Molecular cloning, structure and expressional profiles of two novel single-exon genes (PoCCR6A and PoCCR6B) in the Japanese flounder (Paralichthys olivaceus)

文献类型： 外文期刊

第一作者： Wang, Lei

作者： Wang, Lei;Zhang, Yong-zhen;Xu, Wen-teng;Jia, Xiao-dong;Chen, Song-lin

作者机构：

关键词： Japanese flounder;Chemokine receptor 6 (CCR6);Single-exon gene;Bacterial infection

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： CCR6 is an important binding receptor of CCL20 and beta-defensins, and has multiple functions in the innate and acquired immune responses. In this study, we cloned the PoCCR6A and PoCCR6B genes of the Japanese flounder and studied the gene structure and expression patterns of these two genes in bacterial infection. The full-length PoCCR6A cDNA is 1415 bp and the open reading frame (ORF) is 1113 bp, encoding a 370-amino-acid peptide. The full-length PoCCR6B cDNA is 2193 bp and the ORF is 1029 bp, encoding a 363-amino-acid peptide. The structures of PoCCR6A and PoCCR6B indicate that they are single-exon genes. The predicted proteins encoded by PoCCR6A and PoCCR6B have the typical G-protein coupled receptor (GPCR) family signature of seven transmembrane domains and several conserved structural features. A tissue distribution analysis showed that PoCCR6A is predominately expressed in the intestine, gill, and blood, and PoCCR6B in the gill, spleen, and liver. The expression patterns of the two chemokine receptors were analyzed during bacterial infection. In spleen and kidney, the expression of PoCCR6A was significantly upregulated at 24 h after infection, whereas the expression of PoCCR6B was steady at these time points. While in intestine, both of them were upregulated at 6 h-12 h after infection, and in gill the expression levels of them were upregulated at 24 h. The patterns of expression suggested that PoCCR6A and PoCCR6B play an important role in the immune response of the Japanese flounder, especially in the mucosal tissues. (C) 2016 Elsevier Ltd. All rights reserved.

分类号： S9

  • 相关文献

[1]Identification and expression analysis of goose-type lysozyme in half-smooth tongue sole (Cynoglossus semilaevis). Sha, Zhen-Xia,Wang, Qi-Long,Chen, Song-Lin,Liu, Yang. 2012

[2]Genome-wide characterization of Toll-like receptor gene family in common carp (Cyprinus carpio) and their involvement in host immune response to Aeromonas hydrophila infection. Gong, Yiwen,Feng, Shuaisheng,Li, Shangqi,Zhang, Yan,Zhao, Zixia,Jiang, Yanliang,Gong, Yiwen,Hu, Mou,Jiang, Yanliang,Xu, Peng. 2017

[3]Complement factor I from flatfish half-smooth tongue (Cynoglossus semilaevis) exhibited anti-microbial activities. Xiang, Jinsong,Li, Xihong,Chen, Yadong,Lu, Yang,Yu, Mengjun,Chen, Xuejie,Zhang, Wenting,Zeng, Yan,Sun, Luming,Chen, Song Lin,Sha, Zhenxia,Xiang, Jinsong,Chen, Xuejie,Xiang, Jinsong,Chen, Yadong,Lu, Yang,Yu, Mengjun,Chen, Xuejie,Sha, Zhenxia,Li, Xihong,Chen, Song Lin,Yu, Mengjun,Zhang, Wenting,Zeng, Yan,Sun, Luming.

[4]Cloning, molecular characterization and expression analysis of heat shock cognate 70 (Hsc70) cDNA from turbot (Scophthalmus maximus). Wang, T. T.,Wang, N.,Liao, X. L.,Meng, L.,Liu, Y.,Chen, S. L.,Wang, T. T..

[5]beta-1,3-Glucan recognition protein 3 activates the prophenoloxidase system in response to bacterial infection in Ostrinia furnacalis Guenee. Zhao, Ya,Xu, Qiuwen,Wang, Yingjuan,Wang, Libao,Zhang, Yiqiang,Feng, Congjing,Wang, Zhenying,Wang, Zengxia,Song, Qisheng. 2018

[6]Identification of differentially-expressed genes of rice in overlapping responses to bacterial infection by Xanthomonas oryzae pv. oryzae and nitrogen deficiency. Chen Hua-min,Tian Fang,He Chen-Yang,Bi Yong-Mei,Steven, Rothstein J.,Steven, Rothstein J.,Jan, Leach E.. 2015

[7]Validation of reference genes of grass carp Ctenopharyngodon idellus for the normalization of quantitative real-time PCR. Ye, Xing,Zhang, Lili,Tian, Yuanyuan,Lao, Haihua,Bai, Junjie,Yu, Lingyun,Dong, Haiyan,Dong, Haiyan.

