Construction of a high-density SNP genetic map in fluecured tobacco based on SLAF-seq

文献类型： 外文期刊

第一作者： Gong, Daping

作者： Gong, Daping;Xu, Xiuhong;Wang, Chuanyi;Ren, Min;Wang, Chunkai;Chen, Mingli;Huang, Long;Xu, Xiuhong;Wang, Chunkai;Wang, Chunkai

作者机构：

关键词： Single nucleotide polymorphism;Genetic map;Quantitative trait locus;SLAF-seq;Tobacco

期刊名称：MOLECULAR BREEDING （ 影响因子：2.589； 五年影响因子：2.75 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Tobacco (Nicotiana tabacum L., 2n = 48) is an important agronomic crop and model plant. Fluecured tobacco is the most important type and accounts for approximately 80 % of tobacco production worldwide. The low genetic diversity of flue-cured tobacco impedes the construction of a high-density genetic linkage map using simple sequence repeat (SSR) markers and warrants the exploitation of single nucleotide polymorphic (SNP) markers from genomic regions. In this article, initially using specific locus-amplified fragment sequencing, we discovered 10,891 SNPs that were subsequently used as molecular markers for genetic map construction. Combined with SSR markers, a final high-density genetic map was generated containing 4215 SNPs and 194 SSRs distributed on 24 linkage groups (LGs). The genetic map was 2662.43 cM in length, with an average distance of 0.60 cM between adjacent markers. Furthermore, by mapping the SNP markers to the ancestral genomes of Nicotiana tomentosiformis and Nicotiana sylvestris, a large number of genome rearrangements were identified as occurring after the polyploidization event. Finally, using this novel integrated map and mapping population, two major quantitative trait loci (QTLs) were identified for flue-curing and mapped to the LG6 of tobacco. This is the first report of SNP markers and a SNP-based linkage map being developed in tobacco. The high-density genetic map and QTLs related to tobacco curing will support gene/QTL fine mapping, genome sequence assembly and molecular breeding in tobacco.

分类号： Q94

  • 相关文献

[1]High-density genetic map construction and gene mapping of pericarp color in wax gourd using specific-locus amplified fragment (SLAF) sequencing. Jiang, Biao,Liu, Wenrui,Xie, Dasen,Peng, Qingwu,He, Xiaoming,Lin, Yu'e,Liang, Zhaojun,Jiang, Biao,Liu, Wenrui,Xie, Dasen,He, Xiaoming. 2015

[2]High-Density Genetic Map Construction and Gene Mapping of Basal Branching Habit and Flowers per Leaf Axil in Sesame. Mei, Hongxian,Liu, Yanyang,Du, Zhenwei,Wu, Ke,Cui, Chengqi,Jiang, Xiaolin,Zhang, Haiyang,Zheng, Yongzhan. 2017

[3]Construction of a high-density genetic linkage map in pear (Pyrus communis x Pyrus pyrifolia nakai) using SSRs and SNPs developed by SLAF-seq. Wang, Long,Wu, Jun,Yin, Hao,Zhang, Shaoling,Wang, Long,Li, Xiugen,Wang, Lei,Xue, Huabai.

[4]Construction of a high-density genetic map for watermelon (Citrullus lanatus L.) based on large-scale SNP discovery by specific length amplified fragment sequencing (SLAF-seq). Shang, Jianli,Li, Na,Li, Nannan,Xu, Yongyang,Ma, Shuangwu,Wang, Jiming.

[5]Construction of a high-density genetic map and the X/Y sex-determining gene mapping in spinach based on large-scale markers developed by specific-locus amplified fragment sequencing (SLAF-seq). Qian, Wei,Fan, Guiyan,Liu, Dandan,Zhang, Helong,Wang, Xiaowu,Wu, Jian,Xu, Zhaosheng. 2017

[6]High-Density SNP Map Construction and QTL Identification for the Apetalous Character in Brassica napus L.. Wang, Xiaodong,Yu, Kunjiang,Li, Hongge,Peng, Qi,Chen, Feng,Zhang, Wei,Chen, Song,Hu, Maolong,Zhang, Jiefu,Wang, Xiaodong,Zhang, Jiefu,Chen, Feng. 2015

[7]Genome-wide association study for soybean cyst nematode resistance in Chinese elite soybean cultivars. Zhang, Jun,Li, Wei,Zhang, Yanwei,Zhang, Lifeng,Dai, Haiying,Xu, Ran,Wen, Zixiang,Wang, Dechun,Zhang, Jun.

