Linoleic acid and stearic acid elicit opposite effects on AgRP expression and secretion via TLR4-dependent signaling pathways in immortalized hypothalamic N38 cells

文献类型： 外文期刊

第一作者： Wang, Songbo

作者： Wang, Songbo;Xiang, Nana;Yang, Liusong;Zhu, Canjun;Zhu, Xiaotong;Wang, Lina;Gao, Ping;Xi, Qianyun;Zhang, Yongliang;Shu, Gang;Jiang, Qingyan;Wang, Songbo;Xiang, Nana;Yang, Liusong;Zhu, Canjun;Zhu, Xiaotong;Wang, Lina;Gao, Ping;Xi, Qianyun;Zhang, Yongliang;Shu, Gang;Jiang, Qingyan;Wang, Songbo;Xiang, Nana;Yang, Liusong;Zhu, Canjun;Zhu, Xiaotong;Wang, Lina;Gao, Ping;Xi, Qianyun;Zhang, Yongliang;Shu, Gang;Jiang, Qingyan;Wang, Songbo;Xiang, Nana;Yang, Liusong;Zhu, Canjun;Zhu, Xiaotong;Wang, Lina;Gao, Ping;Xi, Qianyun;Zhang, Yongliang;Shu, Gang;Jiang, Qingyan

作者机构：

关键词： Linolenic acid;Stearic acid;N38 cells;TLR4;AgRP;Obesity

期刊名称：BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS （ 影响因子：3.575； 五年影响因子：3.381 ）

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收录情况： SCI

摘要： The regulation of food intake is a promising way to combat obesity. It has been implicated that various fatty acids exert different effects on food intake and body weight. However, the underlying mechanism remains poorly understood. The aim of the present study was to investigate the effects of linoleic acid (LA) and stearic acid (SA) on agouti-related protein (AgRP) expression and secretion in immortalized mouse hypothalamic N38 cells and to explore the likely underlying mechanisms. Our results demonstrated that LA inhibited, while SA stimulated AgRP expression and secretion of N38 cells in a dose dependent manner. In addition, LA suppressed the protein expression of toll-like receptor 4 (TLR4), phosphorylation levels of JNK and IKK alpha/beta, suggesting the inhibition of TLR4-dependent inflammation pathway. However, the above mentioned inhibitory effects of LA were eliminated by TLR4 agonist lipopolysaccharide (LPS). In contrast, SA promoted TLR4 protein expression and activated TLR4-dependent inflammation pathway, with elevated ratio of p-JNK/JNK. While TLR4 siRNA reversed the stimulatory effects of SA on AgRP expression and TLR4-dependent inflammation. Moreover, we found that TLR4 was also involved in LA-enhanced and SA-impaired leptin/insulin signal pathways in N38 cells. In conclusion, our findings indicated that LA elicited inhibitory while SA exerted stimulatory effects on AgRP expression and secretion via TLR4-dependent inflammation and leptin/insulin pathways in N38 cells. These data provided a better understanding of the mechanism underlying fatty acids-regulated food intake and suggested the potential role of long-chain unsaturated fatty acids such as LA in reducing food intake and treating obesity. (C) 2016 Elsevier Inc. All rights reserved.

分类号： Q5

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