The intergenic region of the maize defensin-like protein genes Def1 and Def2 functions as an embryo-specific asymmetric bidirectional promoter

文献类型: 外文期刊

第一作者: Liu, Xiaoqing

作者: Liu, Xiaoqing;Yang, Wenzhu;Li, Ye;Li, Suzhen;Zhou, Xiaojin;Zhao, Qianqian;Fan, Yunliu;Lin, Min;Chen, Rumei;Li, Suzhen

作者机构:

关键词: Bidirectional promoter;cis-elements;embryo specificity;immature embryo transient expression system;transgenic maize;truncation analysis

期刊名称:JOURNAL OF EXPERIMENTAL BOTANY ( 影响因子:6.992; 五年影响因子:7.86 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: Here we cloned and intensively characterized the first natural embryo-specific bidirectional promoter which could facilitate multi-gene expression in maize, and proposed a model of regulation of bidirectional transcription initiation.Bidirectional promoters are identified in diverse organisms with widely varied genome sizes, including bacteria, yeast, mammals, and plants. However, little research has been done on any individual endogenous bidirectional promoter from plants. Here, we describe a promoter positioned in the intergenic region of two defensin-like protein genes, Def1 and Def2 in maize (Zea mays). We examined the expression profiles of Def1 and Def2 in 14 maize tissues by qRT-PCR, and the results showed that this gene pair was expressed abundantly and specifically in seeds. When fused to either green fluorescent protein (GFP) or beta-glucuronidase (GUS) reporter genes, P (ZmBD1) , P (ZmDef1) , and P (ZmDef2) were active and reproduced the expression patterns of both Def1 and Def2 genes in transformed immature maize embryos, as well as in developing seeds of transgenic maize. Comparative analysis revealed that P-ZmBD1 shared most of the expression characteristics of the two polar promoters, but displayed more stringent embryo specificity, delayed expression initiation, and asymmetric promoter activity. Moreover, a truncated promoter study revealed that the core promoters only exhibit basic bidirectional activity, while interacting with necessary cis-elements, which leads to polarity and different strengths. The sophisticated interaction or counteraction between the core promoter and cis-elements may potentially regulate bidirectional promoters.

分类号: Q94

  • 相关文献

[1]Identification and characterization of promoters specifically and strongly expressed in maize embryos. Liu, Xiaoqing,Tian, Jian,Zhou, Xiaojin,Chen, Rumei,Wang, Lei,Zhang, Chunyi,Zhao, Jun,Fan, Yunliu,Liu, Xiaoqing,Tian, Jian,Zhou, Xiaojin,Chen, Rumei,Wang, Lei,Zhang, Chunyi,Zhao, Jun,Fan, Yunliu.

[2]Chicken CCDC152 shares an NFYB-regulated bidirectional promoter with a growth hormone receptor antisense transcript and inhibits cells proliferation and migration. Lin, Shudai,Luo, Wei,Jiang, Mingya,Luo, Wen,Abdalla, Bahareldin Ali,Nie, Qinghua,Zhang, Xiquan,Lin, Shudai,Luo, Wei,Jiang, Mingya,Luo, Wen,Abdalla, Bahareldin Ali,Nie, Qinghua,Zhang, Xiquan,Zhang, Li. 2017

[3]Identification and functional characterization of bidirectional gene pairs and their intergenic regions in maize. Liu, Xiaoqing,Zhou, Xiaojin,Li, Ye,Tian, Jian,Zhang, Qiuxue,Li, Suzhen,Wang, Lei,Zhao, Jun,Chen, Rumei,Fan, Yunliu,Liu, Xiaoqing,Zhou, Xiaojin,Tian, Jian,Zhang, Qiuxue,Wang, Lei,Zhao, Jun,Chen, Rumei,Fan, Yunliu,Li, Ye,Li, Suzhen. 2014

