文献类型： 外文期刊
第一作者： Zhou, Jun
作者： Zhou, Jun;Fan, Xudong;Dong, Yafeng;Zhang, Zun Ping;Ren, Fang;Hu, Guojun
作者机构：
期刊名称：ARCHIVES OF VIROLOGY （ 影响因子：2.574； 五年影响因子：2.466 ）
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年卷期：
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收录情况： SCI
摘要： To investigate the prevalence and genetic variation of grapevine fanleaf virus (GFLV) in China, 142 grapevine samples from 13 provinces and regions were tested using DAS-ELISA, RT-PCR, and nested RT-PCR. Of the samples, 38 % tested positive for GFLV by DAS-ELISA, and 26.8 % tested positive by RT-PCR and nested RT-PCR. Movement protein (MP) and coat protein (CP) gene PCR products were cloned and sequenced. The MP or CP nucleotide and protein sequences shared identities that ranged from 94.9 % to 100 %. Phylogenetic analysis revealed that Chinese GFLV isolates obtained in this study were distinct from the isolates reported in GenBank.
分类号： R37
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