A sandwich dipstick assay for ATP detection based on split aptamer fragments

文献类型： 外文期刊

第一作者： Zhu, Chao

作者： Zhu, Chao;Zhao, Yan;Yan, Mengmeng;Huang, Yafei;Yan, Jiao;Bai, Wenhui;Chen, Ailiang;Zhu, Chao;Zhao, Yan;Yan, Mengmeng;Huang, Yafei;Yan, Jiao;Bai, Wenhui;Chen, Ailiang;Huang, Yafei;Yan, Jiao

作者机构：

关键词： Split aptamer fragments;Dipstick strip;ATP;Gold nanoparticles

期刊名称：ANALYTICAL AND BIOANALYTICAL CHEMISTRY （ 影响因子：4.142； 五年影响因子：3.863 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Aptamer-based strip assay is an easy, highly efficient and low-cost detection method, which has been developed and easily applied to onsite detection. A new sensitive sandwich dipstick assay for adenosine triphosphate (ATP) detection was successfully developed based on specific recognition between split aptamer fragments and the target. In this method, the thiolated aptamer was first conjugated to the surface of gold nanoparticles (AuNPs), while the biotin-aptamer was immobilized on the surface of a nitrocellulose filter in the test line. In the presence of ATP, the thiol-aptamer/ATP/biotin-aptamer complexes were generated, which led to an obvious increase in optical signals at the test line. Under the optimal determination conditions, an excellent linear logarithmic response to the ATP concentration was obtained within the range of 0.5 mu M to 5 mM. The limit of detection (LOD) of 0.5 mu M was reached at a signal-to-noise ratio of 3. The dipstick assay showed a good average recovery of 96-108 % with the RSD of less than 20 % in urine samples. The proposed method exhibited high specificity against other nucleotides such as the uridine triphosphate (UTP), cytidine triphosphate (CTP), and guanosine triphosphate (GTP). The results indicated that the dipstick strip may be considered as an inexpensive screening tool for onsite ATP determination.

分类号： O65

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