[4]Isolation and Functional Characterization of Bidirectional Promoters in Rice. Wang, Rui,Zhu, Menglin,Yang, Mei,Zhou, Fei,Chen, Hao,Lin, Yongjun,Wang, Rui,Zhu, Menglin,Yang, Mei,Zhou, Fei,Chen, Hao,Lin, Yongjun,Yan, Yan. 2016

[5]Phylogeny and Expression Analyses Reveal Important Roles for Plant PKS III Family during the Conquest of Land by Plants and Angiosperm Diversification. Xie, Lulu,Zhang, Shifan,Zhang, Shujiang,Li, Fei,Zhang, Hui,Li, Guoliang,Wei, Yunxiao,Sun, Rifei,Liu, Pingli,Zhu, Zhixin. 2016

[6]Isolation, structural analysis, and expression characteristics of the maize (Zea mays L.) hexokinase gene family. Zhang, Zhongbao,Zhang, Jiewei,Chen, Yajuan,Li, Ruifen,Wang, Hongzhi,Ding, Liping,Wei, Jianhua,Zhang, Zhongbao,Zhang, Jiewei,Chen, Yajuan,Li, Ruifen,Wang, Hongzhi,Ding, Liping,Wei, Jianhua.

[7]Genome-wide analysis and identification of HAK potassium transporter gene family in maize (Zea mays L.). Zhang, Zhongbao,Zhang, Jiewei,Chen, Yajuan,Li, Ruifen,Wang, Hongzhi,Wei, Jianhua.

[8]Isolation, structural analysis, and expression characteristics of the maize TIFY gene family. Zhang, Zhongbao,Li, Xianglong,Han, Meng,Wu, Zhongyi,Zhang, Zhongbao,Li, Xianglong,Han, Meng,Wu, Zhongyi,Yu, Rong,Han, Meng,Wu, Zhongyi.

[9]Overexpression of a modified AM79 aroA gene in transgenic maize confers high tolerance to glyphosate. Ren Zhen-jing,Cao Gao-yi,Zhang Yu-wen,Liu Yan,Liu Yun-jun,Cao Gao-yi,Zhang Yu-wen,Liu Yan. 2015

[10]Assessment of transgene copy number and zygosity of transgenic maize overexpressing Cry1Ie gene with SYBRA (R) Green qRT-PCR. Zhang, Yuwen,Wang, Guoying,Liu, Yunjun,Zhang, Yuwen,Liu, Yan,Zhang, Jie,Wang, Jianhua.

[11]The Influence of Bt-Transgenic Maize Pollen on the Bacterial Diversity in the Midgut of Chinese Honeybees, Apis cerana cerana. Geng Li-li,Shu Chang-long,Song Fu-ping,Zhang Jie,Jiang Wei-yu,Lin Yi,Dai Ping-li,Zhou Ting,Lang Zhi-hong. 2013

[12]Developing transgenic maize (Zea mays L.) with insect resistance and glyphosate tolerance by fusion gene transformation. Lang Zhi-hong,Lu Wei,Zhu Li,Lin Min,Huang Da-fang,Zhang Jie,He Kang-lai. 2015

[13]The influence of Bt-transgenic maize pollen on the bacterial diversity in the midgut of Apis mellifera ligustica. Cui, Hong-Juan,Shu, Chang-Long,Song, Fu-Ping,Zhang, Jie,Dai, Ping-Li,Zhou, Ting,Lang, Zhi-Hong. 2013

[14]Efficacy Evaluation of Two Transgenic Maize Events Expressing Fused Proteins to Cry1Ab-Susceptible and -Resistant Ostrinia furnacalis (Lepidoptera: Crambidae). Liu, G. G.,Shen, Z. C.,Ye, G. Y.,He, K. L.,Peng, Y. F.. 2013

[15]Field observations of Ostrinia nubilalis eclosion and post-eclosion activity of females around their natal plants. Hu, Yang,Hu, Yang,Andow, David A.. 2011

[16]Overexpression of a novel Cry1Ie gene confers resistance to Cry1Ac-resistant cotton bollworm in transgenic lines of maize. Zhang, Yuwen,Ren, Yuan,Wang, Jianhua,Zhang, Yuwen,Liu, Yunjun,Liu, Yan,Wang, Guoying,Liang, Gemei,Song, Fuping,Bai, Shuxiong. 2013

[17]A 90-day toxicity study of GmTMT transgenic maize in Sprague-Dawley rats. Fang, Jin,Feng, Yongquan,Zhi, Yuan,Yu, Zhou,Jia, Xudong,Zhang, Lan.

