A new cell line derived from embryonic tissues of Holotrichia parallela (Coleoptera:Scarabaeidae)

文献类型： 外文期刊

第一作者： Li, Miao-Miao

作者： Li, Miao-Miao;Zheng, Gui-Ling;Su, Rui;Wan, Fang-Hao;Li, Chang-You;Li, Miao-Miao;Wan, Fang-Hao

作者机构：

关键词： Holotrichia parallela;Embryonic cell line;Biological characteristics

期刊名称：IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL （ 影响因子：2.416； 五年影响因子：2.117 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Holotrichia parallela is an important agricultural underground insect pest and also an edible and medicinal insect. Establishing a new cell line of H. parallela will provide a rapid and convenient tool for the studies on its physiology, pathology, and gene functions. In this study, by using the embryonic tissue of H. parallela as the material, we established a new cell line named Hp-E-1. The microscopic observation of its morphological characteristics revealed that its cellular morphology was mainly in the spherical morphology with a mean cellular diameter of 17.71 +/- 2.34 mu m, accounting for 67% of the total cells. The spindle-shaped cells accounted for 33% of the total cells with a mean size of 23.51 +/- 4.37 x 13.98 +/- 2.05 mu m. The chromosomal number varied from 7 to 40, with about 50% of the cells having a diploid chromosome number of 2n = 20. Random amplified polymorphic DNA (RAPD) analysis indicated that the profiles of PCR-amplified fragments of this cell line were basically similar to those of the embryonic tissues of H. parallela but were obviously different from those of cell line BTI-Tn5B1-4 of Trichoplusia ni and cell line Sf-9 of Spodoptera frugiperda. The DNA fragment encoding mitochondrial cytochrome C oxidase subunit I (COI) gene of this cell line shared 99.7% homology with that of the embryonic tissue of H. parallela, confirming that this cell line is indeed derived from H. parallela. The results of growth curve measurement indicated that the population doubling time of this cell line was 136.7 h. Cell line Hp-E-1 could not be infected by three viruses Autographa californica multiple nucleopolyhedrovirus (AcMNPV), Bombyx mori nucleopolyhedrovirus (BmNPV), and Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV).

分类号： Q95`Q2

  • 相关文献

[1]Development and characterization of a continuous embryonic cell line from turbot (Scophthalmus maximus). Chen, SL,Ren, GC,Sha, ZX,Hong, YH. 2005

[2]Development and characterization of a cell line from the embryos of half smooth tongue sole (Cynoglossus semilaevis). Sha Zhenxia,Ren Guocheng,Wang Xianli,Wang Na,Chen Songlin,Ren Guocheng,Wang Xianli. 2010

[3]Establishment and characterization of three embryonic cell lines of beet armyworm, Spodoptera exigua (Lepidoptera: Noctuidae). Su, Rui,Zheng, Gui-Ling,Wan, Fang-Hao,Li, Chang-You,Wan, Fang-Hao.

[4]Assembling of Holotrichia parallela (dark black chafer) midgut tissue transcriptome and identification of midgut proteins that bind to Cry8Ea toxin from Bacillus thuringiensis. Shu, Changlong,Tan, Shuqian,Yin, Jiao,Geng, Lili,Song, Fuping,Li, Kebin,Zhang, Jie,Soberon, Mario,Bravo, Alejandra,Liu, Chunqing.

[5]Construction of a Bacillus thuringiensis engineered strain with high toxicity and broad pesticidal spectrum against coleopteran insects. Liu, Jingjing,Yan, Guixin,Shu, Changlong,Zhao, Can,Song, Fuping,Zhou, Lin,Ma, Junlan,Zhang, Jie,Liu, Chunqin,Huang, Dafang.

[6]Characterization of a novel cry8 gene specific to Melolonthidae pests: Holotrichia oblita and Holotrichia parallela. Yan, Guixin,Zhang, Jie,Song, Fuping,Wang, Rongyan,Feng, Shuliang,Huang, Dafang.

[7]Characterization of one novel cry8 gene from Bacillus thuringiensis strain Q52-7. Liu, Rongmei,Zhang, Qingli,Zhao, Shiyuan,Shao, Gaoxiang,Zhang, Xiaofeng,Gao, Jiguo,Li, Haitao,Shu, Changlong.

