Transcriptome analysis of root development in bottle gourd (Lagenaria siceraria)

文献类型： 外文期刊

第一作者： Yang, X. P.

作者： Yang, X. P.;Zhang, M.;Xu, J. H.;Liu, G.;Yao, X. F.;Li, P. F.;Zhu, L. L.

作者机构：

关键词： defense metabolites;Fusarium wilt;Illumina sequencing;Lagenaria siceraria Sen;transcriptome profile

期刊名称：XXIX INTERNATIONAL HORTICULTURAL CONGRESS ON HORTICULTURE: SUSTAINING LIVES, LIVELIHOODS AND LANDSCAPES (IHC2014): INTERNATIONAL SYMPOSIUM ON MOLECULAR BIOLOGY IN HORTICULTURE

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Bottle gourd is widely used as rootstock because of its highly resistant efficiency on Fusarium wilt. Previous work mainly focused on its resistance to Fusarium wilt and/or its effects on gene expression patterns in scions. However, little is known about its own metabolism, especially the early stage of the root development after Fusarium oxysporum inoculation. In this study, a cDNA library generated from root RNA sampled from bottle gourd seedling at 0 days after inoculation (DAI) and 10 DAI were sequenced using Illumina highseq 2000 platform. More than 41,831,589 high quality reads were generated and was assembled into 56,905 unigenes with an average sequence length of 735.11 base pairs. A total of 38,515 (67.7%) unigenes were annotated and of which 8,171 were assigned to specific metabolic pathways by the Kyoto Encyclopaedia of Genes and Genomes (KEGG). Of all unigenes, 2,469 genes were differentially expressed, including 1,294 up-regulated genes and 1,175 down-regulated genes. With Clusters ofOrthologous Groups of Proteins (COG) and Gene Ontology (GO) functional classifications, all differentially expressed-transcripts were grouped into 23 and 55 categories, respectively, which includes many functional categories, such as energy metabolism, signalling, transcription regulation and defence reactions. Genes involving the defence biosynthesis were analysed. 37 gene sequences encoding the defence metabolites were discovered in the transcriptome. Our study has provided a dataset that documents the dynamics of the root transcriptome at the early development stage. The transcriptome data also provides an important resource for understanding the formation of defence constituents in the root extract from bottle gourd.

分类号： S6

  • 相关文献

[1]Generation of transcriptome profiling and gene functional analysis in Gossypium hirsutum upon Verticillium dahliae infection. Zhang, Huachong,Qi, Fangjun,Jian, Guiliang.

[2]Genome-Wide Transcriptome Profiles of Rice Hybrids and Their Parents. Zhiguo, E.,Zhang, Yuping,Wang, Lei,Huang, Shanshan,Ge, Lei. 2014

[3]Identifying potential RNAi targets in grain aphid (Sitobion avenae F.) based on transcriptome profiling of its alimentary canal after feeding on wheat plants. Zhang, Min,Wang, Hui,Wang, Dahai,Ma, Youzhi,Xia, Lanqin,Zhou, Yuwen,Gao, Qiang,Jones, Huw Dylan. 2013

[4]Resistance to Fusarium oxysporum f. sp cubense tropical race 4 in African bananas. Molina, A. B.,Sinohin, V. O.,Fabregar, E. G.,Ramillete, E. B.,Yi, G.,Sheng, O.,Karamura, D.,Van den Bergh, I.,Viljoen, A.. 2016

[5]Carbon adaptation influence the antagonistic ability of Pseudomonas aeruginosa against Fusarium oxysporum f. sp melonis. Wang, Yanli,Sun, Guochang,Li, Bin,Tang, Qiaomei,Chen, Xiaoling,Xie, Guanlin,Li, Hongye,Yu, Rongrong,Wu, Zhiyi. 2011

[6]Soil Bacterial Community Was Changed after Brassicaceous Seed Meal Application for Suppression of Fusarium Wilt on Pepper. Ren, Gaidi,Ma, Yan,Guo, Dejie,Ren, Gaidi,Ma, Yan,Guo, Dejie,Ren, Gaidi,Ma, Yan,Guo, Dejie,Gentry, Terry J.,Hu, Ping,Pierson, Elizabeth A.,Gu, Mengmeng. 2018

[7]The Activities of Peroxidase and Polyphenoloxidase, and the Content of Soluble Protein in Fusaric Acid Resistant Chieh-qua during Infection with Fusarium oxysporum. Sun, Xiaowu,Wang, Yongfei,Ma, Sanmei,Wang, Lixian,Yu, Mengjie,Wang, Lixian,He, Xiaoming,Sun, Xiaowu. 2010

