An optimized, highly efficient, self-assembled, subvirus-like particle of infectious bursal disease virus (IBDV)
文献类型: 外文期刊
第一作者: Wang, Miao
作者: Wang, Miao;Pan, Qing;Lu, Zhen;Li, Kai;Gao, Honglei;Qi, Xiaole;Gao, Yulong;Wang, Xiaomei;Wang, Xiaomei
作者机构:
关键词: Infectious bursal disease virus;Yeast;sVP
期刊名称:VACCINE ( 影响因子:3.641; 五年影响因子:3.816 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: Infectious bursal disease virus (IBDV) causes immunosuppression in young chickens, leading to increased susceptibility to other diseases and a reduction in the immune response to other vaccines. Thus, IBDV results in great economic losses to the poultry industry. The most effective method of prevention is vaccination. However, medium-virulence vaccines can cause bursal pathological damage and immunosuppression. Here, we describe a safer, self-assembled, subvirus-like particle (sVP) vaccine without a complex purification process. The IBD-VP2 gene was cloned into Pichia pastoris, and the expressed protein self-assembled into T=1 sVPs (similar to 23 nm). Immunization experiments showed that the sVP vaccine elicited high IBDV-neutralizing antibodies in each group, and all birds survived challenge with very virulent IBDV (vvIBDV). Additionally, IBDV RNA was not detected, and sterile immunity was achieved. In conclusion, the IBD-sVP is a suitable candidate for a recombinant subunit vaccine against IBDV. (C) 2016 Published by Elsevier Ltd.
分类号: R392
- 相关文献
作者其他论文 更多>>
-
A multiple-population study reveals genetic variation and genes associated with resistance to Streptococcus agalactiae in Nile tilapia (Oreochromis niloticus)
作者:Wang, Zhang;Liu, Zhigang;Huang, Shaowei;Zhu, Weijuan;Cao, Jianmeng;Wang, Miao;Yi, Mengmeng;Lu, Maixin;Ke, Xiaoli;Liu, Zhigang;Huang, Shaowei;Zhu, Weijuan;Lu, Maixin;Ke, Xiaoli
关键词:Tilapia; Streptococcus agalactiae; GWAS; Selective sweep analysis; SNP marker
-
Mechanisms of Baicalin Alleviates Intestinal Inflammation: Role of M1 Macrophage Polarization and Lactobacillus amylovorus
作者:Zhang, Shunfen;Zhong, Ruqing;Li, Kai;Wang, Huixin;Xu, Ye;Liu, Dadan;Chen, Liang;Zhang, Hongfu;Zhang, Shunfen;Lv, Huiyuan;Ma, Qiugang;Zhou, Miao
关键词:baicalin;
E. coli ; intestinal inflammation;Lactobacillus amylovorus ; macrophages polarization; TLR4 -
Insight into the mechanism of efficient extraction of tea saponins from Camellia oleifera shells using deep eutectic solvents
作者:Mo, Xiangyan;Hang, Fangxue;Ren, Er-Fang;Gai, Lili;Li, Kai;Niu, Debao;Ren, Er-Fang
关键词:Camellia oleifera shells; Deep eutectic solvent; Tea saponins; UPLC-Q-Orbitrap HRMS; Molecular dynamic simulation; Extraction mechanism
-
Marek's disease virus protein kinase US3 inhibits DNA-sensing antiviral innate immunity via abrogating activation of NF-κB
作者:Li, Kai;Liu, Rui;Liu, Yongzhen;Gao, Li;Liu, Changjun;Zhang, Yanping;Qi, Xiaole;Cui, Hongyu;Wang, Suyan;Chen, Yuntong;Duan, Yulu;Gao, Yulong;Wang, Xiaomei;Li, Kai;Li, Kai;Wang, Xiaomei
关键词:Marek's disease virus; US3; innate immunity; DNA sensing; NF-kappa B
-
Extraction Technology, Component Analysis, and Biological Activity of Essential Oils from Ligusticum jeholense Nakai et Kitag. Vegetables
作者:Wang, Miao;Zheng, Qiang;Bai, Xia;Li, Donghua;Li, Jiangkuo;Xu, Yamin;Liu, Pengyu
关键词:Ligusticum jeholense Nakai et Kitag.; essential oil; response surface methodology; antioxidant; antimicrobial activity; GC-MS
-
Evaluation and Validation of Colloidal Gold Immunochromatographic Qualitative Testing Products for the Detection of Emamectin Benzoate, Isocarbophos, and Fipronil in Cowpea Samples
作者:Song, Anning;Wang, Miao;She, Yongxin;Jin, Maojun;Cao, Zhen;Wang, Jing;Wang, Miao;She, Yongxin;Jin, Maojun;Cao, Zhen;Wang, Jing;Abd El-Aty, A. M.;Abd El-Aty, A. M.
关键词:food safety; colloidal gold immunochromatographic qualitative testing; cowpea; pesticide residue; emamectin benzoate; isocarbophos; fipronil
-
Long-Term Crop Rotation Revealed the Relationship Between Soil Organic Carbon Physical Fraction and Bacterial Community at Aggregate Scales
作者:Meng, Xianghai;Wang, Baicheng;Zhang, Xingzhe;Liu, Chunguang;Yang, Bing;Wang, Wenhui;Xu, Dehai;Zhang, Shuai;Wang, Xiaomei;Ji, Jinghong;Hao, Xiaoyu;Cao, Minghui;Wang, Yuming;Wang, Yuming
关键词:aggregate; bacterial community; long-term crop rotation; soil organic carbon