Role of autophagy in cellular response to infection with Orf virus Jilin isolate

文献类型: 外文期刊

第一作者: Lan, Yungang

作者: Lan, Yungang;Song, Deguang;He, Wenqi;Zhang, Di;Huang, Houshuang;Bi, Jingying;Gao, Feng;Zhao, Kui;Wang, Gaili;Gao, Feng

作者机构:

关键词: ORFV;OFTu cells;Autophagy;Autophagic flux

期刊名称:VETERINARY MICROBIOLOGY ( 影响因子:3.293; 五年影响因子:3.599 )

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收录情况: SCI

摘要: Autophagy is a conserved catabolic process of the cell, which has been described to be involved in the development of various viral diseases. However, the role of autophagy in Orf virus (ORFV) replication remains unknown. In this study, we provide the first evidence that ORFV infection triggered autophagy in primary ovine fetal turbinate cells (OFTu) based on the appearance of abundant double- and single-membrane vesicles, the accumulation of LC3 fluorescent puncta, the enhancement of LC3-I/-II conversion, and autophagic flux. Moreover, modulation of ORFV-induced autophagy by rapamycin (RAPA), Earle's balanced salts solution (EBSS), chloroquine (CQ) or 3-methyladenime (3-MA) does not affect virus production. In conclusion, these results suggest that autophagy can be induced in host cells by ORFV infection, but which maybe not essential for ORFV replication. (C) 2016 Elsevier B.V. All rights reserved.

分类号: S8

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