Capsid proteins from PCV2a genotype confer greater protection against a PCV2b strain than those from PCV2b genotype in pigs: evidence for PCV2b strains becoming more predominant than PCV2a strains from 2000 to 2010s

文献类型： 外文期刊

第一作者： Huang, Liping

作者： Huang, Liping;Wang, Yiping;Wei, Yanwu;Chen, Dongjie;Liu, Dan;Du, Wenjuan;Xia, Deli;Wu, Hongli;Feng, Li;Liu, Changming

作者机构：

关键词： Porcine circovirus type 2;PCV2a;PCV2b;Capsid protein;Immunogenicity

期刊名称：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：4.813； 五年影响因子：4.697 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Two major porcine circovirus type 2 (PCV2) genotypes, PCV2a and PCV2b, are recognized. PCV2a was predominant in the global pig population until 2000 while PCV2b became predominant from 2003 onward. The aim of this study was to analyze the immune protection conferred by two PCV2a and two PCV2b capsid proteins (Caps) in pigs challenged with a mutant PCV2b/YJ (mPCV2b/YJ) strain. Pigs vaccinated with PCV2a/LG-Cap and PCV2a/CL-Cap elicited significantly higher levels of PCV2-specific antibodies and neutralizing antibodies compared with PCV2b/JF-Cap and mPCV2b/YJ-Cap. Following a mPCV2b/YJ challenge, no viremia was detected in the PCV2a/LG-Cap and PCV2a/CL-Cap groups, while viremias were found in 20 and 40 % of the pigs in the PCV2b/JF-Cap and mPCV2b/YJ-Cap groups, respectively. Viral loads in the inguinal lymph nodes of pigs from the PCV2b/JF-Cap and mPCV2b/YJ-Cap groups were significantly higher than those in the PCV2a/LG-Cap and PCV2a/CL-Cap groups, but significantly lower than those of the challenge control group. Furthermore, PCV2 antigens were not detected in the inguinal lymph nodes of pigs from commercial vaccine groups, as well as the PCV2a/LG-Cap and PCV2a/CL-Cap groups, but were found in the challenge control (100 %, 5/5), PCV2b/JF-Cap (20 %, 1/5), and mPCV2b/YJ-Cap (20 %, 1/5) groups. These findings suggest that mPCV2b/YJ-Cap and PCV2b/JF-Cap were less immunogenic than PCV2a/LG-Cap and PCV2a/CL-Cap. We speculate that a genotypic shift from PCV2a to PCV2b might be the result of the majority of PCV2a strains being more immunogenic than the majority of PCV2b strains. These results provide a possible explanation for why PCV2b strains are more likely to cause epidemics than PCV2a strains. It tells us that PCV2 pathogenesis may be associated with its immunogenicity to some extent.

分类号： Q939.9`Q81

  • 相关文献

[1]High-level expression and immunogenicity of a porcine circovirus type 2 capsid protein through codon optimization in Pichia pastoris. Tu, Yabin,Wang, Yanqun,Wang, Gang,Wu, Jianan,Liu, Yonggang,Wang, Shujie,Jiang, Chenggang,Cai, Xuehui.

[2]Antigenic subtyping and epitopes' competition analysis of porcine circovirus type 2 using monoclonal antibodies. Saha, Dipongkor,Huang, Liping,Bussalleu, Eva,Lefebvre, David J.,Fort, Maria,Nauwynck, Hans J.,Huang, Liping,Van Doorsselaere, Jan.

[3]A commercial PCV2a-based vaccine is effective in protection from experimental challenge of PCV2 mutant with two amino acids elongation in capsid protein. Guo, Long-Jun,Fu, Yu-Jie,Huang, Li-Ping,Wang, Yi-Ping,Wei, Yan-Wu,Wu, Hong-Li,Liu, Chang-Ming,Fu, Yu-Jie.

[4]Synthesis of empty capsid-like particles of Asia I foot-and-mouth disease virus in insect cells and their immunogenicity in guinea pigs. Cao, Yimei,Lu, Zengjun,Sun, Jiachuan,Bai, Xingwen,Sun, Pu,Bao, Huifang,Chen, Yingli,Guo, Jianhong,Li, Dong,Liu, Xiangtao,Liu, Zaixin.

[5]Identification of a New Antigen Epitope in the Nuclear Localization Signal Region of Porcine Circovirus Type 2 Capsid Protein. Guo, Longjun,Lu, Yuehua,Huang, Liping,Wei, Yanwu,Liu, Changming. 2011

[6]Self-assembly of virus-like particles of canine parvovirus capsid protein expressed from Escherichia coli and application as virus-like particle vaccine. Xu, Jin,Guo, Hui-Chen,Wei, Yan-Quan,Dong, Hu,Han, Shi-Chong,Ao, Da,Sun, De-Hui,Wang, Hai-Ming,Sun, Shi-Qi,Xu, Jin,Guo, Hui-Chen,Wei, Yan-Quan,Dong, Hu,Han, Shi-Chong,Ao, Da,Sun, De-Hui,Wang, Hai-Ming,Sun, Shi-Qi,Xu, Jin,Ao, Da,Cao, Sui-Zhong.

