Self-assembly of virus-like particles of rabbit hemorrhagic disease virus capsid protein expressed in Escherichia coli and their immunogenicity in rabbits

文献类型： 外文期刊

第一作者： Guo, Huimin

作者： Guo, Huimin;Zhu, Jie;Tan, Yonggui;Li, Chuanfeng;Chen, Zongyan;Liu, Guangqing;Sun, Shiqi

作者机构：

关键词： Rabbit hemorrhagic disease virus;VP60 gene;Virus-like particle;Vaccine

期刊名称：ANTIVIRAL RESEARCH （ 影响因子：5.97； 五年影响因子：5.801 ）

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收录情况： SCI

摘要： In this study, virus-like particles (VLPs) derived from rabbit hemorrhagic disease virus (RHDV) were evaluated for the development of a vaccine against RHDV infection. The VP60 gene was cloned and inserted into a pSMK expression vector containing a small ubiquitin-like modifier (SUMO) tag that can promote the soluble expression of heterologous proteins in Escherichia coli cells. After expression and purification of His-SUMO-VP60 and cleavage of the SUMO tag, we found that the RHDV VP60 protein had self-assembled into VLPs with a similar shape and smaller size compared with authentic RHDV capsid. Next, the antigenicity and immunogenicity of the VLPs were examined. The results showed that RHDV-specific responses were clearly induced in rabbits and that all rabbits in the VLP group survived while those in the negative control group died within 72 h post-infection. These results suggest that VLP-based RHDV could be a promising RHDV vaccine candidate. (C) 2016 Elsevier B.V. All rights reserved.

分类号： R37

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