H.,Zhang, Y. H.,Zheng, M.,Chen, L. M.,Liu, S. J.,Wan, J. M.,Zhai, H. Q.,Wan, J. M.. 2011

[11]Development of Near-sogenic Lines in a Parthenogenetically Reproduced Thrips Species, Frankliniella occidentalis. Yuan, Guangdi,Wan, Yanran,Li, Xiaoyu,He, Bingqing,Zhang, Youjun,Xu, Baoyun,Wang, Shaoli,Xie, Wen,Wu, Qingjun,Zhou, Xuguo. 2017

[12]Effects of plant height on type I and type II resistance to fusarium head blight in wheat. Yan, W.,Li, H. B.,Liu, C. J.,Yan, W.,Cai, S. B.,Ma, H. X.,Rebetzke, G. J.,Liu, C. J..

[13]Mutation of rice (Oryza sativa L.) LOX-1/2 near-isogenic lines with ion beam implantation and study of their storability. Song, Mei,Wu, Yuejin,Liu, B. M.,Jiang, J. Y.,Xu, X.,Yu, Z. L.,Zhang, Ying.

[14]Gene Discovery in Triticum dicoccoides, the Direct Progenitor of Cultivated Wheats. Sun, D. F.,Peng, Y. L.,Nevo, E.,Peng, J. H.. 2013

[15]Detection of QTLs for phosphorus use efficiency in relation to agronomic performance of wheat grown under phosphorus sufficient and limited conditions. Su, Jun-Ying,Zheng, Qi,Li, Hong-Wei,Li, Bin,Tong, Yi-Ping,Li, Zhen-Sheng,Jing, Rui-Lian. 2009

[16]Genetic diversity and marker-trait association analysis for agronomic traits in Aconitum carmichaelii Debeaux. Yu, Ma,Cao, Ling-Li,Shu, Xiao-Yan,Huang, Jing,Liu, Dan,Zhang, Hong,Hou, Da-Bin,Yang, Yu-Xia,Guan, Ling-Liang,Gou, Lu-Lu. 2017

[17]Identification of Quantitative Trait Loci for Bacterial Blight Resistance Derived from Oryza meyeriana and Agronomic Traits in Recombinant Inbred Lines of Oryza sativa. Chen, Li-Na,Yang, Yong,Yan, Cheng-Qi,Wang, Ming,Yu, Chu-Lang,Zhou, Jie,Cheng, Ye,Cheng, Xiao-Yue,Chen, Jian-Ping,Chen, Li-Na,Zhang, Wei-Lin,Cheng, Xiao-Yue. 2012

[18]Development of Isogenic Lines of Morphological Markers in Indica Rice. Zeng, DL,Qian, Q,Dong, GJ,Zhu, XD,Dong, FG,Teng, S,Luo, LB,Cao, LY,Cheng, SH,Xiong, ZM.

[19]Discovery and transmission of functional QTL in the pedigree of an elite soybean cultivar Suinong14. Yang, R. Q.,Qin, J.,Li, Y. H.,Guan, R. X.,Chang, R. Z.,Qiu, L. J.,Qin, J.,Jiang, C. X.,Li, W. B..

[20]Fine Mapping of qHD8-1, a QTL Controlling the Heading Date, to a 26-kb DNA Fragment in Rice (Oryza sativa L.). Liu, Xu,Wang, Wenying,Ding, Hanfeng,Yao, Fangyin,Pei, Chengguo,Zhu, Changxiang,Wen, Fujiang,Jiang, Mingsong,Li, Guangxian.

作者其他论文 更多>>

微信小程序