The intergenic region of the maize defensin-like protein genes Def1 and Def2 functions as an embryo-specific asymmetric bidirectional promoter
文献类型: 外文期刊
第一作者: Liu, Xiaoqing
作者: Liu, Xiaoqing;Yang, Wenzhu;Li, Ye;Li, Suzhen;Zhou, Xiaojin;Zhao, Qianqian;Fan, Yunliu;Lin, Min;Chen, Rumei;Li, Suzhen
作者机构:
关键词: Bidirectional promoter;cis-elements;embryo specificity;immature embryo transient expression system;transgenic maize;truncation analysis
期刊名称:JOURNAL OF EXPERIMENTAL BOTANY ( 影响因子:6.992; 五年影响因子:7.86 )
ISSN:
年卷期:
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收录情况: SCI
摘要: Here we cloned and intensively characterized the first natural embryo-specific bidirectional promoter which could facilitate multi-gene expression in maize, and proposed a model of regulation of bidirectional transcription initiation.Bidirectional promoters are identified in diverse organisms with widely varied genome sizes, including bacteria, yeast, mammals, and plants. However, little research has been done on any individual endogenous bidirectional promoter from plants. Here, we describe a promoter positioned in the intergenic region of two defensin-like protein genes, Def1 and Def2 in maize (Zea mays). We examined the expression profiles of Def1 and Def2 in 14 maize tissues by qRT-PCR, and the results showed that this gene pair was expressed abundantly and specifically in seeds. When fused to either green fluorescent protein (GFP) or beta-glucuronidase (GUS) reporter genes, P (ZmBD1) , P (ZmDef1) , and P (ZmDef2) were active and reproduced the expression patterns of both Def1 and Def2 genes in transformed immature maize embryos, as well as in developing seeds of transgenic maize. Comparative analysis revealed that P-ZmBD1 shared most of the expression characteristics of the two polar promoters, but displayed more stringent embryo specificity, delayed expression initiation, and asymmetric promoter activity. Moreover, a truncated promoter study revealed that the core promoters only exhibit basic bidirectional activity, while interacting with necessary cis-elements, which leads to polarity and different strengths. The sophisticated interaction or counteraction between the core promoter and cis-elements may potentially regulate bidirectional promoters.
分类号: Q94
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