bFGF signaling-mediated reprogramming of porcine primordial germ cells

文献类型： 外文期刊

第一作者： Zhang, Yu

作者： Zhang, Yu;Ma, Jing;Lv, Jiawei;Wei, Renyue;Cong, Yimei;Liu, Zhonghua;Li, Hai;Cong, Yimei

作者机构：

关键词： Primordial germ cells;Embryonic germcells;Differentiation;bFGF;Bama miniature pig

期刊名称：CELL AND TISSUE RESEARCH （ 影响因子：5.249； 五年影响因子：4.588 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Primordial germ cells (PGCs) have the ability to be reprogrammed into embryonic germ cells (EGCs) in vitro and are an alternative source of embryonic stem cells. Other than for the mouse, the systematic characterization of mammalian PGCs is still lacking, especially the process by which PGCs convert to pluripotency. This hampers the understanding of germ cell development and the derivation of authenticated EGCs from other species. We observed the morphological development of the genital ridge from Bama miniature pigs and found primary sexual differentiation in the E28 porcine embryo, coinciding with Blimp1 nuclear exclusion in PGCs. To explore molecular events involved in porcine PGC reprogramming, transcriptome data of porcine EGCs and fetal fibroblasts (FFs) were assembled and 1169 differentially expressed genes were used for Gene Ontology analysis. These genes were significantly enriched in cell-surface receptor-linked signal transduction, in agreement with the activation of LIF/Stat3 signaling and FGF signaling during the derivation of porcine EG-like cells. Using a growth-factor-defined culture system, we explored the effects of bFGF on the process and found that bFGF not only functioned at the very beginning of PGC dedifferentiation by impeding Blimp1 nuclear expression via a PI3K/AKT-dependent pathway but also maintained the viability of cultured PGCs thereafter. These results provide further insights into the development of germ cells from livestock and the mechanism of porcine PGC reprogramming.

分类号： Q2

  • 相关文献

[1]Embryoid bodies formation from chicken primordial germ cells. Xiong, Hui,Pu, Yabin,Shan, Zhiqiang,Hu, Pengfei,Guan, Weijun,Ma, Yuehui,Xiong, Hui,Hu, Qingyun.

[2]CLONING OF BASIC FIBROBLAST GROWTH FACTOR FROM CHINESE SMALL TAIL HAN SHEEP AND ITS EFFECTS ON PROLIFERATION OF MURINE C2C12 MYOBLASTS. Liu, Hua-zhong,Chen, Shao-hong,Luo, Ping,Shang, Jiang-hua. 2013

[3]Genetic variability assessed by microsatellites in the breeding populations of the shrimp Penaeus (Fenneropenaeus) chinensis in China. Zhang, Tianshi,Kong, Jie,Wang, Weiji,Wang, Qingyin,Zhang, Tianshi.

[4]Establishment of a pluripotent embryonic cell line from sea perch (Lateolabrax japonicus) embryos. Cher, SL,Sha, ZX,Ye, HQ.

[5]Chicken model for inducing primordial germ cells differentiating into motile sperms in vitro. Xiong, Hui,Li, Xiangchen,Hu, Pengfei,Guan, Weijun,Hu, Qingyun. 2017

[6]Cultivation and Biological Characterization of Chicken Primordial Germ Cells. Ji, Meng,Guan, Wei-jun,Gao, Yu-hua,Bai, Chun-yu,Ma, Yue-hui,Li, Xiang-chen,Li, Lu. 2016

[7]Generation of chimeric piglets by injection of embryonic germ cells from inbred Wuzhishan miniature pigs into blastocysts. Dong, Xiao,Mu, Yulian,Liu, Lixin,Chen, Hongping,Zhang, Li,Feng, Shutang,Dong, Xiao,Tsung, Hsiaochien,Mu, Yulian,Feng, Shutang,Wang, Hongjun. 2014

[8]Recent progress on technologies and applications of transgenic poultry. Li, Jin-Jun,Lu, Li-Zhi. 2010

[9]Molecular characterization, sexually dimorphic expression, and functional analysis of 3'-untranslated region of vasa gene in half-smooth tongue sole (Cynoglossus semilaevis). Huang, Jinqiang,Huang, Jinqiang,Lin, Fan.

