The role of Ca2+ mediated signaling pathways on the effect of taurine against Streptococcus uberis infection

文献类型： 外文期刊

第一作者： Dai, Bin

作者： Dai, Bin;Liu, Ming;Zhang, Yuanshu;Xu, Yuanyuan;Miao, Jinfeng;Zhang, Jinqiu;Lu, Jinye;Yin, Yulong

作者机构：

关键词： Streptococcus uberis;Taurine;Ca2+;Mouse mammary epithelial cell

期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：3.293； 五年影响因子：3.599 ）

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收录情况： SCI

摘要： To provide insight into the mechanisms of taurine attenuation of pro-inflammatory response in mouse mammary epithelial cell line (EpH(4)-Ev, purchased by ATCC, USA) after Streptococcus uberis (S. uberis, 0140J) challenge, we infected MECs with S. uberis (2.5 x 10(7) cfu mL(-1), MOI = 10) for 3 h and quantified changes in TLR-2 and calcium (Ca2+) mediated signaling pathways. The results indicate that S. uberis infection significantly increases the expression of TLR-2, intracellular Ca2+ levels, PLC-gamma 1 and PKC-alpha, the activities of transcription factors NF-kappa B and NFAT, and related cytokines (TNF-alpha, IL-beta, IL-6, G-CSF, IL-2, KC, IL-15, FasL, MCP-1, and LIX) in culture supernatants. Taurine administration downregulated all these indices, the activities of NF-kappa B and NFAT. Cytokine secretions were similar using special PKC inhibitor Go 6983 and NFAT inhibitor VIVIT. Our data indicate that S. uberis infection induces pro-inflammatory response of MECs through a TLR-2 mediated signaling pathway. In addition, taurine can prevent MEC damage by affecting both PLC-gamma 1-Ca2+-PKC-alpha-NF-kappa B and PLC-gamma 1-Ca2+-NFATs signaling pathways. This is the first report to demonstrate the mechanisms of taurine attenuated pro-inflammatory response in MECs after S. uberis challenge. (C) 2016 Elsevier B.V. All rights reserved.

分类号： S8

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