[8]Biofilm formation by Fusarium oxysporum f. sp cucumerinum and susceptibility to environmental stress. Li Peiqian,Pu Xiaoming,Huang Ning,Lin Birun,Pu Xiaoming,Shen Huifang,Zhang Jingxin,Huang Ning,Lin Birun. 2014

[9]Recent Occurrence of Fusarium oxysporum f. sp cubense Tropical Race 4 in Asia. Molina, A. B.,Sinohin, V. G.,Fabregar, E.,Yi, G.,Viljoen, A.. 2009

[10]Physiological Responses of Watermelon Grafted onto Bottle Gourd to Fusarium oxysporum f. sp niveum Infection. Zhang, M.,Yang, X. P.,Xu, J. H.,Liu, G.,Yao, X. F.,Li, P. F.. 2015

[11]An efficient protocol for the production of chit42 transgenic Furenzhi banana (Musa spp. AA group) resistant to Fusarium oxysporum. Hu, Chun-Hua,Wei, Yue-Rong,Huang, Yong-Hong,Yi, Gan-Jun,Hu, Chun-Hua,Wei, Yue-Rong,Huang, Yong-Hong,Yi, Gan-Jun. 2013

[12]Genetic analysis and chromosome mapping of resistance to Fusarium oxysporum f. sp niveum (FON) race 1 and race 2 in watermelon (Citrullus lanatus L.). Ren, Yi,Jiao, Di,Gong, Guoyi,Zhang, Haiying,Guo, Shaogui,Zhang, Jie,Xu, Yong,Ren, Yi,Jiao, Di,Gong, Guoyi,Zhang, Haiying,Guo, Shaogui,Zhang, Jie,Xu, Yong,Ren, Yi,Jiao, Di,Gong, Guoyi,Zhang, Haiying,Guo, Shaogui,Zhang, Jie,Xu, Yong,Ren, Yi,Jiao, Di.

[13]Development of InDel markers linked to Fusarium wilt resistance in cabbage. Lv, Hong-hao,Yang, Li-mei,Wang, Qing-biao,Wang, Xiao-wu,Fang, Zhi-yuan,Liu, Yu-mei,Zhuang, Mu,Zhang, Yang-yong,Lin, Yan,Yang, Yu-hong,Xie, Bing-yan,Liu, Bo,Liu, Ji-Sheng,Kang, Jun-gen.

[14]Impact of brassicaceous seed meals on the composition of the soil fungal community and the incidence of Fusarium wilt on chili pepper. Ma, Yan,Gentry, Terry,Hu, Ping,Pierson, Elizabeth,Gu, Mengmeng,Yin, Shixue.

[15]Bio-fertilizer application induces soil suppressiveness against Fusarium wilt disease by reshaping the soil microbiome. Xiong, Wu,Guo, Sai,Jousset, Alexandre,Li, Rong,Shen, Qirong,Xiong, Wu,Jousset, Alexandre,Kowalchuk, George A.,Xiong, Wu,Zhao, Qingyun,Wu, Huasong.

[16]Identification of defense-related genes in banana roots infected by Fusarium oxysporum f. sp cubense tropical race 4. Li, Weiming,Wang, Wei,Hu, Yulin,Mo, Yiwei,Sun, Dequan,Shi, Shengyou,Xie, Jianghui,Li, Weiming,Ge, Xuejun,Wu, Wei.

[17]Genetic analysis of Verticillium wilt resistance in a backcross inbred line population and a meta-analysis of quantitative trait loci for disease resistance in cotton. Jinfa Zhang,Jiwen Yu,Wenfeng Pei,Xingli Li,Joseph Said,Mingzhou Song,Soum Sanogo. 2015

[18]Mapping and analysis of a novel candidate Fusarium wilt resistance gene FOC1 in Brassica oleracea. Lv, Honghao,Fang, Zhiyuan,Yang, Limei,Zhang, Yangyong,Liu, Yumei,Zhuang, Mu,Yang, Yuhong,Xie, Bingyan,Liu, Bo,Liu, Jisheng,Wang, Xiaowu,Wang, Qingbiao,Kang, Jungen. 2014

[19]Pathogenic variation and molecular characterization of Fusarium species isolated from wilted sesame in China. Li Dong-hua,Wang Lin-hai,Zhang Yan-xin,Lv Hai-xia,Qi Xiao-qiong,Wei Wen-liang,Zhang Xiu-rong. 2012

[20]Rhizosphere soil properties and banana Fusarium wilt suppression influenced by combined chemical and organic fertilizations. Sun, Jianbo,Zou, Liangping,Li, Wenbin,Yang, Jinghao,Wang, Yuguang,Xia, Qiyu,Peng, Ming. 2018

作者其他论文 更多>>

微信小程序