THE METALLOTHIONEIN GENE FROM THE ORIENTAL RIVER PRAWN MACROBRACHIUM NIPPONENSE (DE HAAN, 1849): CHARACTERIZATION AND EXPRESSION IN RESPONSE TO HYPDXIA AND REOXYGENATION

文献类型： 外文期刊

第一作者： Sun, Shengming

作者： Sun, Shengming;Gu, Zhimin;Fu, Hongtuo;Zhu, Jian

作者机构：

关键词： Metallothionein;Macrobrachium nipponense;hypoxia;reoxygenation

期刊名称：CRUSTACEANA （ 影响因子：0.529； 五年影响因子：0.65 ）

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收录情况： SCI

摘要： Since mammals make use of metallothioneins (MTs) to protect against hypoxia-generated reactive oxygen species (ROS), here we investigate whether MTs have the same effect in the Oriental river prawn Macrobrachium nipponense (De Haan, 1849). Levels of M. nipponense MT (MnMT) mRNA in different tissues were analysed using a real-time quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR). We found that MnMT is differentially expressed in the haemocytes, intestine, gills, heart, hepatopancreas and muscle. The highest levels of expression occurred in the hepatopancreas and heart. The results of the qRT-PCR and Western blot indicated that MnMT mRNA and protein expression in the hepatopancreas increased significantly in response to hypoxia 24 h and followed by 1 h reoxygenation compared to normoxia. Similarly, there were also significant increases in respiratory bursts and ROS production in the hepatopancreas. The MT protein possibly has a protective effect against ROS generated during hypoxia and reoxygenation stimuli.

分类号： Q96

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