Characterization of the Methylation Status of Pax7 and Myogenic Regulator Factors in Cell Myogenic Differentiation

文献类型： 外文期刊

第一作者： Chao, Zhe

作者： Chao, Zhe;Zheng, Xin-Li;Sun, Rui-Ping;Liu, Hai-Long;Huang, Li-Li;Cao, Zong-Xi;Wang, Feng;Deng, Chang-Yan

作者机构：

关键词： Pax7;Myogenic Regulatory Factors;C2C12;DNA Methylation;CpG Islands

期刊名称：ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES （ 影响因子：2.509； 五年影响因子：2.604 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Epigenetic processes in the development of skeletal muscle have been appreciated for over a decade. DNA methylation is a major epigenetic modification important for regulating gene expression and suppressing spurious transcription. Up to now, the importance of epigenetic marks in the regulation of Pax7 and myogenic regulatory factors (MRFs) expression is far less explored. In the present study, semi-quantitative the real-time polymerase chain reaction (RT-PCR) analyses showed MyoD and Myf5 were expressed in activated and quiescent C2C12 cells. MyoG was expressed in a later stage of myogenesis. Pax7 was weakly expressed in differentiated C2C12 cells. To further understand the regulation of expression of these genes, the DNA methylation status of Pax7, MyoD, and Myf5 was determined by bisulfite sequencing PCR. During the C2C12 myoblasts fusion process, the changes of promoter and exon 1 methylation of Pax7, MyoD, and Myf5 genes were observed. In addition, an inverse relationship of low methylation and high expression was found. These results suggest that DNA methylation may be an important mechanism regulating Pax7 and MRFs transcription in cell myogenic differentiation.

分类号： Q95

  • 相关文献

[1]Paired box 7 (Pax7) gene: molecular characterisation, polymorphism and its association with growth performance in goose (Anser cygnoides). Wang, C.,Yang, Y. Z.,Liu, Y.,Gong, S. M.,Wang, H. Y.,He, D. Q..

[2]MicroRNA-95 promotes myogenic differentiation by down-regulation of aminoacyl-tRNA synthase complex-interacting multifunctional protein 2. Li, Biao,Xie, Shanshan,Cai, Chunbo,Qian, Lili,Jiang, Shengwang,Ma, Dezun,Xiao, Gaojun,Gao, Ting,Cui, Wentao,Yang, Jinzeng. 2017

[3]CLONING OF BASIC FIBROBLAST GROWTH FACTOR FROM CHINESE SMALL TAIL HAN SHEEP AND ITS EFFECTS ON PROLIFERATION OF MURINE C2C12 MYOBLASTS. Liu, Hua-zhong,Chen, Shao-hong,Luo, Ping,Shang, Jiang-hua. 2013

[4]Rapid alterations of gene expression and cytosine methylation in newly synthesized Brassica napus allopolyploids. Xu, Yanhao,Zhong, Lan,Wang, Jianbo,Wu, Xiaoming,Fang, Xiaoping.

[5]Widespread and evolutionary analysis of a MITE family Monkey King in Brassicaceae. Dai, Shutao,Hou, Jinna,Long, Yan,Wang, Jing,Li, Cong,Xiao, Qinqin,Jiang, Xiaoxue,Zou, Xiaoxiao,Zou, Jun,Meng, Jinling,Hou, Jinna. 2015

[6]Genome-wide high-resolution mapping of DNA methylation identifies epigenetic variation across embryo and endosperm in Maize (Zea may). Wang, Pengfei,Ma, Chuanxi,Wang, Pengfei,Xia, Han,Zhang, Ye,Zhao, Shuzhen,Zhao, Chuanzhi,Hou, Lei,Li, Changsheng,Li, Aiqin,Wang, Xingjun. 2015

[7]Synonymous Codon Usage Bias in the Plastid Genome is Unrelated to Gene Structure and Shows Evolutionary Heterogeneity. Qi, Yueying,Xu, Wenjing,Xing, Tian,Zhao, Mingming,Yan, Li,Xia, Guangmin,Wang, Mengcheng,Li, Nana. 2015

[8]Alteration of the DNA methylation status of donor cells impairs the developmental competence of porcine cloned embryos. Huan, Yan Jun,Zhu, Jiang,Xie, Bing Teng,Wang, Jian Yu,Li, Jing Yu,Xue, Bing Hua,Kong, Qing Ran,Liu, Zhong Hua,Huan, Yan Jun,Wu, Zhan Feng,Zhang, Ji Guang.