[8]Molecular characterization and expression analysis of Hsp90 in Schizothorax prenanti. Zhu, Jieyao,Wang, Hong,Zhang, Xin,Hao, Jin,Wu, Yuanbin,Li, Zhiqiong,Chen, Defang,Geng, Yi,Wang, Kaiyu,Zhou, Jian,Chen, Defang,Zhou, Jian,Chen, Defang.

[9]Genetic diversity in three Japanese flounder (Paralichthys olivaceus) populations revealed by ISSR markers. Liu, YG,Chen, SL,Li, J,Li, BF. 2006

[10]Molecular identification and expression analysis of natural resistance associated macrophage protein (Nramp) cDNA from Japanese flounder (Paralichthys olivaceus). Chen, SL,Wang, ZJ,Xu, MY,Gui, JF. 2006

[11]Screening and analysis of PoAkirin1 and two related genes in response to immunological stimulants in the Japanese flounder (Paralichthys olivaceus). Yang, Chang-Geng,Sun, Bing,Liu, Shan-Shan,Chen, Song-Lin,Yang, Chang-Geng,Wang, Xian-Li,Zhang, Bo. 2013

[12]Effects of dietary supplementation of A3 alpha-peptidoglycan on innate immune responses and defense activity of Japanese flounder (Paralichthys olivaceus). Zhou, J,Song, XL,Huang, J,Wang, XH. 2006

[13]Production and confirmation of clones using gynogenesis in Japanese flounder. Liu, Haijin,Liu, Yongxin,Wang, Guixing,Wang, Yufen,Si, Fei,Sun, Zhaohui,Zhang, Xiaoyan,Liu, Yi,Hou, Jilun. 2011

[14]Assessing the genetic structure of three Japanese flounder (Paralichthys olivaceus) stocks by microsatellite markers. Liu, YG,Chen, SL,Li, BF. 2005

[15]Major histocompatibility complex class IIB allele polymorphism and its association with resistance/susceptibility to Vibrio anguillarum in Japanese flounder (Paralichthys olivaceus). Zhang, Y. X.,Chen, S. L.,Liu, Y. G.,Sha, Z. X.,Liu, Z. J.. 2006

[16]Production and verification of heterozygous clones in Japanese flounder, Paralichthys olivaceus by microsatellite marker. Liu, Haijin,Liu, Yongxin,Wang, Guixing,Wang, Yufen,Si, Fei,Sun, Zhaohui,Zhang, Xiaoyan,Liu, Yi,Hou, Jilun. 2011

[17]Tissue-specific accumulation of cadmium and its effects on antioxidative responses in Japanese flounder juveniles. Cao, Liang,Huang, Wei,Shan, Xiujuan,Dou, Shuozeng,Huang, Wei,Shan, Xiujuan,Ye, Zhenjiang.

[18]Genome-wide SNP identification for the construction of a high-resolution genetic map of Japanese flounder (Paralichthys olivaceus): applications to QTL mapping of Vibrio anguillarum disease resistance and comparative genomic analysis. Shao, Changwei,Liu, Yang,Wang, Lei,Tian, Yongsheng,Chen, Songlin,Shao, Changwei,Liu, Yang,Wang, Lei,Tian, Yongsheng,Chen, Songlin,Shao, Changwei,Sakamoto, Takashi,Niu, Yongchao,Xie, Zhiyuan,Tai, Shuaishuai,Rastas, Pasi,Li, Hengde,Jiang, Yong.

[19]Genetic verification of doubled haploid Japanese flounder, Paralichthys olivaceus by genotyping telomeric microsatellite loci. Liu, Yongxin,Liu, Haijin,Wang, Guixing,Wang, Yufen,Si, Fei,Sun, Zhaohui,Zhang, Xiaoyan,Liu, Yi,Hou, Jilun. 2012

[20]Efficacy of Vibrio anguillarum antigen administered by intraperitoneal injection route in Japanese flounder, Paralichthys olivaceus (Temminck et Schlegel). Li, J,Gao, DM,Wang, Q,Wang, JY,Wang, QY. 2005

作者其他论文 更多>>

微信小程序