Differential analysis of the cytochrome p450 acaricide-resistance genes in Panonychus citri (Trombidiformes: Tetranychidae) strains
文献类型: 外文期刊
第一作者: Jiang, Gaofei
作者: Jiang, Gaofei;Chen, Fei;Ran, Chun;Zhang, Yunfei;Li, Junli;Li, Xiaojiao;Yue, Jiansu;Ran, Chun;Jiang, Gaofei;Zhang, Yunfei;Chen, Fei;Li, Junli;Li, Xiaojiao;Yue, Jiansu;Liu, Haoqiang;Li, Hongjun;Ran, Chun
作者机构:
关键词: citrus red mite;differential analysis;cytochrome P450;hexythiazox resistance;miticide resistance;RT-PCR analysis
期刊名称:FLORIDA ENTOMOLOGIST ( 影响因子:1.425; 五年影响因子:1.595 )
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收录情况: SCI
摘要: The citrus red mite, Panonychus citri (McGregor) (Trombidiformes: Tetranychidae), a highly destructive pest in citrus groves around the world, has developed resistance to many registered acaricides. Hexythiazox is a selective miticide that has been widely used to control citrus mites in a variety of crops. Forty-six cytochrome P450 mono-oxygenase genes related to general pesticide resistance in other insect species were obtained from the transcriptomes of the hexythiazox-resistant (RR) and hexythiazox-susceptible (SS) strains of P. citri and divided into 4 clans, 15 families and 24 subfamilies. Sequence analyses of each CYP resulted in detection of 3 mutationsin the CYP307A1 gene (841-A to C, 1395-T to C, 1491-T to C) that differed between the 2 strains. Only the change at an amino acid position (278-lysine to glutamine) resulted in a sense mutation. One SNP site was also detected in CYP381A2 (40-A to T) causing a sense amino acid mutation (14-threonine to serine). Seven of these P450s belonged to the CYP2 clan, CYP3 clan and CYP4 clan based on digital gene expression (DGE) library sequencing with a vertical bar log(2) ratio vertical bar value greater than 2, but there were no significant differences revealed by qRT-PCR analysis. This study provides essential information for future research on the hexythiazox-resistance mechanism of P. citri. More methods are needed to further elucidate the molecular mechanisms of resistance to hexythiazox in P. citri.
分类号: Q96
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