[8]Identification of favorable SNP alleles and candidate genes for traits related to early maturity via GWAS in upland cotton. Junji Su,Chaoyou Pang,Hengling Wei,Libei Li,Bing Liang,Caixiang Wang,Meizhen Song,Hantao Wang,Shuqi Zhao,Xiaoyun Jia,Guangzhi Mao,Long Huang,Dandan Geng,Chengshe Wang,Shuli Fan. 2016

[9]High-Density Genetic Mapping Identifies New Major Loci for Tolerance to Low-Phosphorus Stress in Soybean. Zhang, Dan,Li, Hongyan,Chu, Shanshan,Lv, Haiyan,Wang, Jinshe,Zhang, Hengyou,Hu, Zhenbin,Yu, Deyue. 2016

[10]Rapid identification of rice blast resistance gene by specific length amplified fragment sequencing. Chen, Shen,Wang, Wen-juan,Su, Jing,Wang, Cong-ying,Feng, Ai-qing,Yang, Jian-yuan,Zeng, Lie-xian,Zhu, Xiao-yuan. 2016

[11]Cloning and Characterization of TaTGW-7A Gene Associated with Grain Weight in Wheat via SLAF-seq-BSA. Hu, Ming-Jian,Zhang, Hai-Ping,Liu, Kai,Cao, Jia-Jia,Wang, Sheng-Xing,Jiang, Hao,Wu, Zeng-Yun,Lu, Jie,Zhu, Xiao F.,Xia, Xian-Chun,Sun, Gen-Lou,Ma, Chuan-Xi,Chang, Cheng,Xia, Xian-Chun,Sun, Gen-Lou. 2016

[12]Construction of a High-Density Genetic Map Based on Large-Scale Marker Development in Mango Using Specific-Locus Amplified Fragment Sequencing (SLAF-seq). Luo, Chun,Shu, Bo,Yao, Quangsheng,Wu, Hongxia,Xu, Wentian,Wang, Songbiao. 2016

[13]Physical mapping and candidate gene prediction of fertility restorer gene of cytoplasmic male sterility in cotton. Zhao, Cunpeng,Zhao, Guiyuan,Geng, Zhao,Wang, Zhaoxiao,Wang, Kaihui,Liu, Suen,Zhang, Hanshuang,Guo, Baosheng,Geng, Junyi. 2018

[14]QTL Mapping by SLAF-seq and Expression Analysis of Candidate Genes for Aphid Resistance in Cucumber. Liang, Danna,Chen, Minyang,Qi, Xiaohua,Xu, Qiang,Zhou, Fucai,Chen, Xuehao,Liang, Danna. 2016

[15]Toward Identification of Black Lemma and Pericarp Gene Blp1 in Barley Combining Bulked Segregant Analysis and Specific-Locus Amplified Fragment Sequencing. Jia, Qiaojun,Liang, Zongsuo,Jia, Qiaojun,Liang, Zongsuo,Wang, Junmei,Zhu, Jinghuan,Hua, Wei,Shang, Yi,Yang, Jianming. 2017

[16]Construction of a high-density genetic map based on large-scale markers developed by specific length amplified fragment sequencing (SLAF-seq) and its application to QTL analysis for isoflavone content in Glycine max. Li, Bin,Tian, Ling,Zhang, Jingying,Han, Fenxia,Yan, Shurong,Wang, Lianzheng,Sun, Junming,Huang, Long,Zheng, Hongkun. 2014

[17]Genome-Wide Association Study Identifying Candidate Genes Influencing Important Agronomic Traits of Flax (Linum usitatissimum L.)Using SLAF-seq. Xie, Dongwei,Dai, Zhigang,Yang, Zemao,Tang, Qing,Su, Jianguang,Xie, Dongwei,Zhao, Debao,Yang, Xue,Zhang, Liguo,Sun, Jian. 2018

[18]Genome-Wide SNP Markers Based on SLAF-Seq Uncover Breeding Traces in Rapeseed (Brassica napus L.). Zhou, Qinghong,Zhou, Can,Fan, Shuying,Wu, Caijun,Fu, Donghui,Huang, Yingjin,Zheng, Wei,Mason, Annaliese S.. 2017

[19]Mining candidate genes associated with powdery mildew resistance in cucumber via super-BSA by specific length amplified fragment (SLAF) sequencing. Zhang, Peng,Zhu, Yuqiang,Wang, Lili,Chen, Liping,Zhou, Shengjun. 2015

[20]Analysis of a major rice blast resistance gene in the rice restorer line Hanghui 1179. Zhou, Jiyong,Xiao, Wuming,Chen, Zhiqiang,Wang, Wenjuan,Feng, Aiqing,Zhu, Xiaoyuan,Chen, Shen,Zhou, Jiyong.

作者其他论文 更多>>

微信小程序