[18]Complementary DNA (cDNA) cloning and functional verification of resistance to head smut disease (Sphacelotheca reiliana) of an NBS-LRR gene ZmNL in maize (Zea mays). Di, Hong,Deng, Yanxue,Dong, Xiaojie,Li, Rou,Zhou, Yu,Wang, Zhenhua,Yu, Tao.

作者其他论文 更多>>
  • Integrating processing factors and large-scale cabbage cultivation to understand the fate tendency and health risks of tolfenpyrad using deterministic and probabilistic models

    作者:Li, Tong;Li, Suzhen;Wu, Manni;Liu, Fengjiao;Chen, Zenglong;Li, Li;Li, Suzhen;Li, Tong;Cheng, Youpu;Ren, Xin

    关键词:Tolfenpyrad; Nationwide trials; Environmental fate; Processing; Health risk

  • Bioremediation of complex organic pollutants by engineered Vibrio natriegens

    作者:Su, Cong;Liu, Yong;Wang, Chen;Qu, Liwen;Dai, Junbiao;Cui, Haotian;Wang, Weiwei;Cheng, Zhenyu;Yang, Mengqiao;Li, Ye;He, Siyang;Xu, Ping;Tang, Hongzhi;Cui, Haotian;Wang, Weiwei;Cheng, Zhenyu;Yang, Mengqiao;Li, Ye;He, Siyang;Xu, Ping;Tang, Hongzhi;Cai, Yuejin;Zheng, Jiaxin;Zhao, Pingping;Dai, Junbiao

    关键词:

  • Genome-wide identification of SWEET family genes and functional analysis of NtSWEET12i under drought and saline-alkali stresses in tobacco

    作者:Song, Wenting;Xue, Luyao;Jin, Xiaoshan;Liu, Xiaoqing;Wu, Xinru;Cui, Mengmeng;Liu, Qianyu;Wang, Dawei;Song, Wenting;Xue, Luyao;Jin, Xiaoshan;Liu, Xiaoqing;Wu, Xinru;Cui, Mengmeng;Liu, Qianyu;Wang, Dawei;Song, Wenting;Chen, Xiaoxia

    关键词:Tobacco (Nicotiana tabacum L.); SWEET family genes; NtSWEET12i; Drought and saline-alkali stresses

  • EasyMetagenome: A user-friendly and flexible pipeline for shotgun metagenomic analysis in microbiome research

