Molecular cloning and expression analysis on LPL of Coilia nasus

文献类型： 外文期刊

第一作者： Wang, Meiyao

作者： Wang, Meiyao;Xu, Dongpo;Liu, Kai;Yang, Jian;Xu, Pao;Wang, Meiyao;Xu, Dongpo;Liu, Kai;Yang, Jian;Xu, Pao

作者机构：

关键词： Coilia nasus;Lipoprotein lipase;Homology;Expression

期刊名称：GENE （ 影响因子：3.688； 五年影响因子：3.329 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Coilia nasus is one important commercial anadromous species which mainly distributed in the Yangtze River in China. At present, it has been on the "National Key Protective Species List" because of its severe resource damage. Lipid metabolism is very important during its long-distance migration. To make further research on lipid metabolism of C. nasus, we cloned lipoprotein lipase gene with homologous cloning method. A full-length cDNA of LPL of C nasus was cloned from liver which covered 3537 bp with a 1519 bp open reading frame encoding 505 deduced amino acids whose molecular mass was 57.5 kDa and theoretical isoelectric point was 7.58. The deduced amino acids had high similarity with the reported LPL sequence of other species. It had typical conserved domain of LPL protein containing catalytic triad, N-linked glycosylation sites and conserved heparin-binding site, etc. We adopted quantitative real-time RT-PCR method to detect the mRNA expression of LPL of C nasus in ten tissues including mesenteric adipose, liver, muscle, stomach, spleen, heart, head kidney, trunk kidney, gill and brain with beta-actin as internal reference. LPL expressed in all the detected tissues. The highest expression was in mesenteric adipose, and followed by liver, muscle, stomach. Lipid expressed lowly in spleen, heart, head kidney, trunk kidney, gill and brain. The research on the cloning and differential expression of LPL of C. nasus will lay foundation for further research on lipid metabolism of C. nasus. (C) 2016 Elsevier B.V. All rights reserved.

分类号： R394

  • 相关文献

[1]Changes in the activities and mRNA expression levels of lipoprotein lipase (LPL), hormone-sensitive lipase (HSL) and fatty acid synthetase (FAS) of Nile tilapia (Oreochromis niloticus) during fasting and re-feeding. Tian, Juan,Wen, Hua,Zeng, Ling-Bing,Jiang, Ming,Wu, Fan,Liu, Wei,Yang, Chang-Geng. 2013

[2]Effects of high ambient temperature on lipid metabolism in finishing pigs. Wu Xin,Li Ze-yang,Jia An-feng,Su Hong-guang,Hu Chun-hong,Zhang Min-hong,Feng Jing-hai,Li Ze-yang.

[3]Effects of chromium picolinate on fat deposition, activity and genetic expression of lipid metabolism-related enzymes in 21 day old Ross broilers. Chen, Guangxin,Chu, Wenhui,Li, Chunyi,Zhao, Haiping,Chen, Guangxin,Chu, Wenhui,Li, Chunyi,Zhao, Haiping,Chen, Guangxin,Gao, Zhenhua,Cao, Zan. 2018

[4]Seasonal and nutrients intake regulation of lipoprotein lipase (LPL) activity in grazing yak (Bos grunniens) in the Alpine Regions around Qinghai Lake. Yan, P.. 2012

[5]Developmental Expression of HSP60 and HSP10 in the Coilia nasus Testis during Upstream Spawning Migration. Fang, Di-An,Zhou, Yan-Feng,Zhang, Min-Ying,Xu, Dong-Po,Liu, Kai,Duan, Jin-Rong,Fang, Di-An,Xu, Dong-Po. 2017

[6]Molecular Characteristic, Protein Distribution and Potential Regulation of HSP90AA1 in the Anadromous Fish Coilia nasus. Fang, Di-An,Duan, Jin-Rong,Zhou, Yan-Feng,Zhang, Min-Ying,Xu, Dong-Po,Liu, Kai,Xu, Pao,Fang, Di-An,Xu, Dong-Po. 2016

[7]First studies of embryonic and larval development of Coilia nasus (Engraulidae) under controlled conditions. Xu, Gangchun,Tang, Xue,Gu, Ruobo,Xu, Pao,Tang, Xue,Le, Guowei,Zhang, Chengxiang,Zheng, Jinliang. 2011

[8]Molecular characterization and differential expression of the myostatin gene in Coilia nasus. Du, Fukuan,Xu, Gangchun,Nie, Zhijuan,Xu, Pao,Gu, Ruobo.