[7]Immunogenicity of porcine circovirus type 2 nucleic acid vaccine containing CpG motif for mice. Li, Jun,Yu, Jiang,Xu, Shaojian,Shi, Jianli,Xu, Shengnan,Wu, Xiaoyan,Peng, Zhe,Zhang, Lingling,Zheng, Shuxuan,Yuan, Xiaoyuan,Cong, Xiaoyan,Sun, Wenbo,Cheng, Kaihui,Du, Yijun,Wu, Jiaqiang,Wang, Jinbao,Fu, Fang. 2016

[8]High expression of capsid protein of red-spotted grouper nervous necrosis virus in an avian cell line requires viral RNA2 non-coding regions. Huang, J. N.,Lin, L.,Weng, S. P.,He, J. G.. 2007

[9]Development and application of an indirect enzyme-linked immunosorbent assay based on recombinant capsid protein for the detection of mink circovirus infection. Ge, Junwei,Cui, Xingyang,Shi, Yunjia,Wei, Chengwei,Wen, Shanshan,Xia, Shuang,Zhao, Lili,Chen, Hongyan,Ge, Junwei. 2018

[10]Codon optimization of the rabbit hemorrhagic disease virus (RHDV) capsid gene leads to increased gene expression in Spodoptera frugiperda 9 (Sf9) cells. Gao, Jingpeng,Meng, Chunchun,Chen, Zongyan,Li, Chuanfeng,Liu, Guangqing.

[11]Identification of a linear epitope on the capsid protein of classical swine fever virus. Zhang, Xin,Sun, Yuan,Li, Su,Li, Na,Yang, Shuo,He, Fan,Huang, Jun-Hua,Ling, Li-Jun,Qiu, Hua-Ji,Xu, Jia. 2011

[12]Preparation and Immunological Identification of Monoclonal Antibody Against JSRV-CA. Luo Jun-Rong,Siriguleng,Liu Xiao-Hui,Ma Xue-En,Luo Jun-Rong,Zhou Jian-Hua. 2009

[13]A DNA-launched reverse genetics system for rabbit hemorrhagic disease virus reveals that the VP2 protein is not essential for virus infectivity. Liu, Guangqing,Ni, Zheng,Yun, Tao,Yu, Bin,Chen, Liu,Zhao, Wei,Hua, Jionggang,Chen, Jianping.

[14]High level soluble expression and one-step purification of IBDV VP2 protein in Escherichia coli. Jiang, Dawei,Zhang, Gaiping,Yang, Guoyu,Song, Yapeng,Wang, Guoqiang,Wang, Jucai,Zhao, Baolei,Jiang, Dawei,Liu, Yunchao,Zhang, Gaiping,Ji, Pengchao,Liu, Chang,Song, Yapeng,Su, Yunfang,Wang, Guoqiang,Wang, Jucai,Zhao, Baolei,Deng, Ruiguang,Wang, Aiping,Chen, Yumei.

[15]Identification and characterization of a common B-cell epitope on EIAV capsid proteins. Hu, Zhe,Chang, Hao,Chu, Xiaoyu,Li, Shuang,Wang, Meiyue,Wang, Xiaojun.

[16]Development of antigen capture ELISA for the quantification of EIAV p26 protein. Hu, Zhe,Chang, Hao,Ge, Man,Lin, Yuezhi,Wang, Xuefeng,Guo, Wei,Wang, Xiaojun. 2014

[17]Nuclear localization signal regulates porcine circovirus type 2 capsid protein nuclear export through phosphorylation. Hou, Qiang,Hou, Shaohua,Jia, Hong,Xin, Ting,Jiang, Yitong,Guo, Xiaoyu,Zhu, Hongfei,Chen, Qing. 2018

[18]Co-existence of multiple strains of porcine circovirus type 2 in the same pig from China. Zhai, Shao-Lun,Chen, Sheng-Nan,Wei, Wen-Kang,Zhai, Shao-Lun,Wei, Zu-Zhang,Zhang, Jian-Wu,Huang, Lv,Lin, Tao,Yuan, Shi-Shan,Long, Jin-Xue,Zhai, Shao-Lun,Chen, Sheng-Nan,Yue, Cheng,Ran, Duo-Liang. 2011

[19]Porcine circovirus type 2 in China: an update on and insights to its prevalence and control. Zhai, Shao-Lun,Sun, Bei-Bei,Deng, Su-Fang,Hu, Jun,Luo, Man-Lin,Zhai, Shao-Lun,Xu, Zhi-Hong,Lv, Dian-Hong,Wen, Xiao-Hui,Yuan, Jie,Wei, Wen-Kang,Chen, Sheng-Nan,Tang, Man-Hua,Wang, Feng-Guo,Li, Xiao-Jing. 2014

[20]Construction and characterization of porcine circovirus type 2 carrying a genetic marker strain. Liu, Changming,Wei, Yanwu,Zhang, Caofan,Lu, Yueha,Kong, Xiangang. 2007

作者其他论文 更多>>

微信小程序