Preparative Isolation of Two Prenylated Biflavonoids from the Roots and Rhizomes of Sinopodophyllum emodi by Sephadex LH-20 Column and High-Speed Counter-Current Chromatography

文献类型： 外文期刊

第一作者： Sun, Yan-Jun

作者： Sun, Yan-Jun;Pei, Li-Xin;Wang, Jun-Min;Zhang, Yan-Li;Gao, Mei-Ling;Ji, Bao-Yu;Sun, Yan-Jun;Pei, Li-Xin;Wang, Jun-Min;Zhang, Yan-Li;Gao, Mei-Ling;Ji, Bao-Yu;Wang, Kai-Bo;Wang, Kai-Bo;Sun, Yin-Shi

作者机构：

关键词： Sinopodophyllum emodi;prenylated biflavonoid;Sephadex LH-20 column chromatography;high-speed counter-current chromatography;cytotoxic activity

期刊名称：MOLECULES （ 影响因子：4.411； 五年影响因子：4.587 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Two prenylated biflavonoids, podoverines B- C, were isolated from the dried roots and rhizomes of Sinopodophyllum emodi using a Sephadex LH- 20 column ( SLHC) and high- speed counter- current chromatography ( HSCCC). The 95% ethanol extract was partitioned with ethyl acetate in water. Target compounds from the ethyl acetate fraction were further enriched and purified by the combined application of SLHC and HSCCC. n- Hexane- ethyl acetate- methanol- water ( 3.5: 5: 3.5: 5, v/ v) was chosen as the two phase solvent system. The flow rate of mobile phase was optimized at 2.0 mL center dot min - 1. Finally, under optimized conditions, 13.8 mg of podoverine B and 16.2 mg of podoverine C were obtained from 200 mg of the enriched sample. The purities of podoverines B and C were 98.62% and 99.05%, respectively, as determined by HPLC. For the first time, podoverins B and C were found in the genus Sinopodophyllum. Their structures were determined by spectroscopic methods ( HR- ESI- MS, 1H- NMR, 13C- NMR, HSQC, HMBC). Their absolute configurations were elucidated by comparison of their experimental and calculated ECD spectra. The cytotoxic activities were evaluated against MCF- 7 and HepG2 cell lines. The separation procedures proved to be practical and economical, especially for trace prenylated biflavonoids from traditional Chinese medicine.

分类号： O62

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