Safety and immunogenicity of a gE/gI/TK gene-deleted pseudorabies virus variant expressing the E2 protein of classical swine fever virus in pigs

文献类型： 外文期刊

第一作者： Lei, Jian-Lin

作者： Lei, Jian-Lin;Xia, Shui-Li;Wang, Yimin;Du, Mingliang;Xiang, Guang-Tao;Cong, Xin;Luo, Yuzi;Li, Lian-Feng;Zhang, Lingkai;Yu, Jiahui;Qiu, Hua-Ji;Sun, Yuan;Lei, Jian-Lin;Hu, Yonghao

作者机构：

关键词： Pseudorabies virus variant;Classical swine fever;Bivalent vaccine;Safety;Immunogenicity

期刊名称：IMMUNOLOGY LETTERS （ 影响因子：3.685； 五年影响因子：3.54 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Classical swine fever (CSF) and pseudorabies (PR) are both major infectious diseases of pigs, causing enormous economic losses to the swine industry in many countries. A marker vaccine that enables differentiation of infected from vaccinated animals (DIVA) is highly desirable for control and eradication of these two diseases in endemic areas. Since late 2011, PR outbreaks have been frequently reported in many Bartha-K61-vaccinated pig farms in China. It has been demonstrated that a pseudorabies virus (PRV) variant with altered antigenicity and increased pathogenicity was responsible for the outbreaks. Previously, we showed that rPRVTJ-delgE/gI/TK, a gE/gl/TK-deleted PRV variant, was safe for susceptible animals and provided a complete protection against lethal PRV variant challenge, indicating that rPRVTJ-delgE/gI/TK can be used as an attractive vaccine vector. To develop a safe bivalent vaccine against CSF and PR, we generated a recombinant virus rPRVTJ-delgE/gI/TK-E2 expressing the E2 protein of classical swine fever virus (CSFV) based on rPRVTJ-delgE/gI/TK and evaluated its safety and immunogenicity in pigs. The results indicated that pigs (n=5) immunized with rPRVTJ-delgE/gI/TK-E2 of different doses did not exhibit clinical signs or viral shedding following immunization, the immunized pigs produced anti-PRV or anti-CSFV neutralizing antibodies and the pigs immunized with 10(6) or 10(6) TCID50 rPRVTJ-delgE/gl/TK-E2 were completely protected against the lethal challenge with either CSFV Shimen strain or variant PRV TJ strain. These findings suggest that rPRVTJ-delgE/gI/TK-E2 is a promising bivalent DIVA vaccine candidate against CSFV and PRV coinfections. (C) 2016 European Federation of Immunological Societies. Published by Elsevier B.V. All rights reserved.

分类号： R392

  • 相关文献

[1]Comprehensive evaluation of the adenovirus/alphavirus-replicon chimeric vector-based vaccine rAdV-SFV-E2 against classical swine fever. Qiu, Hua-Ji. 2013

[2]A safe and molecular-tagged Brucella canis ghosts confer protection against virulent challenge in mice. Qian, Jing,Bu, Zhaoyang,Lang, Xulong,Wang, Xinglong,Qian, Jing,Qian, Jing,Yan, Guangmou,Yang, Yanling,Wang, Xiuran.

[3]A novel gE-deleted pseudorabies virus (PRV) provides rapid and complete protection from lethal challenge with the PRV variant emerging in Bartha-K61-vaccinated swine population in China. Wang, Chun-Hua,Yuan, Jin,Qin, Hua-Yang,Luo, Yuzi,Cong, Xin,Li, Yongfeng,Chen, Jianing,Li, Su,Sun, Yuan,Qiu, Hua-Ji.

[4]Variations in glycoprotein B contribute to immunogenic difference between PRV variant JS-2012 and Bartha-K61. Yu, Zhi-qing,Tong, Wu,Zheng, Hao,Li, Li-wei,Li, Guo-xin,Gao, Fei,Wang, Tao,Liang, Chao,Ye, Chao,Wu, Ji-qiang,Huang, Qinfeng,Tong, Guang-zhi,Tong, Wu,Zheng, Hao,Li, Li-wei,Li, Guo-xin,Gao, Fei,Huang, Qinfeng,Tong, Guang-zhi.

[5]Dose-dependent pathogenicity of a pseudorabies virus variant in pigs inoculated via intranasal route. Wang, Yimin,Xia, Shui-Li,Lei, Jian-Lin,Cong, Xin,Xiang, Guang-Tao,Luo, Yuzi,Sun, Yuan,Qiu, Hua-Ji.

[6]Recombinant Bivalent Vaccine against Foot-and-Mouth Disease Virus Serotype O/A Infection in Guinea Pig. Yi, JZ,Liu, MQ,Zhu, CZ,Zhang, Q,Sheng, ZT,Du, QY,Yan, WY,Zheng, ZX.