[9]Study on DNA Cytosine Methylation of Cotton (Gossypium hirsutum L.) Genome and Its Implication for Salt Tolerance. ZHAO Yun-lei,YU Shu-xun,YE Wu-wei,WANG Hong-mei,WANG Jun-juan,FANG Bao-xing. 2010

[10]Induced Pib Expression and Resistance to Magnaporthe grisea are Compromised by Cytosine Demethylation at Critical Promoter Regions in Rice. Li, Yuan,Xia, Qiong,Wu, Ying,Xu, Chunming,Liu, Bao,Li, Yuan,Xia, Qiong,Wu, Ying,Xu, Chunming,Liu, Bao,Kou, Hongping,Wang, Dan,Lin, Xiuyun,Xing, Shaochen. 2011

[11]Effect of different light quality on DNA methylation variation for brown cotton (Gossypium hirstum). Li, Tingchun,Fan, Honghong,Li, Zhengpeng,Wei, Jun,Cai, Yongping,Lin, Yi,Li, Tingchun. 2011

[12]Laser Irradiation-Induced DNA Methylation Changes Are Heritable and Accompanied with Transpositional Activation of mPing in Rice. Li, Siyuan,Xia, Qiong,Yu, Xiaoming,Gao, Xiang,Liu, Bao,Li, Siyuan,Wang, Fang,Ma, Jian,Kou, Hongping,Lin, Xiuyun. 2017

[13]Performances of Different Fragment Sizes for Reduced Representation Bisulfite Sequencing in Pigs. Yuan, Xiao-Long,Zhang, Zhe,Pan, Rong-Yang,Gao, Ning,Deng, Xi,Li, Bin,Zhang, Hao,Li, Jia-Qi,Yuan, Xiao-Long,Sangild, Per Torp,Gao, Ning. 2017

[14]Molecular characterizations of DNA methyltransferase 3 and its roles in temperature tolerance in the whitefly, Bemisia tabaci Mediterranean. Hong, X. -Y.,Dai, T. -M,Lu, Z. -C.,Wang, Y. -S.,Liu, W. -X.,Wan, F. -H.,Wan, F. -H.. 2018

[15]Promoter methylation negatively correlated with mRNA expression but not tissue differential expression after heat stress. Gan, J. K.,Zhang, D. X.,Zhang, X. Q.,Chen, Z. Y.,Luo, Q. B.,Gan, J. K.,Zhang, D. X.,He, D. L.,Zhang, X. Q.,Chen, Z. Y.,Luo, Q. B.,He, D. L.. 2013

[16]Mutation of the RDR1 gene caused genome-wide changes in gene expression, regional variation in small RNA clusters and localized alteration in DNA methylation in rice. Wang, Ningning,Zhang, Di,Wang, Zhenhui,Xun, Hongwei,Wang, Hui,Huang, Wei,Liu, Ying,Li, Ning,Ou, Xiufang,Zhang, Chunyu,Liu, Bao,Wang, Ningning,Ma, Jian,Lin, Xiuyun,Zhang, Chunyu,Wang, Ningning,Wang, Ming-Bo. 2014

[17]A Comparative Analysis of DNA Methylation in Diploid and Tetraploid Apple (Malus x domestica Borkh.). He, Ping,Cheng, Lailiang,Li, Huifeng,Wang, Haibo,Li, Linguang. 2017

[18]Comparative analysis of DNA methylation changes in two rice genotypes under salt stress and subsequent recovery. Wang, Wensheng,Huang, Fei,Qin, Qiao,Zhao, Xiuqin,Li, Zhikang,Fu, Binying,Li, Zhikang,Fu, Binying.

[19]Expression and methylation of microsomal triglyceride transfer protein and acetyl-CoA carboxylase are associated with fatty liver syndrome in chicken. Liu, Zhen,Li, Qinghe,Liu, Ranran,Zhao, Guiping,Zhang, Yonghong,Zheng, Maiqing,Cui, Huanxian,Li, Peng,Cui, Xiaoyan,Liu, Jie,Wen, Jie,Liu, Zhen,Li, Qinghe,Liu, Ranran,Zhao, Guiping,Zheng, Maiqing,Cui, Huanxian,Li, Peng,Cui, Xiaoyan,Liu, Jie,Wen, Jie,Zhang, Yonghong.

[20]Polymorphisms and DNA methylation level in the CpG site of the GHR1 gene associated with mRNA expression, growth traits and hormone level of half-smooth tongue sole (Cynoglossus semilaevis). Zhao, J. L.,Si, Y. F.,He, F.,Wen, H. S.,Li, J. F.,Ren, Y. Y.,Zhao, M. L.,Huang, Z. J.,Zhao, J. L.,Si, Y. F.,He, F.,Wen, H. S.,Li, J. F.,Ren, Y. Y.,Zhao, M. L.,Huang, Z. J.,Chen, S. L..

作者其他论文 更多>>

微信小程序