Rapid Discrimination between Groups I and II of Acidovorax citrulli Using a Primer Pair Specific to a pilL Gene

文献类型： 外文期刊

第一作者： Zhong, Jie

作者： Zhong, Jie;Lin, Zhen-Ya;Ma, Ya-Min;Gao, Bi-Da;Liu, Hui-Qin;Zhao, Ting-Chang;Schaad, Norman W.

作者机构：

关键词： Acidovorax citrulli;group I(II);primers

期刊名称：JOURNAL OF PHYTOPATHOLOGY （ 影响因子：1.789； 五年影响因子：1.574 ）

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收录情况： SCI

摘要： Acidovorax citrulli can be divided into two genetic groups: group I and group II based primarily on pulsed-field gel electrophoresis (PFGE) and multilocus sequence classification (MLST). To distinguish more rapidly between strains of the two groups, a pair of specific primer for specific polymerase chain reaction (PCR) that can identify group II strains was designed based on the pilL gene of a group II strain, AAC00-1. PCR results showed that a 332-bp band was generated for 51 of 52 group II strains whereas only three of 93 group I strains were positive, largely consisting with previous studies of A.citrulli classification. Results of PCR showed the primers were able to detect group II strains of A.citrulli and distinguish between strains of groups I and II rapidly and accurately.

分类号： Q945

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