Dynamic transcriptome analysis reveals AP2/ERF transcription factors responsible for cold stress in rapeseed (Brassica napus L.)

文献类型： 外文期刊

第一作者： Du, Chunfang

作者： Du, Chunfang;Hu, Kaining;Liu, Chunqing;Tu, Jinxing;Fu, Tingdong;Du, Chunfang;Xian, Shuanshi;Fan, Jianchun

作者机构：

关键词： Transcriptome analysis;AP2/ERF transcription factors;B. napus;Stress response;Brassicaceae

期刊名称：MOLECULAR GENETICS AND GENOMICS （ 影响因子：3.291； 五年影响因子：3.257 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： The APETALA2/ethylene response factor (AP2/ERF) transcription factor (TF) superfamily plays an important regulatory role in signal transduction of the plant responses to various stresses including low temperature. Significant progress has been made in understanding the mechanism of cold resistance in Brassica napus, an important oilseed crop. However, comprehensive studies on the induction and activity of these TFs under low temperature have been lacking. In this study, 132 AP2/ERF genes were identified by transcriptome sequencing of rapeseed leaves exposed to 0, 2, 6, 12, and 24 h of low (4 A degrees C) temperature stress. The genes were classified into 4 subfamilies (AP2, DREB, ERF, and RAV) and 13 subgroups, among which the DREB subfamily and ERF subfamily contained 114 genes, no genes were assigned to soloist or DREB A3 subgroups. One hundred and eighteen genes were located on chromosomes A1 to C9. GO functional analysis and promoter sequence analysis revealed that these genes are involved in many molecular pathways that may enhance cold resistance in plants, such as the low-temperature responsiveness, methyl jasmonate, abscisic acid, and ethylene-responsiveness pathways. Their expression patterns revealed dynamic control at different times following initiation of cold stress; the RAV and DREB subfamilies were expressed at the early stage of cold stress, whereas the AP2 subfamily was expressed later. Quantitative PCR analyses of 13 cold-induced AP2/ERF TFs confirmed the accuracy of above results. This study is the first dynamic analysis of the AP2/ERF TFs responsible for cold stress in rapeseed. These findings will serve as a reference for future functional research on transcription in rapeseed.

分类号： Q7

  • 相关文献

[1]Association Mapping of Flowering Time QTLs and Insight into Their Contributions to Rapeseed Growth Habits. Wang, Nian,Chen, Biyun,Xu, Kun,Gao, Guizhen,Li, Feng,Qiao, Jiangwei,Yan, Guixin,Li, Jun,Li, Hao,Wu, Xiaoming,Wang, Nian. 2016

[2]BnEPFL6, an EPIDERMAL PATTERNING FACTOR-LIKE (EPFL) secreted peptide gene, is required for filament elongation in Brassica napus. Huang, Yi,Tao, Zhangsheng,Liu, Qiong,Wang, Xinfa,Yu, Jingyin,Liu, Guihua,Wang, Hanzhong.

[3]Salt tolerance conferred by expression of a global regulator IrrE from Deinococcus radiodurans in oilseed rape. Wang, Jin,Lin, Min,Guo, Cui,Dai, Qilin,Feng, Bin,Zuo, Kaijing.

[4]Selection and evaluation of novel reference genes for quantitative reverse transcription PCR (qRT-PCR) based on genome and transcriptome data in Brassica napus L.. Yang, Hongli,Liu, Jing,Huang, Shunmou,Deng, Linbin,Hua, Wei,Guo, Tingting.

[5]Widespread and evolutionary analysis of a MITE family Monkey King in Brassicaceae. Dai, Shutao,Hou, Jinna,Long, Yan,Wang, Jing,Li, Cong,Xiao, Qinqin,Jiang, Xiaoxue,Zou, Xiaoxiao,Zou, Jun,Meng, Jinling,Hou, Jinna. 2015

[6]The complete chloroplast genome of Arabidopsis lyrata. Wu, Zhiqiang,Gu, Cuihua,Tembrock, Luke R.,Sun, Cheng. 2016

[7]Estimating Plasmodiophora brassicae gene expression in lines of B. rapa by RT-PCR. Wu, Li-yan,Li, Shi-kai,Gong, Ya-ju,Zhong, Li,He, Jiang-Ming,Siemens, Johannes,Ludwig-Mueller, Jutta. 2012

[8]Detection of Contarinia nasturtii (Diptera : Cecidomyiidae) in New York, a new pest of cruciferous plants in the United States. Kikkert, Julie R.,Hoepting, Christine A.,Wu, Qingjun,Wang, Ping,Baur, Robert,Shelton, Anthony M..