[9]Screening potential SSR markers of the anadromous fish Coilia nasus by de novo transcriptome analysis using Illumina sequencing. Fang, D. -A.,Xu, P.,Fang, D. -A.,Zhou, Y. -F.,Duan, J. -R.,Zhang, M. -Y.,Xu, D. -P.,Liu, K.,Xu, P.,Wei, Q.. 2015

[10]Differences in muscle cellularity and flesh quality between wild and farmed Coilia nasus (Engraulidae). Tang, Xue,Xu, Gangchun,Xu, Pao,Gu, Ruobo,Tang, Xue,Dai, Hui,Zhang, Chengxiang. 2012

[11]Transcriptome analysis gene expression in the liver of Coilia nasus during the stress response. Du, Fukuan,Xu, Gangchun,Nie, Zhijuan,Xu, Pao,Gu, Ruobo. 2014

[12]Molecular cloning, tissue and embryonic development expression, and appetite regulatory effect of pancreatic peptide Y in Coilia nasus. Yang, Siyu,Xu, Gangchun,Xu, Pao,Xu, Gangchun,Du, Fukuan,Xu, Pao.

[13]Testes transcriptome profiles of the anadromous fish Coilia nasus during the onset of spermatogenesis. Zhou, Yan-Feng,Duan, Jin-Rong,Liu, Kai,Xu, Dong-Po,Zhang, Min-Ying,Fang, Di-An,Xu, Pao,Fang, Di-An,Xu, Pao.

[14]Ovary transcriptome profiling of Coilia nasus during spawning migration stages by Illumina sequencing. Shi, Ying,Wei, Qi-Wei,Duan, Jin-Rong,Zhou, Yan-Feng,Xu, Dong-Po,Zhang, Min-Ying,Liu, Kai,Fang, Di-An,Fang, Di-An.

[15]The cloning and sequencing of a cDNA encoding a WD repeat protein in cotton (Gossypium hirsutum L.). HONG Y. DUAN,FU G. LI,XU D. WU,DONG M. MA,MAO WANG,YU X. HOU.

[16]An Overlooked Paleotetraploidization in Cucurbitaceae. Wang, Jinpeng,Li, Yuxian,Liu, Yinzhe,Yang, Nanshan,Yu, Jigao,Ma, Xuelian,Sun, Sangrong,Xia, Ruiyan,Liu, Xiaojian,Ge, Dongcen,Luo, Sainan,Liu, Yinmeng,Kong, Youting,Cui, Xiaobo,Lei, Tianyu,Wang, Li,Wang, Zhenyi,Ge, Weina,Zhang, Lan,Song, Xiaoming,Yuan, Min,Guo, Di,Pan, Yuxin,Yang, Guixian,Xiao, Yue,Sun, Jinshuai,Zhang, Cong,Li, Zhibo,Xu, Haiqing,Duan, Xueqian,Shen, Shaoqi,Wang, Xiyin,Wang, Jinpeng,Sun, Pengchuan,Li, Yuxian,Liu, Yinzhe,Yang, Nanshan,Yu, Jigao,Sun, Sangrong,Lei, Tianyu,Wang, Li,Wang, Zhenyi,Ge, Weina,Zhang, Lan,Song, Xiaoming,Yuan, Min,Guo, Di,Jin, Dianchuan,Chen, Wei,Pan, Yuxin,Liu, Tao,Wang, Xiyin,Zhang, Zhonghua,Huang, Sanwen. 2018

[17]Structural Homologies of Major Immune-related Genes between Duck and Chicken: Implications on Differential Avian Resistance Against Influenza Virus. Li, G.,Lu, L.. 2011

[18]Molecular Characterization of Southern Rice Blacked-Streaked Dwarf Virus (SRBSDV) from Vietnam. Xue, Jin,Li, Jing,Zhang, Heng-Mu,Yang, Jian,Lv, Ming-Fang,Chen, Jian-Ping,Xue, Jin,Gao, Bi-Da,Lv, Ming-Fang. 2014

[19]Porcine skeletal muscle differentially expressed gene CMYA1: isolation, characterization, mapping, expression and association analysis with carcass traits. Xu, X. L.,Xu, X. W.,Pan, P. W.,Yu, M.,Liu, B.,Xu, X. L.,Xu, X. W.,Pan, P. W.,Yu, M.,Liu, B.,Li, K.,Jiang, Z. H.,Rothschild, M. F.,Rothschild, M. F..

[20]Sequence analysis shows that a dwarfing disease on rice, wheat and maize in China is caused by rice black-streaked dwarf virus. Zhang, HM,Chen, JP,Lei, JL,Adams, MJ. 2001

作者其他论文 更多>>

微信小程序