Establishment and characterization of three embryonic cell lines of beet armyworm, Spodoptera exigua (Lepidoptera: Noctuidae)

文献类型： 外文期刊

第一作者： Su, Rui

作者： Su, Rui;Zheng, Gui-Ling;Wan, Fang-Hao;Li, Chang-You;Wan, Fang-Hao

作者机构：

关键词： Spodoptera exigua;Embryonic cell line;Mitochondrial CO I gene;Nucleopolyhedrovirus;Apoptosis

期刊名称：CYTOTECHNOLOGY （ 影响因子：2.058； 五年影响因子：2.362 ）

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收录情况： SCI

摘要： Three cell lines (QAU-Se-E-1, -2 and -3, or Se-1, -2 and -3 for short) were established from eggs of beet armyworm (Spodoptera exigua) that have been passaged stably for more than 60 times in TNM-FH medium supplemented with 10 % fetal bovine serum. The cell lines consisted of round and spindle-shaped cells. The round cells accounted for 96.82, 84.34 and 83.16 % of the cells in the three cell lines, respectively, with cell diameters of 16.21 +/- 0.72, 15.63 +/- 0.58 and 13.06 +/- 0.44 mu m. Random amplified polymorphic DNA and analysis of the CO I gene showed that the three cell lines were all derived from S. exigua. Growth curves at passage 30 were determined and the results showed that the cell population doubling times were 59.03, 49.08 and 49.91 h, respectively. The three cell lines can be infected by S. exigua multiple nucleopolyhedrovirus (SeMNPV). Se-3 was extremely susceptible to the virus with an infection rate of 97.52 % 4 days after the inoculation and produced 2.02 x 10(6) OBs per mL of culture. Flow cytometry analysis showed that some of Se-1 and Se-2 cells had apoptosis after infection, whereas Se-3 cells did not. Bioassays showed that the virulence of the SeMNPV proliferated from Se-3 was similar to that from the insect with LC50 of 5.55 x 10(5) and 2.64 x 10(5) OBs/mL. Therefore, the cell lines can be used to study the SeMNPV-host interactions and mechanisms underlying the interactions.

分类号： Q2`Q1

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