[9]Nematode resistance of rape-radish chromosome addition lines. Peterka, Herbert,Budahn, Holger,Zhang, Shao Song,Li, Jin Bin. 2010

[10]Lineage-specific evolution of Methylthioalkylmalate synthases (MAMs) involved in glucosinolates biosynthesis. Zhang, Jifang,Wang, Xiaobo,Cheng, Feng,Wu, Jian,Liang, Jianli,Wang, Xiaowu,Zhang, Jifang,Yang, Wencai. 2015

[11]A 36-bp deletion in the alpha subunit of glutamate-gated chloride channel contributes to abamectin resistance in Plutella xylostella. Liu, Feng,Shi, Xiuzhen,Wu, Qingjun,Xu, Baoyun,Xie, Wen,Wang, Shaoli,Zhang, Youjun,Liang, Yanpo,Liu, Feng,Liu, Nannan.

[12]Syntenic gene analysis between Brassica rapa and other Brassicaceae species. Cheng, Feng,Wu, Jian,Fang, Lu,Wang, Xiaowu. 2012

[13]Glucosinolates in Chinese Brassica campestris vegetables: Chinese cabbage, purple cai-tai, choysum, pakehoi, and turnip. Chen, Xinjuan,Zhu, Zhujun,Zhu, Zhujun,Chen, Xinjuan,Gerendas, Joska,Zimmermann, Nadine. 2008

[14]HETEROLOGOUS EXPRESSION OF AN ALLIGATORWEED HIGH-AFFINITY POTASSIUM TRANSPORTER GENE ENHANCES SALINITY TOLERANCE IN ARABIDOPSIS THALIANA. Song, Zhizhong,Yang, Shunying,Jin, Man,Su, Yanhua,Song, Zhizhong,Yang, Shunying,Jin, Man,Zhu, Hong,Zhu, Hong. 2014

[15]Genome-wide identification, classification, and analysis of NADP-ME family members from 12 crucifer species. Tao, Peng,Li, Biyuan,Wang, Wuhong,Yue, Zhichen,Lei, Juanli,Zhao, Yanting,Zhong, Xinmin,Guo, Weiling.

[16]Temperature-Dependent Survival of Turnip Crinkle Virus-Infected Arabidopsis Plants Relies on an RNA Silencing-Based Defense That Requires DCL2, AG02, and HEN1. Zhang, Xiuchun,Zhang, Xiaofeng,Singh, Jasleen,Qu, Feng,Zhang, Xiuchun,Zhang, Xiaofeng,Li, Dawei.

[17]Myrosinases from root and leaves of Arabidopsis thaliana have different catalytic properties. Andersson, Derek,Bejai, Sarosh,Meijer, Johan,Chakrabarty, Romit,Zhang, Jiaming,Rask, Lars.

[18]Genetic and cytological analysis of a new spontaneous male sterility in radish (Raphanus sativus L.). Wang, Zhi-Wei,Gao, Lei,Zhou, Yuan,Wang, Ting,Wang, Zhi-Wei,Xiang, Chang Ping,Liu, Hai Zhou,Mei, Shi Yong.

[19]Comparative transcriptome analysis of cotton fiber development of Upland cotton (Gossypium hirsutum) and Chromosome Segment Substitution Lines from G. hirsutum x G. barbadense. Peng-tao Li,Mi Wang,Guo, Xiao-ping,Shi, Yu-zhen,Yuan, You-lu,Quan-wei Lu,Qun Ge,Md. Harun or Rashid,Ai-ying Liu,Ju-wu Gong,Hai-hong Shang,Wan-kui Gong,Jun-wen Li,Wei-wu Song,Li-xue Guo,Wei Su,Shao-qi Li,Xiao-ping Guo,Yu-zhen Shi,You-lu Yuan. 2017

[20]Analysis of sea-island cotton and upland cotton in response to Verticillium dahliae infection by RNA sequencing. Quan Sun,Huaizhong Jiang,Xiaoyan Zhu,Weina Wang,Xiaohong He,Yuzhen Shi,Youlu Yuan,Xiongming Du,Yingfan Cai. 2013

作者其他论文 更多>>

微信小程序