Rapid Isolation and Determination of Flavones in Biological Samples Using Zinc Complexation Coupled with High-Performance Liquid Chromatography
文献类型: 外文期刊
第一作者: Sun, Chenghe
作者: Sun, Chenghe;Wang, Hecheng;Wang, Yingping;Xiao, Shengyuan;Sun, Chenghe;Wang, Hecheng;Xiao, Shengyuan
作者机构:
关键词: Scutellaria baicalensis;dried blood spot;metal complexation;high-performance liquid chromatography;chlorophyll;heme
期刊名称:MOLECULES ( 影响因子:4.411; 五年影响因子:4.587 )
ISSN:
年卷期:
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收录情况: SCI
摘要: Chlorophyll-type contaminants are commonly encountered in the isolation and determination of flavones of plant aerial plant parts. Heme is also a difficult background substance in whole blood analysis. Both chlorophyll and heme are porphyrin type compounds. In this study, a rapid method for isolating flavones with 5-hydroxyl or ortho-hydroxyl groups from biological samples was developed based on the different solubilities of porphyrin-metal and flavone-metal complexes. It is important that other background substances, e.g., proteins and lipids, are also removed from flavones without an additional processing. The recoveries of scutellarin, baicalin, baicalein, wogonoside and wogonin, which are the primary constituents of Scutellaria baicalensis (skullcaps) were 99.65% +/- 1.02%, 98.98% +/- 0.73%, 99.65% +/- 0.03%, 97.59% +/- 0.09% and 95.19% +/- 0.47%, respectively. As a sample pretreatment procedure, this method was coupled to high-performance liquid chromatography (HPLC) with good separation, sensitivity and linearity and was applied to determine the flavone content in different aerial parts of S. baicalensis and in dried blood spot samples.
分类号: O62
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