Simultaneous determination of capsaicin and dihydrocapsaicin for vegetable oil adulteration by immunoaffinity chromatography cleanup coupled with LC-MS/MS
文献类型: 外文期刊
第一作者: Ma, Fei
作者: Ma, Fei;Yang, Qingqing;Li, Peiwu;Zhang, Qi;Zhang, Liangxiao;Ma, Fei;Yang, Qingqing;Li, Peiwu;Zhang, Qi;Zhang, Liangxiao;Ma, Fei;Yang, Qingqing;Li, Peiwu;Zhang, Qi;Zhang, Liangxiao;Ma, Fei;Yang, Qingqing;Li, Peiwu;Zhang, Qi;Ma, Fei;Li, Peiwu;Zhang, Liangxiao;Matthaeus, Bertrand
作者机构:
关键词: Capsaicinoids;Immunoaffinity chromatography;Isotope dilution internal standard;LC-MS/MS;Inedible waste oil
期刊名称:JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES ( 影响因子:3.205; 五年影响因子:3.068 )
ISSN:
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收录情况: SCI
摘要: Capsaicin and dihydrocapsaicin were selected as adulteration markers to authenticate vegetable oils. In this study, a method of immunoaffinity chromatography (IAC) combined with liquid chromatography-tandem mass spectrometry was established for the determination of capsaicin and dihydrocapsaicin in vegetable oils. In this method, immunosorbents were obtained by covalently coupling highly specific capsaicinoid polyclonal antibodieswith CNBr-activated Sepharose 4B, and then packed into a polyethylene column. In this paper, the major parameters affecting IAC extraction efficiency, including loading, washing and eluting conditions, were also investigated. The IAC column displayed high selectivity for capsaicin and dihydrocapsaicin with the maximum capacity of 240 ng. The limit of detection (LOD) and limit of quantification (LOQ) for capsaicin were calculated as 0.02 and 0.08 mu g kg(-1), and for dihydrocapsaicin were 0.03 and 0.10 mu g kg(-1). The recoveries of capsaicin and dihydrocapsaicin in oil samples were in the range of 87.3-95.2% with the relative standard deviation (RSD) of less than 6.1%. The results indicated that capsaicinoid compounds could not be found in edible vegetable oils. Therefore, the proposed method is simple, reliable and adequate for routine monitoring of capsaicinoid compounds in vegetable oils and has an excellent potential for detection of adulteration with inedible waste oil. (C) 2015 Elsevier B.V. All rights reserved.
分类号: Q503
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