[10]Identification, characterization and functional analysis of regulatory region of nanos gene from half-smooth tongue sole (Cynoglossus semilaevis). Huang, Jinqiang,Shao, Changwei,Wang, Na,Chen, Songlin,Huang, Jinqiang,Shao, Changwei,Wang, Na,Chen, Songlin,Huang, Jinqiang,Li, Yongjuan.

[11]MicroRNA-95 promotes myogenic differentiation by down-regulation of aminoacyl-tRNA synthase complex-interacting multifunctional protein 2. Li, Biao,Xie, Shanshan,Cai, Chunbo,Qian, Lili,Jiang, Shengwang,Ma, Dezun,Xiao, Gaojun,Gao, Ting,Cui, Wentao,Yang, Jinzeng. 2017

[12]Genetic diversity and differentiation of Camellia sinensis L. (cultivated tea) and its wild relatives in Yunnan province of China, revealed by morphology, biochemistry and allozyme studies. Jin, C,Wang, PS,Xia, YM,Xu, M,Pei, SJ. 2005

[13]Isolation and Characterization of Mesenchymal Stem Cells Derived from Duck Embryo Liver. Wang, Wenjie,Guan, Weijun,Ma, Yuehui,Huang, Hongmei,Li, Qian,Wang, KunFu,Chen, Jia. 2017

[14]Klf7 modulates the differentiation and proliferation of chicken preadipocyte. Zhang, Zhiwei,Wang, Haixia,Sun, Yingning,Li, Hui,Wang, Ning,Zhang, Zhiwei,Wang, Haixia,Sun, Yingning,Li, Hui,Wang, Ning,Zhang, Zhiwei,Wang, Haixia,Sun, Yingning,Li, Hui,Wang, Ning,Sun, Yingning. 2013

[15]Direct and indirect shoot and bulblet regeneration from cultured leaf explants of Lilium pumilum, an endangered species. Jin, Shumei,Wang, Ji,Sun, Dan,Liu, Shengkui,Wang, Xinwang,Genovesi, A. D.,Li, Guoliang. 2014

[16]Cocktail supplement with rosiglitazone: a novel inducer for chicken preadipocyte differentiation in vitro. Cheng, Bohan,Wu, Mengqi,Xu, Songsong,Zhang, Xinyang,Wang, Yuxiang,Wang, Ning,Leng, Li,Li, Hui,Cheng, Bohan,Wu, Mengqi,Xu, Songsong,Zhang, Xinyang,Wang, Yuxiang,Wang, Ning,Leng, Li,Li, Hui,Cheng, Bohan,Wu, Mengqi,Xu, Songsong,Zhang, Xinyang,Wang, Yuxiang,Wang, Ning,Leng, Li,Li, Hui. 2016

[17]IGF1 regulates RUNX1 expression via IRS1/2: Implications for antler chondrocyte differentiation. Yang, Zhan-Qing,Zhang, Hong-Liang,Geng, Shuang,Wang, Kai,Yu, Hai-Fan,Yue, Zhan-Peng,Guo, Bin,Duan, Cui-Cui. 2017

[18]Oleate promotes differentiation of chicken primary preadipocytes in vitro. Shang, Zhouchun,Guo, Lin,Wang, Ning,Shi, Hui,Wang, Yuxiang,Li, Hui,Shang, Zhouchun,Guo, Lin,Wang, Ning,Shi, Hui,Wang, Yuxiang,Li, Hui,Shang, Zhouchun,Guo, Lin,Wang, Ning,Shi, Hui,Wang, Yuxiang,Li, Hui. 2014

[19]Immortalized bovine mammary epithelial cells express stem cell markers and differentiate in vitro. Hu, Han,Zheng, Nan,Hu, Han,Gao, Haina,Dai, Wenting,Zhang, Yangdong,Li, Songli,Wang, Jiaqi.

[20]Seed coat development and its evolutionary implications in diploid and amphidiploid Brassica species. Zeng, Chang-Li,Wu, Xiao-Ming,Wang, Jian-Bo.

作者其他论文 更多>>

微信小程序