Biological evaluation of N-2-hydroxypropyl trimethyl ammonium chloride chitosan as a carrier for the delivery of live Newcastle disease vaccine

文献类型： 外文期刊

第一作者： Zhao, Kai

作者： Zhao, Kai;Wang, Xiaohua;Zhao, Kai;Sun, Yanwei;Chen, Gang;Rong, Guangyu;Kang, Hong;Wang, Xiaohua;Sun, Yanwei;Jin, Zheng

作者机构：

关键词： Newcastle disease;Live Newcastle disease vaccine;N-2-hydroxypropyl trimethyl ammonium chloride;Chitosan nanoparticles;Mucosal immune delivery system;Immune efficacy

期刊名称：CARBOHYDRATE POLYMERS （ 影响因子：9.381； 五年影响因子：8.678 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： Mucosal immune system plays a very important role in antiviral immune response. We prepared New-castle disease viruses (NDV) encapsulated in N-2-hydroxypropyl trimethyl ammonium chloride chitosan (N-2-HACC) nanoparticles (NDV/La Sota-N-2-HACC-NPs) by an ionic cross linking method, and assessed the potential of N-2-HACC-NPs as a mucosal immune delivery carrier. The properties of the nanoparticles were determined by transmission electron microscopy, Zeta potential and particle size analysis, encapsulation efficiency and loading capacity. NDV/La Sota-N-2-HACC-NPs have regular spherical morphologies and high stability; with 303.88 +/- 49.8 nm mean diameter, 45.77 +/- 0.75 mV Zeta potential, 94.26 +/- 0.42% encapsulation efficiency and 54.06 +/- 0.21% loading capacity. In vitro release assay indicated that the release of NDV from NDV/La Sota-N-2-HACC-NPs is slow. The NDV/La Sota-N-2-HACC-NPs have good biological characteristics, very low toxicity and high level of safety. Additionally, specific pathogen-free chickens immunized with NDV/La Sota-N-2-HACC-NPs showed much stronger cellular, humoral and mucosal immune responses than commercial attenuated live Newcastle disease vaccine, and NDV/La SotaN-2-HACC-NPs reached the sustainable release effect. Our study here provides a foundation for the further development of mucosal vaccines and drugs, and the N-2-HACC-NPs should be a potential drug delivery carrier with immense potential in medical applications. (C) 2016 Elsevier Ltd. All rights reserved.

分类号： O629`Q53

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