[7]Inactivated Recombinant Rabies Viruses Displaying Canine Distemper Virus Glycoproteins Induce Protective Immunity against Both Pathogens. Budaszewski, Renata da Fontoura,Budaszewski, Renata da Fontoura,Sawatsky, Bevan,Kraemer, Beate,von Messling, Veronika,Sawatsky, Bevan,von Messling, Veronika,Hudacek, Andrew,Yin, Xiangping,Schnell, Matthias J.,Yin, Xiangping.

[8]Secreted Expression of the Cap Gene of Porcine Circovirus Type 2 in Classical Swine Fever Virus C-Strain: Potential of C-Strain Used as a Vaccine Vector. Zhang, Lingkai,Li, Yongfeng,Xie, Libao,Wang, Xiao,Sun, Yuan,Qiu, Hua-Ji,Gao, Xulei. 2017

[9]Classical swine fever in China: A minireview. Luo, Yuzi,Li, Su,Sun, Yuan,Qiu, Hua-Ji.

[10]Enhancement of the immunogenicity of an alphavirus replicon-based DNA vaccine against classical swine fever by electroporation and coinjection with a plasmid expressing porcine interleukin 2. Tian, Da-Yong,Sun, Yuan,Meng, Qi-Lin,Wang, Nan,Han, Wen,Li, Su,Li, Yong-Feng,Li, Dan,Ling, Li-Jun,Liao, Ya-Jin,Qiu, Hua-Ji,Wai, Sing Fai,Lee, Fuk Ki,Suen, Kar Man.

[11]Piglets with maternally derived antibodies from sows immunized with rAdV-SFV-E2 were completely protected against lethal CSFV challenge. Xia, Shui-Li,Du, Mingliang,Lei, Jian-Lin,Liu, Yan,Wang, Yimin,Ji, Shengwei,Xiang, Guang-Tao,Li, Lian-Feng,Cong, Xin,Luo, Yuzi,Shao, Lina,Qiu, Hua-Ji,Sun, Yuan.

[12]Efficacy of the marker vaccine rAdV-SFV-E2 against classical swine fever in the presence of maternally derived antibodies to rAdV-SFV-E2 or C-strain. Xia, Shui-Li,Xiang, Guang-Tao,Lei, Jian-Lin,Du, Mingliang,Wang, Yimin,Zhou, Mo,Liu, Yan,Ji, Shengwei,Wang, Ya-Lin,Luo, Yuzi,Sun, Yuan,Qiu, Hua-Ji.

[13]A novel alphavirus replicon-vectored vaccine delivered by adenovirus induces sterile immunity against classical swine fever. Sun, Yuan,Li, Hong-Yu,Tian, Da-Yong,Han, Qiu-Ying,Zhang, Xin,Li, Na,Qiu, Hua-Ji. 2011

[14]Study on the antioxidant ability and safety of products from transformation of flavones of buckwheat. Fang, Shan,Bian, Junsheng,Li, Hongmei,Li, Yunlong,Hu, Junjun. 2007

[15]Principles and Applications of Hyperspectral Imaging Technique in Quality and Safety Inspection of Fruits and Vegetables. Zhang Bao-hua,Zhang Bao-hua,Li Jiang-bo,Fan Shu-xiang,Huang Wen-qian,Zhang Chi,Wang Qing-yan,Xiao Guang-dong. 2014

[16]The study of 3-strand PP/PE composite monofilament Rope. Shi, Jian-gao,Yu, Wenwen,Liu, Yongli,Wang, Lei,Chen, Xiaoxue,Wang, Luming,Zhong, Wen-zhu. 2016

[17]Preparation of a standardized, efficacious agricultural H5N3 vaccine by reverse genetics. Liu, M,Wood, JM,Ellis, T,Krauss, S,Seiler, P,Johnson, C,Hoffmann, E,Humberd, J,Hulse, D,Zhang, Y,Webster, RG,Perez, DR.

[18]Long-term toxicity study on genetically modified corn with cry1Ac gene in a Wuzhishan miniature pig model. Zhang, Hongfu,Chen, Liang,Sun, Zhe,Zhong, Ruqing,Zhang, Hongfu,Chen, Liang,Liu, Quanwei,Tan, Shuyi,Yang, Xiaoguang.

[19]Research Process on Hyperspectral Imaging Detection Technology for the Quality and Safety of Grain and Oils. Yu Hong-wei,Wang Qiang,Liu Li,Shi Ai-min,Hu Hui,Liu Hong-zhi. 2016

[20]Attenuated live vaccine (Bartha-K16) caused pseudorabies (Aujeszky's disease) in sheep. Kong, Hanjin,Zhang, Keshan,Liu, Yongjie,Shang, Youjun,Liu, Xiangtao,Kong, Hanjin,Wu, Bin,Liu, Xiangtao.

作者其他论文 更多>>

微信小程序