Cloning and expression analysis of c-type lysozyme gene in golden pompano, Trachinotus ovatus

文献类型： 外文期刊

第一作者： Zhou, Chuanpeng

作者： Zhou, Chuanpeng;Lin, Heizhao;Huang, Zhong;Wang, Jun;Wang, Yun;Yu, Wei;Zhou, Chuanpeng;Lin, Heizhao;Huang, Zhong;Yu, Wei

作者机构：

关键词： Trachinotusovatus;Innate immune;c-type lysozyme gene;Vibrio harveyi

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： It is well known that lysozymes are key proteins to teleosts in the innate immune system and possess high bactericidal properties. In the present study, a c-type lysozyme gene (To-lysC) was cloned from golden pompano, Trachinotus ovatus. The To-lysC cDNA is composed of 743 bp with a 36 bp of 5'-UTR, 432 bp open reading frame (ORF) and 275 bp 3'-UTR, encoding a polypeptide of 144 amino acids (GenBank accession no: KT935522). Phylogenetic analysis revealed that To-lysC showed highest similarity to Perca flavescens lysC. Quantitative real-time PCR (qRT-PCR) analysis showed that To-lysC had relatively high expression level in the head kidney, gill and brain. After Vibrio harveyi infection, transcripts of To-lysC increased and reached its peak at 12 h p.i. These results indicated that To-lysC may play an important role in innate immune response to bacteria. (C) 2016 Elsevier Ltd. All rights reserved.

分类号： S9

  • 相关文献

[1]Molecular cloning and mRNA expression of a hepcidin gene from the spinyhead croaker, Collichthys lucidus. Sang, C.,Lin, Y.,Jiang, K.,Zhang, F.,Song, W.. 2015

[2]The characterization, expression and activity analysis of superoxide dismutases (SODs) from Procambarus clarkii. Meng, Qingguo,Chen, Jing,Xu, Chenchao,Wang, Ya,Wang, Tingting,Zhai, Xiaoting,Gu, Wei,Wang, Wen,Meng, Qingguo,Chen, Jing,Xu, Chenchao,Wang, Ya,Wang, Tingting,Zhai, Xiaoting,Gu, Wei,Wang, Wen,Huang, Yanqing. 2013

[3]Effects of repeated handling and air exposure on the immune response and the disease resistance of gibel carp (Carassius auratus gibelio) over winter. Yang, Bingyuan,Tu, Yongqin,Hu, Huihua,Han, Dong,Zhu, Xiaoming,Jin, Junyan,Yang, Yunxia,Xie, Shouqi,Yang, Bingyuan,Tu, Yongqin,Hu, Huihua,Wang, Cuicui,Han, Dong.

[4]THE ENDOPARASITOID Campoletis chlorideae INDUCES A HEMOLYTIC FACTOR IN THE HERBIVOROUS INSECT Helicoverpa armigera. Bai, Su-Fen,Li, Xin,Yin, Xin-Ming,Li, Xian-Chun,Li, Xian-Chun,Li, Xian-Chun.

[5]Repeated handling compromises the immune suppression and improves the disease resistance in overwintering channel catfish (Ictalurus punctatus). Yang, Bingyuan,Hu, Huihua,Tu, Yongqin,Han, Dong,Zhu, Xiaoming,Jin, Junyan,Yang, Yunxia,Xie, Shouqi,Yang, Bingyuan,Hu, Huihua,Tu, Yongqin,Wang, Cuicui,Han, Dong.

[6]Effects of dietary hawthorn extract on growth performance, immune responses, growth- and immune-related genes expression of juvenile golden pompano (Trachinotus ovatus) and its susceptibility to Vibrio harveyi infection. Tan, Xiaohong,Huang, Zhong,Zhou, Chuanpeng,Lin, Heizhao,Tan, Lianjie,Xun, Pengwei,Huang, Qian,Tan, Xiaohong,Sun, Zhenzhu,Huang, Zhong,Lin, Heizhao. 2017

[7]Effects of dietary dandelion extracts on growth performance, body composition, plasma biochemical parameters, immune responses and disease resistance of juvenile golden pompano Trachinotus ovatus. Tan, Xiaohong,Sun, Zhenzhu,Chen, Shu,Chen, Silin,Zou, Cuiyun,Liu, Qingying,Ye, Huaqun,Ye, Chaoxia,Wang, Anli,Tan, Xiaohong,Huang, Zhong,Zhou, Chuanpeng,Lin, Heizhao,Huang, Zhong,Lin, Heizhao.

[8]Effect of Dietary Marine Red Yeast Rhodotorula mucilaginosa on the Growth Performance, and also Non-Specific Immune Responses of Juvenile Golden Pompano Trachinotus Ovatus when Challenged with Vibrio Harveyi. Zhou, Chuanpeng,Lin, Heizhao,Xia, Dongmei,Yang, Keng,Yang, Yingying,Huang, Zhong,WeiYu,Zhou, Chuanpeng,Lin, Heizhao,Xia, Dongmei,Yang, Keng,Yang, Yingying,Huang, Zhong,WeiYu,Lin, Heizhao,Huang, Zhong,WeiYu. 2016

[9]Isolation, identification and pathogenicity of Vibrio harveyi, the causal agent of skin ulcer disease in juvenile hybrid groupers Epinephelus fuscoguttatus x Epinephelus lanceolatus. Shen, G. M.,Shi, C. Y.,Fan, C.,Jia, D.,Wang, S. Q.,Xie, G. S.,Li, G. Y.,Mo, Z. L.,Huang, J.,Shen, G. M.,Shi, C. Y.,Fan, C.,Jia, D.,Wang, S. Q.,Xie, G. S.,Li, G. Y.,Mo, Z. L.,Huang, J.,Shen, G. M.,Fan, C.,Jia, D..

[10]The Effects of dietary soybean isoflavones on growth, innate immune responses, hepatic antioxidant abilities and disease resistance of juvenile golden pompano Trachinotus ovatus. Zhou, Chuanpeng,Lin, Heizhao,Niu, Jin,Wang, Jun,Wang, Yun,Chen, Lixiong,Huang, Zhong,Yu, Wei,Tan, Xiaohong,Zhou, Chuanpeng,Ge, Xianping.

[11]Molecular cloning and expression analysis of major histocompatibility complex class IIB gene of the Whitespotted bambooshark (Chiloscyllium plagiosum). Ma, Qian,Zhuang, Zhi-Meng,Tang, Qi-Sheng,Ma, Qian,Su, Yong-Quan,Wang, Jun.

作者其他论文 更多>>

微信小程序