    作者:Bai, Defeng;Xun, Jiani;Ma, Chuang;Luo, Hao;Yang, Haifei;Hou, Huiyu;Lv, Hujie;Wan, Xiulin;Wang, Yao;Yousuf, Salsabeel;Zeng, Meiyin;Zhang, Tianyuan;Gao, Yunyun;Liu, Yong-Xin;Chen, Tong;Ma, Chuang;Yang, Haifei;Cao, Chen;Cao, Xiaofeng;Cui, Jianzhou;Deng, Yuan-Ping;Deng, Zhaochao;Yu, Hao;Zhang, Chunfang;Dong, Wenxin;Dong, Wenxue;Du, Juan;Fang, Qunkai;Fang, Wei;Fang, Yue;Luan, Yaning;Fu, Fangtian;Fu, Min;Fu, Yi-Tian;Gao, He;Ge, Jingping;Guo, Yuhao;Gong, Qinglong;Lou, Wenbo;Gu, Lunda;Yang, Li;Guo, Peng;Hai, Tang;Liu, Hao;He, Jieqiang;He, Zi-Yang;Huang, Can;Ji, Shuai;Jiang, ChangHai;Jiang, Gui-Lai;Jiang, Lingjuan;Jin, Ling N.;Li, Changchao;Kan, Yuhe;Kang, Da;Kou, Jin;Lam, Ka-Lung;Li, Chong;Li, Fuyi;Li, Liwei;Li, Miao;Li, Xin;Li, Ye;Li, Zheng-Tao;Zhu, Chengshuai;Liang, Jing;Mo, Jiayuan;Lin, Yongxin;Liu, Changzhen;Liu, Danni;Zhang, Jing;Chen, Shifu;Liu, Fengqin;Liu, Jia;Liu, Tianrui;Liu, Tingting;Wang, Xinlong;Liu, Xinyuan;Luo, Yuanyuan;Liu, Yaqun;Liu, Bangyan;Liu, Minghao;Lv, Hujie;Ma, Tengfei;Mai, Zongjiong;Niu, Dongze;Pan, Zhuo;Qi, Heyuan;Shi, Zhanyao;Song, Chunjiao;Sun, Fuxiang;Sun, Yan;Tian, Sihui;Wang, Guoliang;Wang, Hongyang;Wang, Hongyu;Wang, Huanhuan;Wang, Jing;Wang, Jun;Wang, Kang;Wang, Leli;Yao, Xiaofang;Wang, Shao-kun;Xiao, Zufei;Xing, Huichun;Xu, Yifan;Yang, Song;Yan, Shu-yan;Zhang, Yi-Bo;Yang, Yuanming;Lei, Yu;Yuan, Zhengrong;Zhang, Chunge;Zhang, Huimin;Zhang, Na;Zhang, Yupeng;Zhang, Zheng;Zhou, Mingda;Zhou, Yuanping;Zhu, Zhihao;Zhu, Lin;Zhu, Yue;Zou, Hongqin;Zuo, Anna;Dong, Wenxuan;Wen, Tao;Chen, Shifu;Chen, Shifu;Li, Guoliang

    关键词:metagenome; microbiome; microbiota; pipeline; visualization

  • Knocking out artificially selected gene GmAOC4H8 improves germination in soybean

    作者:Zhang, Wei;Li, Songsong;Xu, Wenjing;Wang, Qiong;Zhang, Hongmei;Liu, Xiaoqing;Chen, Xin;Chen, Huatao;Chen, Huatao;Xu, Donghe

    关键词:

  • Origin and stepwise evolution of vertebrate lungs

    作者:Li, Ye;Hu, Mingliang;Zheng, Jiangmin;Zhang, Fenghua;Hao, Jiaqi;Xue, Tingfeng;Zhu, Chenglong;Liu, Yuxuan;Zhao, Lei;Xu, Wenjie;Xin, Peidong;Feng, Chenguang;Wang, Wen;Qiu, Qiang;Wang, Kun;Zhang, Zhigang;Zhao, Yilin;Zhang, Zhigang;Zhao, Yilin;Wu, Baosheng;Li, Zhaohong;Wang, Wen;Wang, Kun

    关键词:

  • Efficient C25-Hydroxylation of Vitamin D3 Utilizing an Artificial Self-Sufficient Whole-Cell Cytochrome P450 Biocatalyst

    作者:Liang, Ziqi;Zhou, Qiao;Li, Yicheng;Yang, Xiaojun;Liang, Ziqi;Zhou, Qiao;Li, Yicheng;Liu, Xiaoqing;Shen, Yiwen;Tian, Jian;Wang, Xiaolu;Qin, Xing;Wang, Yuan;Luo, Huiying;Yao, Bin;Tu, Tao

    关键词:25-hydroxyvitamin D-3; P450s; self-sufficientP450s; local energetic frustration; linker engineering