[8]Genomic sequencing identifies novel Bacillus thuringiensis Vip1/Vip2 binary and Cry8 toxins that have high toxicity to Scarabaeoidea larvae. Zhang, Yanrui,Shu, Changlong,Song, Fuping,Zhang, Jie,Crickmore, Neil,Wang, Qinglei,Du, Lixin.

[9]Controlled Release Study on Microencapsulated Mixture of Fipronil and Chlorpyrifos for the Management of White Grubs (Holotrichia parallela) in Peanuts (Arachis hypogaea L.). Yang, Daibin,Yan, Xiaojing,Yuan, Huizhu,Li, Guangxing. 2014

[10]A chimeric cry8Ea1 gene flanked by MARs efficiently controls Holotrichia parallela. Geng, Lili,Chi, Jing,Shu, Changlong,Song, Fuping,Zhang, Jie,Gresshoff, Peter M.,Huang, Dafang. 2013

[11]Discovery and characterization of endo-xylanase and beta-xylosidase from a highly xylanolytic bacterium in the hindgut of Holotrichia parallela larvae. Xu, Jing,Zhang, Hongyu,Sheng, Ping,Xu, Jing,Zhang, Hongyu,Saccone, Giuseppe,Li, Kebin. 2014

[12]Isolation and functional analysis of the pathogenicity-related gene VdPR3 from Verticillium dahliae on cotton. Zhang, Ya-Lin,Li, Zhi-Fang,Feng, Zi-Li,Feng, Hong-Jie,Zhao, Li-Hong,Shi, Yong-Qiang,Hu, Xiao-Ping,Zhu, He-Qin.

[13]Establishment of Big Bone chicken fibroblast cell bank and study of its biological characteristics. Su, Xiaohua,Jin, Dapeng,Yue, Hua,Pu, Yabin,Ma, Yuehui,Guan, Weijun,Su, Xiaohua,Li, Chunying,Yue, Hua,Jin, Dapeng. 2011

[14]Biological characterization of sheep kidney-derived mesenchymal stem cells. Ji, Meng,Bai, Chunyu,Fan, Ya'nan,Ma, Caiyun,Li, Xiangchen,Guan, Weijun,Li, Lu. 2016

[15]Sensitivity to fluopicolide of wild type isolates and biological characteristics of fluopicolide-resistant mutants in Pseudoperonospora cubensis. Wang, Wenqiao,Yan, Lei,Meng, Runjie,Zhao, Jianjiang,Zhang, Xiaofeng,Han, Xiuying,Ma, Zhiqiang. 2014

[16]Establishment and Biological Characteristic Research of Yuxizhiwei sheep Fibroblast Cell Bank. Wang, Hui,Li, Xiangchen,Li, Changli,Zhang, Wenxiu,Guan, Weijun,Ma, Yuehui,Wang, Hui. 2012

[17]Establishment and characterization of a Chinese Game chicken embryo fibroblast bank. Li, Xiangchen,Bai, Chunyu,Su, Xiaohua,Jin, Dapeng,Zhao, Qianjun,He, Xiaohong,Guan, Weijun,Ma, Yuehui,Bai, Chunyu,Jin, Dapeng,Su, Xiaohua.

[18]Establishment and biological characteristics of Ujumqin sheep fibroblast line. Na, Ri Su,Zhao, Qian Jun,Jin, Da Peng,Su, Xiao Hua,Chen, Xian Wei,Guan, Wei Jun,Ma, Yue Hui,Jin, Da Peng,Su, Xiao Hua,Chen, Xian Wei.

[19]Establishment and biological characteristics of Qingyuan partridge chicken fibroblast line. Na, R. S.,Bai, C. Y.,Jin, D. P.,Su, X. H.,Feng, B. G.,Guan, W. J.,Ma, Y. H.,Jin, D. P.,Su, X. H.,Feng, B. G..

[20]Isolation and biological characterization of a novel type of pulmonary mesenchymal stem cells derived from Wuzhishan miniature pig embryo. Ma, Caiyun,Liu, Hao,Yang, Jinjuan,Li, Xiangchen,Liu, Changqing,Guan, Weijun,Guo, Yu,Liu, Changqing,Guo, Yu,Wang, Kunfu.

作者其他论文 更多>>

微信小程序